FastQCFastQC Report
Mon 15 Aug 2016
SRR2921340_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921340_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1998341
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18550.057.7365261
GTATCAA35950.055.6112251
TCAACGC44150.043.7978674
CAACGCA45850.042.1739585
GTACATG112400.042.0316351
AACGCAG48550.039.95016
ATCAACG48300.039.9115143
TACATGG117500.039.901452
TATCAAC49400.039.745442
ACATGGG118000.039.3794253
CATGGGG107200.035.9650424
ATGGGGG63700.033.8118935
ACGCAGA58500.032.8475887
CGCAGAG63250.030.3800148
TGGGGGG47450.027.7105756
GCAGAGT72500.026.2577789
AGAGTAC66200.023.76885410-11
ATGGGGA39000.022.1209475
ATGGGGC23800.021.9991595
GAGTACA76650.021.9682961
Produced by FastQC (version 0.11.5)