##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921338_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2664436
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.692739851886103	31.0	31.0	34.0	28.0	34.0
2	30.854264842540786	31.0	31.0	34.0	28.0	34.0
3	30.936745337474797	33.0	31.0	34.0	28.0	34.0
4	34.45981926381418	37.0	35.0	37.0	32.0	37.0
5	34.47728187128533	37.0	35.0	37.0	32.0	37.0
6	34.69217350313537	37.0	35.0	37.0	32.0	37.0
7	34.70178454277003	37.0	35.0	37.0	32.0	37.0
8	34.694945571970955	37.0	35.0	37.0	32.0	37.0
9	36.385773199281196	39.0	37.0	39.0	32.0	39.0
10-11	36.376644250415474	39.0	37.5	39.0	32.0	39.0
12-13	36.32297322960657	39.0	37.0	39.0	32.0	39.0
14-15	37.695665424127284	41.0	38.0	41.0	32.0	41.0
16-17	37.67639286513169	41.0	38.0	41.0	32.0	41.0
18-19	37.66938819322363	41.0	38.5	41.0	32.0	41.0
20-21	37.69362840766301	41.0	39.0	41.0	32.0	41.0
22-23	37.63165431633561	41.0	38.5	41.0	32.0	41.0
24-25	37.61007188763401	41.0	38.0	41.0	32.0	41.0
26-27	37.56804704635428	41.0	38.0	41.0	32.0	41.0
28-29	37.50065116970346	41.0	38.0	41.0	32.0	41.0
30-31	37.42710408506716	41.0	38.0	41.0	31.5	41.0
32-33	37.35720280014232	41.0	38.0	41.0	31.0	41.0
34-35	37.25678698981698	40.0	38.0	41.0	31.0	41.0
36-37	37.15137237299001	40.0	38.0	41.0	31.0	41.0
38-39	37.061789999834865	40.0	38.0	41.0	30.0	41.0
40-41	36.96514984784773	40.0	38.0	41.0	30.0	41.0
42-43	36.790212825528556	40.0	37.5	41.0	30.0	41.0
44-45	36.56134562811792	40.0	37.0	41.0	30.0	41.0
46-47	36.354071931170424	40.0	36.5	41.0	29.5	41.0
48-49	36.15483370589498	40.0	36.0	41.0	29.0	41.0
50-51	35.98736261632856	40.0	35.5	41.0	29.0	41.0
52-53	35.78821915782552	39.0	35.0	41.0	28.0	41.0
54-55	35.5716095263688	39.0	35.0	41.0	28.0	41.0
56-57	35.320371928618286	39.0	35.0	41.0	28.0	41.0
58-59	35.04301792199175	38.0	35.0	40.0	27.0	41.0
60-61	34.74698566600962	38.0	35.0	40.0	26.5	41.0
62-63	34.42878173842419	37.0	34.0	40.0	26.0	41.0
64-65	34.07026008506115	37.0	34.0	39.5	26.0	41.0
66-67	33.721146801799705	36.0	34.0	39.0	26.0	41.0
68-69	33.35927490846093	35.5	34.0	39.0	25.5	40.0
70-71	33.29194996614668	35.0	34.0	38.5	26.0	40.0
72-73	32.99073875296686	35.0	34.0	37.0	26.0	39.5
74-75	32.66428260990318	35.0	34.0	37.0	25.0	39.0
76-77	32.36443678887389	35.0	33.0	36.5	25.0	39.0
78-79	32.07564546493141	35.0	33.0	36.0	24.5	37.5
80-81	31.800107790166475	35.0	33.0	35.5	24.0	37.0
82-83	31.576930915210575	35.0	33.0	35.0	23.5	37.0
84-85	31.367010879600784	35.0	33.0	35.0	23.0	36.0
86-87	31.2060946481732	35.0	33.0	35.0	21.5	36.0
88-89	31.064273264585825	35.0	33.0	35.0	20.0	36.0
90-91	30.94245198608636	35.0	33.0	35.0	20.0	35.0
92-93	30.832549928014785	35.0	33.0	35.0	19.5	35.0
94-95	30.709409608637625	35.0	32.0	35.0	19.0	35.0
96-97	30.61212673151091	35.0	32.0	35.0	18.0	35.0
98-99	30.530027743207192	35.0	32.0	35.0	17.5	35.0
100-101	30.426992804480946	35.0	32.0	35.0	16.0	35.0
102-103	29.58186216520119	34.0	30.5	35.0	13.0	35.0
104-105	30.174550448950548	34.5	31.5	35.0	11.0	35.0
