##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921337_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2100258
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.381105559412223	31.0	31.0	34.0	27.0	34.0
2	30.427082291794626	31.0	31.0	34.0	27.0	34.0
3	30.61369460323446	33.0	31.0	34.0	27.0	34.0
4	34.11547866976343	37.0	35.0	37.0	30.0	37.0
5	34.0987397738754	37.0	35.0	37.0	30.0	37.0
6	34.170247179156085	37.0	35.0	37.0	30.0	37.0
7	34.04194198998409	37.0	35.0	37.0	30.0	37.0
8	34.028817888088035	37.0	35.0	37.0	30.0	37.0
9	35.64419418947577	39.0	37.0	39.0	30.0	39.0
10-11	35.689576947213155	39.0	37.0	39.0	30.0	39.0
12-13	35.65428390226344	39.0	37.0	39.0	30.0	39.0
14-15	36.94572119234875	40.0	38.0	41.0	30.0	41.0
16-17	36.909337090966915	40.0	38.0	41.0	30.0	41.0
18-19	36.874635640002325	40.0	38.0	41.0	30.0	41.0
20-21	36.91770987183479	40.0	38.0	41.0	30.0	41.0
22-23	36.83050868036213	40.0	38.0	41.0	30.0	41.0
24-25	36.79863188236874	40.0	38.0	41.0	30.0	41.0
26-27	36.76379163893198	40.0	38.0	41.0	30.0	41.0
28-29	36.692679423194676	40.0	38.0	41.0	30.0	41.0
30-31	36.61664828797224	40.0	38.0	41.0	30.0	41.0
32-33	36.54713968474349	40.0	38.0	41.0	30.0	41.0
34-35	36.43026832893864	40.0	37.0	41.0	29.0	41.0
36-37	36.31245637440733	40.0	37.0	41.0	29.0	41.0
38-39	36.211369507936645	40.0	37.0	41.0	28.0	41.0
40-41	36.114933736712345	40.0	37.0	41.0	28.0	41.0
42-43	35.89868292371699	40.0	36.0	41.0	27.0	41.0
44-45	35.60201246703976	40.0	35.5	41.0	26.5	41.0
46-47	35.3621624105229	39.5	35.0	41.0	26.0	41.0
48-49	35.143469040470265	39.0	35.0	41.0	24.5	41.0
50-51	34.95815133188398	39.0	35.0	41.0	24.0	41.0
52-53	34.75338053705783	39.0	35.0	40.0	24.0	41.0
54-55	34.541374678729944	38.5	34.5	40.0	23.5	41.0
56-57	34.28406938576117	38.0	34.0	40.0	23.0	41.0
58-59	34.00752740853743	38.0	34.0	40.0	22.5	41.0
60-61	33.7078918399549	37.0	34.0	40.0	22.0	41.0
62-63	33.38635181963359	37.0	33.0	40.0	21.5	41.0
64-65	33.0327943043188	36.0	33.0	39.0	20.0	41.0
66-67	32.69268799357032	36.0	33.0	39.0	20.0	40.0
68-69	32.34149304514017	35.0	32.5	38.5	18.5	40.0
70-71	32.31532126053085	35.0	33.0	38.0	20.0	40.0
72-73	32.03240030510537	35.0	33.0	37.0	18.5	39.0
74-75	31.71595346857386	35.0	33.0	37.0	18.0	39.0
76-77	31.421288003664312	35.0	32.0	36.0	17.5	39.0
78-79	31.143087182622324	35.0	32.0	36.0	15.5	37.0
80-81	30.87292442166629	35.0	32.0	35.0	11.5	37.0
82-83	30.657640632722263	35.0	32.0	35.0	10.5	36.5
84-85	30.44710745060845	35.0	32.0	35.0	7.5	36.0
86-87	30.283574446567993	35.0	31.5	35.0	7.0	36.0
88-89	30.140948159702283	35.0	31.0	35.0	6.5	35.5
90-91	30.02085434265695	35.0	31.0	35.0	2.0	35.0
92-93	29.908372209509498	34.5	31.0	35.0	2.0	35.0
94-95	29.782372213318553	34.0	31.0	35.0	2.0	35.0
96-97	29.690191633599298	34.0	31.0	35.0	2.0	35.0
98-99	29.60384533709668	34.0	31.0	35.0	2.0	35.0
100-101	29.497788366953014	34.0	31.0	35.0	2.0	35.0
102-103	28.591572559180825	33.5	29.0	34.5	2.0	35.0
104-105	29.225906055351295	34.0	30.0	35.0	2.0	35.0
