##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921337_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2100258
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.08343784430294	31.0	30.0	34.0	16.0	34.0
2	29.871010609172778	33.0	31.0	34.0	26.0	34.0
3	30.125324126845367	33.0	31.0	34.0	26.0	34.0
4	32.26640631770002	37.0	35.0	37.0	19.0	37.0
5	31.914873315564087	37.0	33.0	37.0	19.0	37.0
6	32.08215371635294	37.0	35.0	37.0	17.0	37.0
7	32.28955490230248	37.0	35.0	37.0	17.0	37.0
8	32.54701327170281	37.0	35.0	37.0	17.0	37.0
9	34.26694149004551	39.0	37.0	39.0	17.0	39.0
10-11	34.46860171464649	39.0	37.0	39.0	20.0	39.0
12-13	34.493238211686375	39.0	37.0	39.0	22.5	39.0
14-15	35.907525408783115	40.5	38.0	41.0	25.0	41.0
16-17	35.934664931641734	41.0	38.0	41.0	25.0	41.0
18-19	35.94902364376186	41.0	38.0	41.0	25.0	41.0
20-21	35.935062739911004	41.0	38.0	41.0	25.0	41.0
22-23	35.87814877981658	41.0	38.0	41.0	24.0	41.0
24-25	35.825868536151276	41.0	38.0	41.0	24.0	41.0
26-27	35.74598501707885	40.0	38.0	41.0	23.0	41.0
28-29	35.678511401932525	40.0	37.5	41.0	22.5	41.0
30-31	35.59522092047739	40.0	37.0	41.0	21.5	41.0
32-33	35.48500255682873	40.0	37.0	41.0	19.0	41.0
34-35	35.40460410101997	40.0	37.0	41.0	18.5	41.0
36-37	35.33110194080918	40.0	37.0	41.0	18.0	41.0
38-39	35.241289641558325	40.0	36.0	41.0	16.0	41.0
40-41	35.12083634486811	40.0	36.0	41.0	15.0	41.0
42-43	34.98913347788701	40.0	36.0	41.0	14.0	41.0
44-45	34.86321204347276	40.0	35.0	41.0	9.0	41.0
46-47	34.684968941910945	40.0	35.0	41.0	8.0	41.0
48-49	34.505581457135264	40.0	35.0	41.0	4.5	41.0
50-51	34.33657626824895	40.0	35.0	41.0	2.0	41.0
52-53	34.15364707574022	39.0	35.0	41.0	2.0	41.0
54-55	33.94667797956251	39.0	35.0	41.0	2.0	41.0
56-57	33.701715217844665	39.0	35.0	41.0	2.0	41.0
58-59	33.63441967605885	38.5	35.0	41.0	2.0	41.0
60-61	33.389878291143276	38.0	34.5	41.0	2.0	41.0
62-63	33.10416696424915	37.0	34.0	40.0	2.0	41.0
64-65	32.80095088317721	37.0	34.0	40.0	2.0	41.0
66-67	32.48154869544599	36.0	34.0	39.0	2.0	41.0
68-69	32.14711121205109	35.5	34.0	39.0	2.0	41.0
70-71	31.81590523640429	35.0	33.5	38.5	2.0	40.5
72-73	31.475881534554325	35.0	33.0	37.0	2.0	39.5
74-75	31.147262145888746	35.0	33.0	37.0	2.0	39.0
76-77	30.85463143099562	35.0	33.0	36.0	2.0	39.0
78-79	30.59834934565182	35.0	33.0	36.0	2.0	37.0
80-81	30.37412641684974	35.0	33.0	35.5	2.0	37.0
82-83	30.18003740492835	35.0	32.5	35.0	2.0	36.5
84-85	30.012626305910988	35.0	32.0	35.0	2.0	36.0
86-87	29.877900953121	35.0	32.0	35.0	2.0	36.0
88-89	29.75766881973548	35.0	32.0	35.0	2.0	36.0
90-91	29.662728579060285	35.0	32.0	35.0	2.0	35.0
92-93	29.59809961442832	35.0	32.0	35.0	2.0	35.0
94-95	29.52952708667221	35.0	32.0	35.0	2.0	35.0
96-97	29.466005366959678	35.0	32.0	35.0	2.0	35.0
98-99	29.413465393299298	35.0	32.0	35.0	2.0	35.0
100-101	29.36058903239507	35.0	32.0	35.0	2.0	35.0
102-103	28.67677994798734	34.0	30.0	35.0	2.0	35.0
104-105	29.235916254098306	35.0	31.0	35.0	2.0	35.0
106-107	29.298980887110062	35.0	32.0	35.0	2.0	35.0
108-109	29.37628305665304	35.0	32.0	35.0	2.0	35.0
110-111	29.487466063693127	35.0	32.0	35.0	2.0	35.0
112-113	29.448437049162532	35.0	32.0	35.0	2.0	35.0
114-115	29.393724485277524	35.0	32.0	35.0	2.0	35.0
116-117	29.33494123102971	35.0	32.0	35.0	2.0	35.0
118-119	29.259803319401712	35.0	32.0	35.0	2.0	35.0
120-121	29.169469893698775	35.0	32.0	35.0	2.0	35.0
122-123	29.05502871551971	35.0	32.0	35.0	2.0	35.0