106-107	30.249759799071924	35.0	32.0	35.0	9.0	35.0
108-109	30.18189046387303	35.0	32.0	35.0	7.0	35.0
110-111	30.0896052673061	35.0	32.0	35.0	4.5	35.0
112-113	29.988499817597422	35.0	32.0	35.0	2.0	35.0
114-115	29.85932125973377	35.0	31.0	35.0	2.0	35.0
116-117	29.72130668554246	34.5	31.0	35.0	2.0	35.0
118-119	29.715981543561192	34.5	31.5	35.0	2.0	35.0
120-121	29.884243044306565	35.0	32.0	35.0	2.0	35.0
122-123	29.746065771517873	35.0	32.0	35.0	2.0	35.0
124-125	29.171408508217123	34.5	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	69707.0
3	10197.0
4	6703.0
5	5808.0
6	6445.0
7	7236.0
8	7497.0
9	7922.0
10	8467.0
11	8985.0
12	9067.0
13	8855.0
14	8740.0
15	7866.0
16	7022.0
17	6908.0
18	6622.0
19	6445.0
20	6625.0
21	6967.0
22	7919.0
23	10002.0
24	13605.0
25	17488.0
26	19854.0
27	22843.0
28	27649.0
29	33392.0
30	40811.0
31	51183.0
32	66541.0
33	90749.0
34	138181.0
35	237414.0
36	528010.0
37	906610.0
38	238040.0
39	61.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.93135803433074	17.484556871554013	12.117600511717859	25.466484582397385
2	16.268828541796555	19.416975369232542	39.113031120747024	25.20116496822387
3	19.276649860515956	24.175970607754053	28.40196929249008	28.145410239239908
4	13.46056322537611	15.497537770513981	35.32299893883334	35.71890006527658
5	14.91743087585279	37.23196376237148	33.21584930815797	14.634756053617757
6	33.98251690755601	34.31150749999437	17.349100954271695	14.356874638177928
7	29.985154321856555	31.2956654748834	20.52457231872226	18.194607884537785
8	27.336590236422637	34.61454870996984	19.396715945624855	18.652145107982665
9	27.898904175917394	15.425126820195894	18.524062525218593	38.15190647866812
10-11	26.47898325232465	25.062189860035982	26.920331856916498	21.53849503072287
12-13	26.620327232374848	22.549093019864664	27.041645999526697	23.78893374823379
14-15	24.163991133017497	23.64005696127939	24.037558562095526	28.158393343607585
16-17	24.538205345368485	26.631995980942886	24.617494271023247	24.21230440266538
18-19	24.62433844362041	26.2171220484137	25.733668159697988	23.4248713482679
20-21	25.577166719870405	25.162365233618832	25.333311563902754	23.92715648260801
22-23	25.522457244626608	24.846197009704294	25.17707377401442	24.454271971654684
24-25	24.856978030804616	25.261590766121827	25.18113738966984	24.700293813403718
26-27	24.663509997817442	25.343724530192002	25.371311446336975	24.62145402565358
28-29	24.78045589206203	25.4205934756637	25.33618014083402	24.462770491440246
30-31	25.045110576508943	25.356225766260497	25.04845105809754	24.550212599133015
32-33	24.744536547474475	25.39859129845004	25.125660299527837	24.731211854547652
34-35	24.89682740759508	25.384042263697697	25.31163846897315	24.40749185973407
36-37	24.981406569772517	25.33105501647276	25.145937079794578	24.541601333960152
38-39	24.894120817591663	25.507748083458715	25.143779303624918	24.45435179532471
40-41	24.94082722525666	25.28379660630155	25.244573295418714	24.53080287302307