106-107	29.317971649197382	34.0	31.0	35.0	2.0	35.0
108-109	29.253667406575765	34.0	31.0	35.0	2.0	35.0
110-111	29.158827153616365	34.0	30.0	35.0	2.0	35.0
112-113	29.058141428338807	34.0	30.0	35.0	2.0	35.0
114-115	28.92492422359539	34.0	30.0	35.0	2.0	35.0
116-117	28.78367919560359	34.0	30.0	35.0	2.0	35.0
118-119	28.783149260709877	34.0	30.0	35.0	2.0	35.0
120-121	28.969192832499626	34.0	31.0	35.0	2.0	35.0
122-123	28.822723446357543	34.0	31.0	35.0	2.0	35.0
124-125	28.196560851095438	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	77755.0
3	10992.0
4	6926.0
5	5866.0
6	6104.0
7	6682.0
8	7026.0
9	7565.0
10	8142.0
11	8508.0
12	8177.0
13	8017.0
14	7777.0
15	7127.0
16	6481.0
17	6134.0
18	5849.0
19	5863.0
20	5791.0
21	6193.0
22	7149.0
23	8862.0
24	11655.0
25	14842.0
26	17035.0
27	20219.0
28	24747.0
29	29799.0
30	36717.0
31	46395.0
32	59765.0
33	82640.0
34	127853.0
35	211810.0
36	432448.0
37	625066.0
38	130267.0
39	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.01849358807991	17.297159840127012	12.261675104301743	25.422671467491337
2	16.17751678779146	19.227318743756065	39.32581219958877	25.269352268863702
3	19.41270698395556	24.083042148680835	28.317576505612745	28.186674361750857
4	13.506794063795391	15.039226283872104	35.032416743849396	36.42156290848311
5	14.693783483540537	37.60327489323122	32.7680266501722	14.934914973056031
6	34.16150902418754	33.97028531149462	17.35625477761611	14.511950886701735
7	30.399757330328853	31.200100572673755	20.222583708179247	18.177558388818145
8	27.508598862754862	35.25069440906063	19.048165536262843	18.192541191921666
9	28.281104915550433	15.625145828472384	18.134871694753034	37.95887756122415
10-11	26.78899943169143	25.132976348887652	26.64593423667493	21.43208998274599
12-13	26.68329432357411	22.329605205047056	27.022394720671315	23.96470575070752
14-15	24.210476694753844	23.59944251017081	23.914494735558975	28.27558605951637
16-17	24.74754869575258	26.54262835049276	24.475307209102173	24.234515744652484
18-19	24.78420932463322	26.214552416034188	25.531331341865044	23.46990691746755
20-21	25.62604129913182	25.21121200909844	25.183594815776654	23.97915187599309
22-23	25.57749748945189	24.726982356882093	25.09421995401257	24.60130019965345
24-25	24.95340773493501	25.078328335239565	25.14908572500612	24.819178204819313
26-27	24.733972903042716	25.197341919337653	25.259860905968207	24.808824271651424
28-29	24.827296240991025	25.569179592862913	25.075733479102226	24.527790687043836
30-31	25.289403532195614	25.31837781174908	24.887168155814486	24.50505050024082
32-33	24.982988816188268	25.324807662913333	25.03215339921308	24.66005012168532
34-35	25.011808972724133	25.489000989477635	25.15261192572918	24.346578112069057
36-37	25.02055847630621	25.448560478108973	25.09348333203975	24.437397713545074
38-39	25.067044234910046	25.488094410472613	25.00321412347758	24.44164723113976
40-41	25.050854721203752	25.273272701299938	25.057616303985526	24.618256273510784
