FastQCFastQC Report
Mon 15 Aug 2016
SRR2921336_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921336_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2288408
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC25630.11199925887341769No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21650.065.6958161
GTATCAA40750.056.225171
TCAACGC53050.042.6243674
GTACATG132650.041.5882031
CAACGCA55550.040.7051855
AACGCAG58200.039.259026
TACATGG137450.039.179922
TATCAAC58400.039.1271362
ATCAACG58300.038.5818443
ACATGGG141100.037.8290253
CATGGGG131050.036.280184
ATGGGGG88500.032.7443165
ACGCAGA70400.032.3640177
CGCAGAG75400.030.0600688
TGGGGGG65950.029.9539786
GCAGAGT85650.025.7675679
AGAGTAC76700.024.43104410-11
ATATGTA14300.022.8888681
TATGTAA14150.022.708952
CAGAGTA81750.022.4852410-11
Produced by FastQC (version 0.11.5)