42-43	24.728342108498005	25.216773623271905	25.210993368659196	24.84389089957089
44-45	24.72642322791127	25.268960076089154	25.31248088106077	24.692135814938805
46-47	24.892274869491192	25.23147765848356	25.20330772452995	24.672939747495295
48-49	24.668401013132588	25.412035528704173	25.15738237047202	24.76218108769122
50-51	24.60528750533446	25.43964018810328	25.243245738719	24.711826567843257
52-53	24.75423022037457	25.3546358831385	25.228654474263614	24.662479422223324
54-55	24.609471326699627	25.179364126724252	25.337305899207774	24.873858647368344
56-57	24.623480451723992	25.32553873293954	25.22631709823167	24.8246637171048
58-59	24.737378546668126	25.290950320419288	25.24099242430356	24.73067870860903
60-61	24.854215518754042	25.275509351919883	25.249573761139217	24.620701368186864
62-63	24.746948328965672	25.37116997550556	25.319542480176615	24.562339215352154
64-65	24.663025354380505	25.119687576531835	25.313845024973425	24.90344204411424
66-67	24.65600464509378	25.38823747103619	25.143634399291525	24.812123484578503
68-69	24.581483147568772	25.244275191662073	25.208899508475056	24.9653421522941
70-71	24.54566882645767	25.03696091395079	25.46330163145981	24.954068628131733
72-73	24.607201522794913	25.277205762164073	25.204147488009564	24.911445227031447
74-75	24.39782511232222	25.373326755048144	25.316314115995386	24.912534016634254
76-77	24.46731427758566	25.058669135352535	25.357527505760878	25.116489081300923
78-79	24.432185405905706	25.049374366165257	25.380375913266263	25.138064314662778
80-81	24.55034847266172	25.28563348310145	25.24074433415242	24.923273710084406
82-83	24.576509428792555	25.15820373638566	25.390930252983352	24.874356581838438
84-85	24.506772795313246	25.176019860460297	25.27852764144272	25.03867970278374
86-87	24.322735721299217	25.25288561725485	25.48730448516805	24.937074176277886
88-89	24.382567422049853	25.049478305550544	25.472629010482684	25.095325261916923
90-91	24.446733567529517	24.99611059679866	25.476050753474517	25.081105082197308
92-93	24.458264645208946	25.16303107555844	25.460229200635382	24.91847507859723
94-95	24.460458522520195	24.95671394571011	25.528187450666284	25.054640081103408
96-97	24.314238098634107	24.98376617283302	25.564730743678194	25.137264984854685
98-99	24.31112909406809	24.956432408207785	25.455446953008558	25.27699154471557
100-101	24.273383393116433	24.966064106324772	25.62000919970971	25.14054330084909
102-103	24.12995064160657	24.937520842806627	25.64517693935923	25.287351576227575
104-105	24.10970534292209	25.041113042616626	25.57639425432679	25.27278736013449
106-107	24.126294153999986	24.872247831980633	25.69539048745787	25.30606752656151
108-109	24.083147571306156	24.903303029631967	25.690297197974214	25.32325220108766
110-111	24.020472695409538	25.117899852171256	25.58675925872057	25.274868193698634
112-113	23.95342543802526	25.187320284939275	25.660110941501422	25.199143335534046
114-115	23.980211408841175	25.221239519307332	25.606115682038133	25.192433389813363
