FastQCFastQC Report
Mon 15 Aug 2016
SRR2921338_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921338_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2664436
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41850.058.4453471
GGTATCA23250.051.1930051
TCAACGC51050.045.809934
CAACGCA53550.043.5593385
AACGCAG56650.041.1741336
TATCAAC58050.040.798452
ATCAACG58500.039.772573
GTACATG126800.039.001861
TACATGG133150.037.0489732
ACATGGG135350.035.4795463
ACGCAGA68350.034.2066737
CATGGGG123150.033.534194
CGCAGAG74800.031.0979658
ATGGGGG69100.029.7095225
GCAGAGT88600.025.7837549
AGAGTAC73550.024.42692810-11
ATGGGGA51550.023.0863885
GTATATG7550.022.0706251
CAGAGTA81450.021.5099210-11
TGGGGGG40650.019.90756
Produced by FastQC (version 0.11.5)