FastQCFastQC Report
Mon 15 Aug 2016
SRR2921341_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921341_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1372567
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31590.23015269928535362No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24580.17908051118816057No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22380.16305214973112422No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25200.062.372711
GTATCAA42550.048.878641
GTACATG78500.039.208211
TACATGG82300.037.434872
CATGGGG68650.036.284524
ACATGGG84650.036.1844373
ATGGGGG46050.035.327975
TCAACGC57850.034.817614
TATCAAC60050.034.2366872
ATCAACG58900.033.994583
CAACGCA59700.033.539045
AACGCAG62150.032.598066
TGGGGGG38350.028.2787366
ACGCAGA73450.027.256487
CGCAGAG75900.026.2895248
GTGGTAT12350.025.6476961
TATCACG1401.0450742E-425.5392912
CGCCTAT1200.001255182324.9016551
GCAGAGT81500.023.2398519
AGAGTAC76900.023.00266310-11
Produced by FastQC (version 0.11.5)