116-117	23.795124649913564	25.110759054205506	25.732619911407774	25.361496384473163
118-119	23.84835792506609	25.00541766789367	25.73786005407331	25.408364352966938
120-121	23.692888166036507	25.065565321903488	25.872787322874164	25.36875918918584
122-123	23.708008929670587	25.21414019642873	25.737358390857747	25.34049248304293
124-125	23.642992834927853	25.129040170080618	25.760874889069086	25.46709210592244
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	363.0
1	296.5
2	215.0
3	179.0
4	137.5
5	117.5
6	111.5
7	136.0
8	186.0
9	201.5
10	203.5
11	203.0
12	214.0
13	249.5
14	274.5
15	310.5
16	356.5
17	369.5
18	410.0
19	472.5
20	551.5
21	623.5
22	684.0
23	875.5
24	1062.0
25	1276.0
26	1722.5
27	2382.0
28	3158.5
29	4547.5
30	6727.0
31	9558.5
32	13536.5
33	18166.0
34	23338.5
35	29333.0
36	36509.5
37	43708.0
38	49589.5
39	55744.5
40	62017.0
41	67338.0
42	72852.5
43	80832.5
44	89565.5
45	97026.5
46	103557.0
47	108637.0
48	114340.0
49	119234.5
50	120489.0
51	121277.5
52	120613.0
53	119396.5
54	119732.5
55	118137.5
56	111498.5
57	101518.5
58	93350.0
59	84763.0
60	72824.5
61	61058.5
62	49947.5
63	37978.0
64	27636.5
65	20357.0
66	15250.5
67	11563.5
68	8695.0
69	6421.5
70	4873.5
71	3690.5
72	2739.0
73	2133.5
74	1632.5
75	1130.5
76	773.0
77	496.0
78	319.0
79	219.0
80	146.5
81	101.0
82	71.5
83	54.0
84	39.0
85	31.5
86	25.5
87	27.0
88	26.0
89	17.5
90	11.5
91	9.0
92	9.5
93	6.5
94	4.0
95	3.5
96	3.5
97	4.0
98	1.5
99	2.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01756469286558206
2	0.01234782895892414
3	0.012460423143959924
4	0.014524649869615934
5	0.009157660382910304
6	0.03130118343994752
7	0.01354883359930582
8	0.013173519649186543
9	0.008669752247755248
10-11	0.008088015625070371
12-13	0.006849479589676765
14-15	0.0063803371520276715
16-17	0.004860315654044608
18-19	0.006361571454521707
20-21	0.005610943554283158
22-23	0.005667240646801049
24-25	0.006005023201908396
26-27	0.004860315654044608
28-29	0.006192680176968034
30-31	0.005610943554283158
32-33	0.007862827254998806
34-35	0.007919124347516699
36-37	0.00707466795974833
38-39	0.007374919119843749
40-41	0.007656404582433204
42-43	0.007262324934807967
44-45	0.007243559237302003
46-47	0.009307785962958014
48-49	0.008407032482671755
50-51	0.0058173662268487586
52-53	0.005779834831836831
54-55	0.0061176173869441795
56-57	0.0074499819098676045
58-59	0.00722479353979604
60-61	0.005385755184211593
62-63	0.005929960411884541
64-65	0.0038844993837344934
66-67	0.0044850017039253336
68-69	0.0066242912196051995
70-71	0.00506673832661021
72-73	0.004672658678984971
74-75	0.004297344728865696
76-77	0.004897847049056535
78-79	0.0036780767111688926
80-81	0.00356548252613311
82-83	0.005629709251789122
84-85	0.009307785962958014
86-87	0.007055902262242365
88-89	0.005141801116634065
90-91	0.005460817974235448
92-93	0.008256906902624046
94-95	0.006868245287182728
96-97	0.00923272317293416
98-99	0.006943308077206584