124-125	28.526840273909205	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	149210.0
3	8377.0
4	6426.0
5	6015.0
6	5994.0
7	6469.0
8	6689.0
9	6924.0
10	7058.0
11	6979.0
12	7160.0
13	7796.0
14	8774.0
15	7479.0
16	5667.0
17	5262.0
18	4819.0
19	4290.0
20	4359.0
21	4473.0
22	5286.0
23	6542.0
24	8628.0
25	10918.0
26	13349.0
27	15884.0
28	18957.0
29	22521.0
30	27309.0
31	33521.0
32	43796.0
33	58489.0
34	89478.0
35	152559.0
36	350982.0
37	731325.0
38	240305.0
39	189.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.58281432803364	17.64715155782803	11.97467881545928	27.795355298679052
2	15.404059882166857	19.63649227856768	37.95900313199617	27.0004447072693
3	18.59466789318265	23.657712528651242	28.292238382141623	29.45538119602449
4	13.827729367650182	16.25519910166381	31.33680844923667	38.58026308144934
5	14.536655955761113	37.79245327115062	32.035417055189384	15.635473717898881
6	34.156217072106486	34.151739354941974	16.646008829677168	15.046034743274372
7	30.137377081658546	31.23568889515413	19.55287441279227	19.074059610395054
8	27.67027622373127	35.55538307477883	17.967423037199186	18.80691766429072
9	27.772256884543285	16.43914607997798	17.676561578170215	38.11203545730852
10-11	27.0688387648485	25.415291408676836	25.538088535824006	21.977781290650658
12-13	26.82258244318231	22.87133137247616	26.151026972743324	24.15505921159821
14-15	24.43645018850065	23.877185564821087	23.4382156858824	28.248148560795865
16-17	24.57807564594445	26.790232438109985	24.019477607036848	24.612214308908715
18-19	24.808737783643725	26.06320271128595	25.306795641297402	23.821263863772927
20-21	25.577667124705634	25.073324324916275	25.085846595989636	24.26316195438846
22-23	25.398879566224718	24.629355060187844	24.967027860386676	25.00473751320076
24-25	25.009808318787503	25.066039505622644	24.92027169995305	25.0038804756368
26-27	24.653399725176623	25.123889541189705	25.140078028508878	25.082632705124798
28-29	24.745150357717957	25.32772164181734	25.025782546715693	24.901345453749016
30-31	25.155028572680116	25.293654398650073	24.849613714124647	24.701703314545163
32-33	24.824283492789935	25.398427240843745	24.95967162129605	24.81761764507027
34-35	25.013522148231317	25.411997002273058	25.103082573664757	24.471398275830875
36-37	25.139863769117888	25.255468613856014	25.073871876693243	24.530795740332852
38-39	24.901726359332997	25.551194186618975	25.025853966512685	24.521225487535343
40-41	24.890727710595556	25.553193940934875	24.95436274971932	24.60171559875025
42-43	24.742055499848114	25.53524376528979	24.898583888265154	24.82411684659694
44-45	24.63199759267671	25.48405957744239	25.163003783344713	24.720939046536188
46-47	24.62964073937583	25.4504208530571	24.846137950670823	25.07380045689625
48-49	24.629164607395854	25.65456243947172	24.75924386432524	24.957029088807182
50-51	24.50294201950427	25.755740485216577	24.93867420097912	24.802643294300033
52-53	24.729509422175752	25.526744809447223	24.92860400960263	24.8151417587744
54-55	24.378528733136594	25.320674888513693	25.234899712321056	25.065896666028646
56-57	24.444830111348224	25.48917799622713	24.902131071515974	25.163860820908667
58-59	24.584122522090144	25.531529935845974	24.971670147191443	24.91267739487244
60-61	24.891203842575532	25.29267832809112	25.127389111242525	24.688728718090825
62-63	24.64299624141415	25.432518290610012	25.11393838280821	24.81054708516763