42-43	24.788516375502446	25.29803888236937	25.04583702660369	24.8676077155245
44-45	24.688551286721378	25.261736913154344	25.301044471585943	24.748667328538335
46-47	24.745536971690562	25.1620489800408	25.239499166571232	24.852914881697398
48-49	24.673352273850064	25.327897676449528	25.203688329535467	24.795061720164938
50-51	24.62548285528924	25.446952878085366	25.230419436822586	24.697144829802813
52-53	24.80009694577941	25.275136026451534	25.22306814345499	24.70169888431407
54-55	24.524772900692934	25.0590023700942	25.46154935831277	24.9546753709001
56-57	24.59778067499707	25.268416045346452	25.188443586918325	24.945359692738148
58-59	24.750714607807332	25.27964104617726	25.245904605631246	24.72373974038416
60-61	24.998071564480217	25.20108106666768	25.279646958214375	24.521200410637725
62-63	24.872091434209167	25.253805354232327	25.27354219947022	24.60056101208828
64-65	24.74672421091325	25.00808263668703	25.3084853162656	24.936707836134122
66-67	24.741057046252855	25.299701500781126	25.15828457941322	24.8009568735528
68-69	24.588100427714163	25.20944661429658	25.268990901699546	24.933462056289706
70-71	24.56845477944144	25.003208094253925	25.448436818875166	24.97990030742947
72-73	24.61752473670565	25.383701355391246	25.113961094074117	24.884812813828987
74-75	24.44486134121776	25.403496425414378	25.293291244747163	24.858350988620703
76-77	24.514346525978972	24.960764894103306	25.323784854487275	25.201103725430446
78-79	24.39458496998964	25.05214405448265	25.444179733133858	25.10909124239385
80-81	24.603286082075392	25.2275388286113	25.196327381652427	24.972847707660875
82-83	24.601509373049986	25.267654329335116	25.308508680478266	24.82232761713664
84-85	24.59313652779838	25.327425246760864	25.092669993397802	24.986768232042948
86-87	24.325474844853144	25.435323057348985	25.307401003853613	24.931801093944262
88-89	24.299086780820254	25.120622164194707	25.394149854796293	25.18614120018875
90-91	24.526740447374404	24.998779844701012	25.41856088336863	25.05591882455595
92-93	24.613664538896156	25.109906239531675	25.39006149987703	24.88636772169514
94-95	24.599788344168097	25.04781302861877	25.328965302966633	25.023433324246497
96-97	24.322417039732024	25.0023035094532	25.531479748282965	25.143799702531812
98-99	24.227241754824874	25.089912085431838	25.323328763724895	25.359517396018393
100-101	24.26169549569969	25.050855157084346	25.500813777747727	25.18663556946824
102-103	24.114887442161663	24.99966073730711	25.57226473917841	25.31318708135281
104-105	24.24308508878042	25.037669865559824	25.458758133631658	25.260486912028096
106-107	24.198442931288984	24.921479929527166	25.590519499071473	25.289557640112374
108-109	24.19553561161245	24.88726177718913	25.613748051588765	25.303454559609655
110-111	24.04216101008908	25.220157021692387	25.571332113069335	25.1663498551492
112-113	24.031798764323124	25.265951938069026	25.55950316752014	25.14274613008772
114-115	24.075074415195246	25.31240335785549	25.503598569571274	25.10892365737799
