FastQCFastQC Report
Mon 15 Aug 2016
SRR2921337_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921337_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2100258
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17800.064.11661
GTATCAA34000.053.601641
GTACATG100550.040.9443781
TACATGG106050.038.6582572
TCAACGC45650.038.3770034
ACATGGG110450.036.6326683
CAACGCA47700.036.4778255
TATCAAC50500.035.6352732
CATGGGG98900.035.4881634
ATCAACG49900.035.2278373
AACGCAG49900.034.509696
ATGGGGG61950.031.1650755
CGTATAC2006.033042E-826.814953
ACGCAGA63350.026.8040647
TACGGTC1401.0466958E-425.5343957
CGCAGAG67250.025.3345978
TGGGGGG39650.023.293376
AGAGTAC67650.022.40987810-11
GTAAGTA9450.022.0699182
ATGGGGA36400.021.7728235
Produced by FastQC (version 0.11.5)