64-65	24.521011228144353	25.151267130038306	25.13203139804729	25.195690243770052
66-67	24.488848512896986	25.449897107879128	24.9960719111652	25.065182468058687
68-69	24.360173845308527	25.395618062161883	25.084894332029684	25.159313760499902
70-71	24.354555487944815	25.2124500894652	25.16886020669841	25.26413421589157
72-73	24.36843473516111	25.493486990645913	24.879133896883147	25.258944377309835
74-75	24.264066605150415	25.51886482517862	25.04666093403763	25.170407635633335
76-77	24.27870766353467	25.1101293269684	25.042994717791817	25.568168291705113
78-79	24.212049186338056	25.237542244809923	25.175502247819075	25.37490632103294
80-81	24.37355315394585	25.4308042154821	24.86249308418299	25.333149546389063
82-83	24.386813429588177	25.434470431727913	24.965670884243746	25.213045254440168
84-85	24.33039178996104	25.474394098248883	24.820212564361142	25.375001547428933
86-87	24.036951650701962	25.592927154663858	24.99481016141826	25.37531103321592
88-89	24.03442815120809	25.339339262128746	25.011903299499394	25.614329287163766
90-91	24.181658893800993	25.208677041597326	25.077478687111803	25.532185377489874
92-93	24.251048742449424	25.31334907905165	25.094137445789283	25.341464732709646
94-95	24.212060714474468	25.228055423693384	24.96442102085602	25.59546284097613
96-97	23.98248021429789	25.15417182874071	25.105344401200995	25.7580035557604
98-99	23.866240211021744	25.278471806433263	24.98843594178333	25.866852040761668
100-101	23.9535809410082	25.240470456486776	25.08520381781667	25.72074478468836
102-103	23.730774980978527	25.117699825450018	25.191833574732247	25.95969161883921
104-105	23.765895372353153	25.255307980092915	25.048261939309825	25.930534708244107
106-107	23.812091523106822	25.09748214087184	25.10479077198428	25.985635564037057
108-109	23.752905900600048	25.031680714580745	25.20158885657796	26.013824528241248
110-111	23.582623815976543	25.393214344550707	25.098988027162427	25.92517381231032
112-113	23.58023639158117	25.432116458849542	25.0815619518494	25.906085197719886
114-115	23.58519493356518	25.444541469746042	25.064420779581898	25.905842817106883
116-117	23.401844257514227	25.238674081025632	25.216629075885766	26.142852585574378
118-119	23.448491599470444	25.111504343878327	25.177163053334727	26.2628410033165
120-121	23.367431549432176	25.085983545343154	25.341499954886466	26.205084950338208
122-123	23.285686771609566	25.334946813943194	25.126992557689746	26.252373856757487
124-125	23.080532525305237	25.234507015093865	25.015319553749848	26.669640905851043
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	48.0
1	41.5
2	56.0
3	65.0
4	50.0
5	74.5
6	146.0
7	340.5
8	510.0
9	555.5
10	510.5
11	436.5
12	458.0
13	491.0
14	536.0
15	572.0
16	606.5
17	711.0
18	769.0
19	813.5
20	902.0
21	954.0
22	1042.5
23	1185.5
24	1304.0
25	1489.5
26	1842.0
27	2350.0
28	2990.5
29	3957.5
30	5409.0
31	7288.5
32	9929.0
33	12874.5
34	15937.5
35	19714.5
36	24073.5
37	29094.5
38	34135.5
39	39194.5
40	44429.5
41	49315.0
42	54704.5
43	60991.0
44	67465.5
45	73807.5
46	79694.0
47	84717.5
48	91014.5
49	96162.0
50	98149.5
51	99940.5
52	99979.0
53	98784.0
54	97882.5
55	95113.0
56	89909.5
57	82843.5
58	75694.5
59	67938.0
60	58037.5
61	48338.0
62	39693.0
63	30426.0
64	22228.0
65	16669.5
66	12516.0
67	9588.0
68	7288.5
69	5424.0
70	4107.5
71	3091.5
72	2313.0
73	1776.0
74	1329.0
75	981.0
76	754.5
77	524.5
78	397.5
79	321.5
80	260.0