116-117	23.909623501089424	25.206171626213226	25.62484763297832	25.259357239719034
118-119	23.931439710758895	25.03070945517421	25.67317605387316	25.364674780193734
120-121	23.815265232304156	25.0983915921081	25.833048813181925	25.253294362405814
122-123	23.79920174934263	25.24388545472076	25.668151036737864	25.288761759198753
124-125	23.75505808058504	25.169951745726713	25.663445667935658	25.411544505752587
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	320.0
1	255.0
2	172.0
3	142.5
4	122.5
5	110.0
6	114.0
7	134.0
8	167.0
9	173.0
10	172.0
11	187.5
12	210.5
13	245.5
14	270.0
15	304.0
16	334.0
17	351.5
18	361.0
19	388.5
20	434.5
21	488.5
22	571.5
23	702.5
24	844.5
25	1039.5
26	1397.5
27	1870.0
28	2562.5
29	3688.0
30	5390.5
31	7498.0
32	10243.5
33	13380.0
34	16762.0
35	20959.0
36	26041.5
37	31205.0
38	36202.0
39	41712.0
40	47049.0
41	52122.5
42	57468.0
43	63756.0
44	70436.0
45	76270.0
46	81538.5
47	86693.5
48	92599.5
49	97037.0
50	98221.5
51	98976.5
52	98391.5
53	97064.0
54	96427.0
55	93412.5
56	87792.0
57	80698.5
58	73778.0
59	66237.0
60	56544.5
61	47134.5
62	38558.5
63	29544.5
64	21569.5
65	16076.0
66	12120.0
67	9060.0
68	6779.0
69	5048.5
70	3883.5
71	2930.5
72	2090.5
73	1579.0
74	1201.0
75	880.5
76	607.0
77	380.0
78	245.5
79	168.5
80	125.5
81	90.5
82	62.5
83	48.0
84	37.0
85	25.0
86	21.0
87	22.0
88	17.5
89	14.0
90	10.5
91	7.5
92	7.0
93	5.5
94	3.0
95	3.0
96	2.5
97	2.5
98	4.5
99	3.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016236100517174556
2	0.010474903559467456
3	0.010284450767477138
4	0.012522271073363367
5	0.007570498481615116
6	0.029663022352491932
7	0.013522148231312535
8	0.012903176657344003
9	0.008951281223544917
10-11	0.00792759746659696
12-13	0.006784880714655057
14-15	0.006189715739685316
16-17	0.004785126398756724
18-19	0.006308748734679263
20-21	0.005570744165716784
22-23	0.005665970561711942
24-25	0.005904036551699838
26-27	0.0045708670077676174
28-29	0.006189715739685316
30-31	0.005689777160710732
32-33	0.008046630461590909
34-35	0.007618111679612695
36-37	0.007189592897634481
38-39	0.00723720609563206
40-41	0.007522885283617537
42-43	0.007356239090626009
44-45	0.007284819293629639
46-47	0.0094512198025195
48-49	0.008308503050577596
50-51	0.005737390358708311
52-53	0.005475517769721625
54-55	0.006213522338684105
56-57	0.007618111679612695
58-59	0.007380045689624798
60-61	0.005237451779733728
62-63	0.0059516497496974186
64-65	0.00366621624581361
66-67	0.0040471218297942445
68-69	0.006689654318659898
70-71	0.004832739596754304
72-73	0.0042613812207833515
74-75	0.004023315230795454
76-77	0.0046660934037627754
78-79	0.003166277666839026
80-81	0.0031900842658378163
82-83	0.005451711170722835
84-85	0.0094512198025195
86-87	0.007189592897634481
88-89	0.0049993857897458315
90-91	0.005499324368720414
92-93	0.008213276654582437
94-95	0.007165786298635692
96-97	0.009594059396512238
98-99	0.007189592897634481
100-101	0.008379922847573964