100-101	0.008313203995141936
102-103	0.005535880764259303
104-105	0.005085504024116174
106-107	0.007337387724831822
108-109	0.006943308077206584
110-111	0.007675170279939169
112-113	0.005592177856777194
114-115	0.002721026138364742
116-117	0.0035279511311211826
118-119	0.1560743061571004
120-121	0.011221887108566315
122-123	0.002251883700715649
124-125	0.0019703982381261928
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2664436.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.324152049634776
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.35948303213755	41.48164166669346
2	12.313626981531138	13.378546888543655
3	4.317587394253841	7.036478222790961
4	2.04222482495687	4.43768527641983
5	1.171477613877504	3.1819764009512426
6	0.7522768576177534	2.4520081437988974
7	0.5443746131058007	2.070088247802443
8	0.3968444166617405	1.7246589144624804
9	0.29846037861007507	1.4592246289564752
>10	1.6743595562978837	16.55494436280169
>50	0.10026477578919621	3.7206755167722823
>100	0.02888127940018738	2.463048378682433
>500	1.3827576054611674E-4	0.03902335132421545
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	7.506279002385495E-5	0.0	0.0	0.0	3.7531395011927475E-5
7	7.506279002385495E-5	0.0	0.0	0.0	3.7531395011927475E-5
8	7.506279002385495E-5	0.0	0.0	0.0	1.1259418503578243E-4
9	7.506279002385495E-5	0.0	0.0	0.0	1.501255800477099E-4
10-11	7.506279002385495E-5	0.0	0.0	0.0	1.501255800477099E-4
12-13	7.506279002385495E-5	0.0	0.0	0.0	1.8765697505963738E-4
14-15	7.506279002385495E-5	0.0	0.0	0.0	2.439540675775286E-4
16-17	1.1259418503578243E-4	0.0	0.0	3.7531395011927475E-5	3.377825551073473E-4
18-19	1.501255800477099E-4	0.0	0.0	3.7531395011927475E-5	3.377825551073473E-4
20-21	1.501255800477099E-4	0.0	0.0	3.7531395011927475E-5	4.3161104263716597E-4
22-23	1.501255800477099E-4	0.0	0.0	1.501255800477099E-4	6.192680176968033E-4
24-25	1.501255800477099E-4	0.0	0.0	3.5654825261331106E-4	7.506279002385495E-4
26-27	1.501255800477099E-4	0.0	0.0	6.943308077206583E-4	7.506279002385495E-4
28-29	1.501255800477099E-4	0.0	0.0	0.002533369163305105	7.506279002385495E-4
30-31	1.501255800477099E-4	0.0	0.0	0.010602619090869512	7.88159295250477E-4
32-33	1.501255800477099E-4	0.0	0.0	0.029631036361916743	7.88159295250477E-4
34-35	1.8765697505963738E-4	0.0	0.0	0.052543953016698466	7.88159295250477E-4
36-37	1.8765697505963738E-4	0.0	0.0	0.08735432189026121	7.88159295250477E-4
38-39	1.8765697505963738E-4	0.0	0.0	0.14659762891658873	8.256906902624045E-4
40-41	1.8765697505963738E-4	0.0	0.0	0.212690415532593	8.256906902624045E-4
42-43	1.8765697505963738E-4	0.0	0.0	0.27902715621617485	8.632220852743319E-4
44-45	1.8765697505963738E-4	0.0	0.0	0.35249486195202284	8.632220852743319E-4
46-47	1.8765697505963738E-4	0.0	0.0	0.43590838736603166	9.195191777922232E-4
48-49	1.8765697505963738E-4	0.0	0.0	0.5251205133093833	0.001013347665322042
50-51	2.814854625894561E-4	0.0	0.0	0.616922305508558	0.001013347665322042
52-53	3.377825551073473E-4	0.0	0.0	0.7081986581775654	0.0011634732453697518