81	216.5
82	191.0
83	185.5
84	174.0
85	162.0
86	143.0
87	123.5
88	140.0
89	144.5
90	113.0
91	106.5
92	105.5
93	91.5
94	82.5
95	81.5
96	66.0
97	47.0
98	30.0
99	13.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33286386720107725
2	0.0
3	0.0
4	0.017902562447089834
5	0.1733596539091864
6	0.046375254849642285
7	0.023330467018813878
8	0.008808441629552178
9	0.007380045689624798
10-11	0.00219020710788865
12-13	0.05599312084515331
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	1.4283959399273804E-4
92-93	2.1425939098910704E-4
94-95	4.7613197997579344E-5
96-97	1.9045279199031738E-4
98-99	2.3806598998789672E-5
100-101	0.0
102-103	0.0
104-105	2.3806598998789672E-5
106-107	7.141979699636902E-5
108-109	2.6187258898668637E-4
110-111	1.9045279199031738E-4
112-113	6.665847719661108E-4
114-115	1.9045279199031738E-4
116-117	4.285187819782141E-4
118-119	1.666461929915277E-4
120-121	7.141979699636902E-5
122-123	0.0017378817269116463
124-125	4.7613197997579344E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2100258.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.84739673093313
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.81862557081698	50.60268995657987
2	9.553100888723728	11.816688413510523
3	3.239022729074037	6.00975371236655
4	1.5726287440909754	3.8905197538505467
5	0.9223040410586972	2.8521051966950037
6	0.5926345902162755	2.199174397054798
7	0.4217937279850657	1.8260790823318152
8	0.30754517443151336	1.5216694732599871
9	0.22427092181358604	1.2483515408955244
>10	1.2712482803389848	13.839716925066451
>50	0.06247300905065814	2.603913111331883
>100	0.013966962976779657	1.3436629551072805
>500	1.5414376908359494E-4	0.0821301620875859
>1k	2.3121565362539245E-4	0.16354531986216322
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.7613197997579344E-5
2	0.0	0.0	0.0	0.0	4.7613197997579344E-5
3	0.0	0.0	0.0	0.0	4.7613197997579344E-5
4	0.0	0.0	0.0	4.7613197997579344E-5	9.522639599515869E-5
5	0.0	0.0	0.0	4.7613197997579344E-5	9.522639599515869E-5
6	0.0	0.0	0.0	4.7613197997579344E-5	9.522639599515869E-5
7	0.0	0.0	0.0	4.7613197997579344E-5	9.522639599515869E-5
8	0.0	0.0	0.0	4.7613197997579344E-5	9.522639599515869E-5
9	0.0	0.0	0.0	9.522639599515869E-5	9.522639599515869E-5
10-11	0.0	0.0	0.0	9.522639599515869E-5	9.522639599515869E-5
12-13	0.0	0.0	0.0	9.522639599515869E-5	1.9045279199031738E-4
14-15	0.0	0.0	0.0	9.522639599515869E-5	3.8090558398063475E-4
16-17	0.0	0.0	0.0	9.522639599515869E-5	4.285187819782141E-4
18-19	0.0	0.0	0.0	9.522639599515869E-5	4.285187819782141E-4
20-21	0.0	0.0	0.0	9.522639599515869E-5	4.7613197997579347E-4
22-23	0.0	0.0	0.0	1.666461929915277E-4	5.475517769721625E-4
24-25	4.7613197997579344E-5	0.0	0.0	3.332923859830554E-4	5.713583759709522E-4
26-27	4.7613197997579344E-5	0.0	0.0	3.570989849818451E-4	5.713583759709522E-4
28-29	4.7613197997579344E-5	0.0	0.0	0.0013569761429310114	5.713583759709522E-4
30-31	4.7613197997579344E-5	0.0	0.0	0.006999140105644164	5.713583759709522E-4
32-33	4.7613197997579344E-5	0.0	0.0	0.02109264671292765	6.189715739685315E-4
34-35	4.7613197997579344E-5	0.0	0.0	0.03813817159606106	6.665847719661108E-4
36-37	4.7613197997579344E-5	0.0	0.0	0.06553956704366798	6.665847719661108E-4
38-39	4.7613197997579344E-5	0.0	0.0	0.11015313356739981	6.665847719661108E-4
40-41	4.7613197997579344E-5	0.0	0.0	0.16490831126461605	6.665847719661108E-4