102-103	0.005499324368720414
104-105	0.004975579190747041
106-107	0.007522885283617537
108-109	0.007118173100638113
110-111	0.007903790867598171
112-113	0.0054040979727252554
114-115	0.0023092401028825984
116-117	0.003142471067840237
118-119	0.15160042242429264
120-121	0.010998648737440829
122-123	0.0019283345189019636
124-125	0.0014045893409285906
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2100258.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.63602277882117
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.76676086509016	47.56972367943299
2	10.636614559786938	12.686507763539897
3	3.6458751379945107	6.522764783345354
4	1.7304517315645398	4.127890355249337
5	1.0446717632279743	3.1150034534127373
6	0.6606497900616628	2.363911555736445
7	0.46145984343877205	1.9263740814378105
8	0.3330234535809381	1.5888155410907618
9	0.2605781121029395	1.398585800612981
>10	1.3786077647832953	14.537333919488047
>50	0.06709805421360948	2.7359648800653265
>100	0.014049441836869177	1.3310641860868493
>500	7.974115891362332E-5	0.03604627741591889
>1k	7.974115891362332E-5	0.06001372308560639
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	4.7613197997579344E-5
7	0.0	0.0	0.0	0.0	4.7613197997579344E-5
8	0.0	0.0	0.0	0.0	4.7613197997579344E-5
9	0.0	0.0	0.0	0.0	4.7613197997579344E-5
10-11	4.7613197997579344E-5	0.0	0.0	0.0	4.7613197997579344E-5
12-13	4.7613197997579344E-5	0.0	0.0	0.0	9.522639599515869E-5
14-15	4.7613197997579344E-5	0.0	0.0	0.0	1.4283959399273804E-4
16-17	4.7613197997579344E-5	0.0	0.0	4.7613197997579344E-5	1.9045279199031738E-4
18-19	4.7613197997579344E-5	0.0	0.0	9.522639599515869E-5	1.9045279199031738E-4
20-21	4.7613197997579344E-5	0.0	0.0	9.522639599515869E-5	2.856791879854761E-4
22-23	4.7613197997579344E-5	0.0	0.0	1.666461929915277E-4	3.0948578698426575E-4
24-25	7.141979699636902E-5	0.0	0.0	3.332923859830554E-4	3.332923859830554E-4
26-27	9.522639599515869E-5	0.0	0.0	3.8090558398063475E-4	3.332923859830554E-4
28-29	9.522639599515869E-5	0.0	0.0	0.0013807827419298011	3.332923859830554E-4
30-31	9.522639599515869E-5	0.0	0.0	0.007356239090626009	3.570989849818451E-4
32-33	1.1903299499394837E-4	0.0	0.0	0.022521042652855032	5.237451779733727E-4
34-35	1.4283959399273804E-4	0.0	0.0	0.04073309088692913	5.713583759709522E-4
36-37	1.4283959399273804E-4	0.0	0.0	0.0698723680614477	5.713583759709522E-4
38-39	1.4283959399273804E-4	0.0	0.0	0.11698562748005245	5.713583759709522E-4
40-41	1.4283959399273804E-4	0.0	0.0	0.17464521025512103	6.189715739685315E-4
42-43	1.4283959399273804E-4	0.0	0.0	0.22920993516034696	6.189715739685315E-4
44-45	1.4283959399273804E-4	0.0	0.0	0.28413175905055477	6.189715739685315E-4
46-47	1.4283959399273804E-4	0.0	0.0	0.34595749665041153	6.189715739685315E-4
48-49	1.4283959399273804E-4	0.0	0.0	0.4144490819699294	7.141979699636902E-4
50-51	1.4283959399273804E-4	0.0	0.0	0.4897017414051036	7.141979699636902E-4
52-53	1.4283959399273804E-4	0.0	0.0	0.5662399571862123	8.094243659588489E-4
