FastQCFastQC Report
Mon 15 Aug 2016
SRR2921337_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921337_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2100258
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28650.054.209261
GGTATCA16900.053.167691
GTACATG101200.041.5127751
TCAACGC36300.040.6524
CAACGCA37950.039.197175
TACATGG105850.039.125082
ACATGGG105500.038.634473
TATCAAC41050.036.6728132
AACGCAG41100.036.4799466
ATCAACG41900.035.2187963
CATGGGG100050.034.613284
ATGGGGG59750.031.2693085
ACGCAGA52050.028.6884547
CGCAGAG57750.025.6508338
TGGGGGG41850.023.7419576
ATGGGGA36450.023.6699315
GCAGAGT67350.021.9935469
AGAGTAC58200.021.5160510-11
GTATATG6950.020.5486831
GAGTACA68800.020.152321
Produced by FastQC (version 0.11.5)