FastQCFastQC Report
Mon 15 Aug 2016
SRR2921339_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921339_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2655852
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39400.056.7957881
GGTATCA22650.050.449571
TCAACGC48100.045.405954
CAACGCA49150.044.1937755
AACGCAG52250.041.5686236
ATCAACG54600.040.109473
TATCAAC54700.039.8185582
GTACATG129600.038.4365961
TACATGG136450.036.111752
ACATGGG138900.035.0891953
ACGCAGA65550.033.1281667
CATGGGG126300.032.93564
ATGGGGG75300.030.3478555
CGCAGAG73400.029.5840558
GCAGAGT83000.025.8741139
TGGGGGG46600.024.2620076
AGAGTAC73050.022.23159410-11
AGTACAT93250.021.6981052
GTATATG8550.021.578441
GAGTACA95800.021.5570051
Produced by FastQC (version 0.11.5)