54-55	1.4283959399273804E-4	0.0	0.0	0.6484917567270306	9.046507619540076E-4
56-57	1.4283959399273804E-4	0.0	0.0	0.7364333334285598	9.522639599515869E-4
58-59	1.4283959399273804E-4	0.0	0.0	0.8370876339954425	9.760705589503765E-4
60-61	1.4283959399273804E-4	0.0	0.0	0.9387656183192732	0.0010236837569479558
62-63	1.4283959399273804E-4	0.0	0.0	1.0502281148316064	0.001237943147937063
64-65	1.4283959399273804E-4	0.0	0.0	1.1586671732710934	0.0012617497469358525
66-67	1.4283959399273804E-4	0.0	0.0	1.276652677909095	0.0012855563459346422
68-69	1.4283959399273804E-4	0.0	0.0	1.4118265470242228	0.0012855563459346422
70-71	1.4283959399273804E-4	0.0	0.0	1.5591179750297344	0.0012855563459346422
72-73	1.4283959399273804E-4	0.0	0.0	1.7073616669951979	0.0017854949249092255
74-75	1.4283959399273804E-4	0.0	0.0	1.8550816137826875	0.001833108122906805
76-77	1.4283959399273804E-4	0.0	0.0	2.0200851514432987	0.0018807213209043841
78-79	1.4283959399273804E-4	0.0	0.0	2.2046101002829177	0.0019045279199031739
80-81	1.4283959399273804E-4	0.0	0.0	2.3968007740001465	0.0019283345189019634
82-83	1.4283959399273804E-4	0.0	0.0	2.6038229588936215	0.002094980711893491
84-85	1.4283959399273804E-4	0.0	0.0	2.813820968661945	0.002094980711893491
86-87	1.4283959399273804E-4	0.0	0.0	3.0400788855464427	0.00219020710788865
88-89	1.4283959399273804E-4	0.0	0.0	3.2905005004147108	0.0022854335038838087
90-91	1.4283959399273804E-4	0.0	0.0	3.5638002569208163	0.0022854335038838087
92-93	1.4283959399273804E-4	0.0	0.0	3.8479796291693686	0.0022854335038838087
94-95	1.4283959399273804E-4	0.0	0.0	4.134420628322806	0.002475886295874126
96-97	1.4283959399273804E-4	0.0	0.0	4.440525878249243	0.002475886295874126
98-99	1.4283959399273804E-4	0.0	0.0	4.775246660172226	0.002475886295874126
100-101	1.4283959399273804E-4	0.0	0.0	5.1329646167280405	0.002475886295874126
102-103	1.4283959399273804E-4	0.0	0.0	5.511489540808796	0.0025949192908680746
104-105	1.4283959399273804E-4	0.0	0.0	5.902774801952903	0.002690145686863233
106-107	1.4283959399273804E-4	0.0	0.0	6.3257228397654	0.002737758884860812
108-109	1.4283959399273804E-4	0.0	0.0	6.766787699415977	0.0028567918798547607
110-111	1.666461929915277E-4	0.0	0.0	7.240491406293893	0.0032138908648366056
112-113	1.9045279199031738E-4	0.0	0.0	7.732740453791868	0.0033805370578281336
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2865	0.0	54.20926	1
GGTATCA	1690	0.0	53.16769	1
GTACATG	10120	0.0	41.512775	1
TCAACGC	3630	0.0	40.652	4
CAACGCA	3795	0.0	39.19717	5
TACATGG	10585	0.0	39.12508	2
ACATGGG	10550	0.0	38.63447	3
TATCAAC	4105	0.0	36.672813	2
AACGCAG	4110	0.0	36.479946	6
ATCAACG	4190	0.0	35.218796	3
CATGGGG	10005	0.0	34.61328	4
ATGGGGG	5975	0.0	31.269308	5
ACGCAGA	5205	0.0	28.688454	7
CGCAGAG	5775	0.0	25.650833	8
TGGGGGG	4185	0.0	23.741957	6
ATGGGGA	3645	0.0	23.669931	5
GCAGAGT	6735	0.0	21.993546	9
AGAGTAC	5820	0.0	21.51605	10-11
GTATATG	695	0.0	20.548683	1
GAGTACA	6880	0.0	20.15232	1
>>END_MODULE