FastQCFastQC Report
Mon 15 Aug 2016
SRR2921339_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921339_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2655852
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25100.059.7731171
GTATCAA44600.051.061791
GTACATG123250.039.913411
TACATGG130050.037.808222
TCAACGC56850.037.2169274
ACATGGG134750.036.3568043
TATCAAC62900.035.4375422
CAACGCA59750.035.4105835
CATGGGG121300.034.934254
AACGCAG63100.034.3696756
ATCAACG63450.033.6274573
ATGGGGG69350.030.5087575
ACGCAGA77600.027.635187
CGCAGAG84200.025.1803198
TATACCG2951.7826096E-1024.2438645
TGGGGGG45400.023.2284816
AGAGTAC84700.021.93745610-11
ATGGGGA49750.021.0844465
GCAGAGT98550.020.7281639
GTAAGTA9250.019.9738862
Produced by FastQC (version 0.11.5)