54-55	3.377825551073473E-4	0.0	0.0	0.805799050906083	0.0012760674304055342
56-57	3.377825551073473E-4	0.0	0.0	0.9107180656619263	0.001369895917935353
58-59	3.377825551073473E-4	0.0	0.0	1.0250574605657632	0.0013886616154413166
60-61	3.377825551073473E-4	0.0	0.0	1.1497367547953863	0.0013886616154413166
62-63	3.377825551073473E-4	0.0	0.0	1.2811529344296504	0.001426193010453244
64-65	3.377825551073473E-4	0.0	0.0	1.4115932977936043	0.0014449587079592078
66-67	3.377825551073473E-4	0.0	0.0	1.5550382895291912	0.0014824901029711355
68-69	3.377825551073473E-4	0.0	0.0	1.7188440630587487	0.001501255800477099
70-71	3.377825551073473E-4	0.0	0.0	1.8995389643436735	0.0015200214979830629
72-73	3.377825551073473E-4	0.0	0.0	2.077606667977763	0.0017264441705486639
74-75	3.377825551073473E-4	0.0	0.0	2.2605722186609096	0.0017264441705486639
76-77	3.377825551073473E-4	0.0	0.0	2.464236333693134	0.0017264441705486639
78-79	3.377825551073473E-4	0.0	0.0	2.690925959565176	0.0017264441705486639
80-81	3.377825551073473E-4	0.0	0.0	2.926942137097682	0.0017452098680546277
82-83	3.5654825261331106E-4	0.0	0.0	3.1794533627379304	0.0017639755655605915
84-85	3.7531395011927476E-4	0.0	0.0	3.435173522651698	0.0017639755655605915
86-87	3.7531395011927476E-4	0.0	0.0	3.7130747370175152	0.001782741263066555
88-89	4.1284534513120227E-4	0.0	0.0	4.016910145336574	0.001801506960572519
90-91	4.1284534513120227E-4	0.0	0.0	4.351371171985366	0.001801506960572519
92-93	4.1284534513120227E-4	0.0	0.0	4.698968186888332	0.001801506960572519
94-95	4.1284534513120227E-4	0.0	0.0	5.059757487137992	0.0021017581206679388
96-97	4.1284534513120227E-4	0.0	0.0	5.4303049500907505	0.0021392895156798664
98-99	4.503767401431297E-4	0.0	0.0	5.83033332382538	0.00215805521318583
100-101	4.503767401431297E-4	0.0	0.0	6.266035288518847	0.0021768209106917936
102-103	4.879081351550572E-4	0.0	0.0	6.726432911130161	0.002233118003209685
104-105	5.442052276729485E-4	0.0	0.0	7.2082046632007675	0.0022706493982216124
106-107	5.629709251789122E-4	0.0	0.0	7.703337591895621	0.002345712188245467
108-109	5.629709251789122E-4	0.0	0.0	8.223747915131007	0.0025146034657991412
110-111	5.629709251789122E-4	0.0	0.0	8.781483210705755	0.0026834947433528144
112-113	5.629709251789122E-4	0.0	0.0	9.370838706578052	0.0027397918358707057
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4185	0.0	58.445347	1
GGTATCA	2325	0.0	51.193005	1
TCAACGC	5105	0.0	45.80993	4
CAACGCA	5355	0.0	43.559338	5
AACGCAG	5665	0.0	41.174133	6
TATCAAC	5805	0.0	40.79845	2
ATCAACG	5850	0.0	39.77257	3
GTACATG	12680	0.0	39.00186	1
TACATGG	13315	0.0	37.048973	2
ACATGGG	13535	0.0	35.479546	3
ACGCAGA	6835	0.0	34.206673	7
CATGGGG	12315	0.0	33.53419	4
CGCAGAG	7480	0.0	31.097965	8
ATGGGGG	6910	0.0	29.709522	5
GCAGAGT	8860	0.0	25.783754	9
AGAGTAC	7355	0.0	24.426928	10-11
ATGGGGA	5155	0.0	23.086388	5
GTATATG	755	0.0	22.070625	1
CAGAGTA	8145	0.0	21.50992	10-11
TGGGGGG	4065	0.0	19.9075	6
>>END_MODULE