42-43	4.7613197997579344E-5	0.0	0.0	0.21575920672603083	6.665847719661108E-4
44-45	4.7613197997579344E-5	0.0	0.0	0.26772901234038865	6.665847719661108E-4
46-47	4.7613197997579344E-5	0.0	0.0	0.3257695006994379	6.903913709649006E-4
48-49	4.7613197997579344E-5	0.0	0.0	0.38966641241218936	8.570375639564282E-4
50-51	4.7613197997579344E-5	0.0	0.0	0.4595863936716346	8.570375639564282E-4
52-53	4.7613197997579344E-5	0.0	0.0	0.531410902850983	9.760705589503765E-4
54-55	4.7613197997579344E-5	0.0	0.0	0.6096870003590036	0.001095103553944325
56-57	4.7613197997579344E-5	0.0	0.0	0.6923435120828012	0.0011189101529431146
58-59	4.7613197997579344E-5	0.0	0.0	0.7873080354889732	0.0011427167519419044
60-61	4.7613197997579344E-5	0.0	0.0	0.8829629502661102	0.0011427167519419044
62-63	4.7613197997579344E-5	0.0	0.0	0.9893308345927025	0.001237943147937063
64-65	4.7613197997579344E-5	0.0	0.0	1.0920325026734812	0.001237943147937063
66-67	4.7613197997579344E-5	0.0	0.0	1.2021142164438845	0.0012617497469358525
68-69	4.7613197997579344E-5	0.0	0.0	1.3288605495134407	0.0012855563459346422
70-71	4.7613197997579344E-5	0.0	0.0	1.4668674039094245	0.0012855563459346422
72-73	4.7613197997579344E-5	0.0	0.0	1.6053980034833817	0.001523622335922539
74-75	4.7613197997579344E-5	0.0	0.0	1.7444999614333097	0.001523622335922539
76-77	4.7613197997579344E-5	0.0	0.0	1.8996951803064195	0.0015474289349213287
78-79	4.7613197997579344E-5	0.0	0.0	2.073269093606595	0.0016188487319176977
80-81	4.7613197997579344E-5	0.0	0.0	2.253675500819423	0.0016664619299152769
82-83	4.7613197997579344E-5	0.0	0.0	2.4485325136245164	0.0018569147219055944
84-85	4.7613197997579344E-5	0.0	0.0	2.644984568562529	0.0018569147219055944
86-87	4.7613197997579344E-5	0.0	0.0	2.856887106250756	0.0020235609148971222
88-89	4.7613197997579344E-5	0.0	0.0	3.0906679084188706	0.0021187873108922807
90-91	4.7613197997579344E-5	0.0	0.0	3.347112592833833	0.0021425939098910704
92-93	4.7613197997579344E-5	0.0	0.0	3.6161747747181536	0.0021425939098910704
94-95	4.7613197997579344E-5	0.0	0.0	3.887903295690339	0.00219020710788865
96-97	4.7613197997579344E-5	0.0	0.0	4.177058247129638	0.0022378203058862293
98-99	4.7613197997579344E-5	0.0	0.0	4.49430498538751	0.0022854335038838087
100-101	4.7613197997579344E-5	0.0	0.0	4.8329300495462935	0.0022854335038838087
102-103	9.522639599515869E-5	0.0	0.0	5.191909755849044	0.0023568533008801774
104-105	1.1903299499394837E-4	0.0	0.0	5.562721341854191	0.002404466498877757
106-107	1.4283959399273804E-4	0.0	0.0	5.9645053131567645	0.0024282730978765466
108-109	1.4283959399273804E-4	0.0	0.0	6.382787257565499	0.0024282730978765466
110-111	1.4283959399273804E-4	0.0	0.0	6.832922431434614	0.002594919290868074
112-113	1.4283959399273804E-4	0.0	0.0	7.303055148462713	0.002618725889866864
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1780	0.0	64.1166	1
GTATCAA	3400	0.0	53.60164	1
GTACATG	10055	0.0	40.944378	1
TACATGG	10605	0.0	38.658257	2
TCAACGC	4565	0.0	38.377003	4
ACATGGG	11045	0.0	36.632668	3
CAACGCA	4770	0.0	36.477825	5
TATCAAC	5050	0.0	35.635273	2
CATGGGG	9890	0.0	35.488163	4
ATCAACG	4990	0.0	35.227837	3
AACGCAG	4990	0.0	34.50969	6
ATGGGGG	6195	0.0	31.165075	5
CGTATAC	200	6.033042E-8	26.81495	3
ACGCAGA	6335	0.0	26.804064	7
TACGGTC	140	1.0466958E-4	25.534395	7
CGCAGAG	6725	0.0	25.334597	8
TGGGGGG	3965	0.0	23.29337	6
AGAGTAC	6765	0.0	22.409878	10-11
GTAAGTA	945	0.0	22.069918	2
ATGGGGA	3640	0.0	21.772823	5
>>END_MODULE