FastQCFastQC Report
Mon 15 Aug 2016
SRR2921338_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921338_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2664436
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25100.058.567921
GTATCAA43500.051.3784181
GTACATG121200.039.4444351
TACATGG130950.037.0038532
ACATGGG134100.035.9568523
TCAACGC60450.034.8048974
CATGGGG116400.034.7167174
CAACGCA62750.033.624165
TATCAAC65800.033.3337252
ATCAACG65350.032.4688073
AACGCAG66000.031.5965066
ATGGGGG66000.028.6270925
ACGCAGA79750.025.5444227
CGCAGAG85150.024.1997328
ATGGGGC36600.021.98445
AGAGTAC85000.021.6838510-11
ATGGGGA50700.021.1605265
TGGGGGG41650.020.8858816
GGACCGT4850.020.8844056
CAGAGTA91650.019.9491799
Produced by FastQC (version 0.11.5)