FastQCFastQC Report
Mon 15 Aug 2016
SRR2921340_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921340_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1998341
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21930.10974103018453808No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20670.10343579999609676No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24150.067.307171
GTATCAA43200.052.7048761
GTACATG108350.043.4065631
TACATGG114150.041.353682
ACATGGG116800.040.00723
CATGGGG104100.037.3303264
ATGGGGG62800.035.1161585
TCAACGC61900.034.8564264
ATCAACG63900.034.045283
TATCAAC65750.033.9032062
CAACGCA64050.033.686385
AACGCAG66100.032.6350986
ACGCAGA80200.026.8873987
TGGGGGG47350.026.3029296
CGCAGAG86850.024.6914867
AGAGTAC85750.023.824310-11
GTGGTAT11400.023.0652081
CAGAGTA91200.022.8583169
ATGGGGA36400.022.7603155
GCAGAGT93600.022.3994758
Produced by FastQC (version 0.11.5)