##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921336_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2288408
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.668120370143786	31.0	31.0	34.0	28.0	34.0
2	30.758830593145976	33.0	31.0	34.0	27.0	34.0
3	30.96650335080108	34.0	31.0	34.0	27.0	34.0
4	34.343458421749965	37.0	35.0	37.0	32.0	37.0
5	34.409828579519036	37.0	35.0	37.0	32.0	37.0
6	34.462131315744394	37.0	35.0	37.0	32.0	37.0
7	34.39989416222981	37.0	35.0	37.0	31.0	37.0
8	34.41921720252682	37.0	35.0	37.0	32.0	37.0
9	36.0359861528189	39.0	37.0	39.0	32.0	39.0
10-11	36.02570192902664	39.0	37.0	39.0	32.0	39.0
12-13	35.94709924978413	39.0	37.0	39.0	31.0	39.0
14-15	37.27146907369665	41.0	38.0	41.0	31.0	41.0
16-17	37.25406549006995	41.0	38.0	41.0	31.0	41.0
18-19	37.25404189287924	41.0	38.0	41.0	31.0	41.0
20-21	37.27630103547969	41.0	38.0	41.0	31.0	41.0
22-23	37.22120465406519	41.0	38.0	41.0	31.0	41.0
24-25	37.192565967257586	41.0	38.0	41.0	31.0	41.0
26-27	37.14230985034138	41.0	38.0	41.0	31.0	41.0
28-29	37.06644182331122	40.0	38.0	41.0	30.0	41.0
30-31	36.98377671289386	40.0	38.0	41.0	30.0	41.0
32-33	36.90192526857099	40.0	38.0	41.0	30.0	41.0
34-35	36.78896464266862	40.0	38.0	41.0	30.0	41.0
36-37	36.68833770027023	40.0	37.5	41.0	30.0	41.0
38-39	36.5951228976651	40.0	37.0	41.0	30.0	41.0
40-41	36.495485944814035	40.0	37.0	41.0	30.0	41.0
42-43	36.325725351423344	40.0	37.0	41.0	29.0	41.0
44-45	36.11446931666032	40.0	36.0	41.0	28.5	41.0
46-47	35.90821544934295	40.0	36.0	41.0	28.0	41.0
48-49	35.713083287595566	40.0	35.0	41.0	27.0	41.0
50-51	35.54000750740252	39.5	35.0	41.0	27.0	41.0
52-53	35.349367333097945	39.0	35.0	41.0	26.5	41.0
54-55	35.139066547573684	39.0	35.0	41.0	26.0	41.0
56-57	34.89464007292406	39.0	35.0	40.5	26.0	41.0
58-59	34.629374001489246	38.0	35.0	40.0	26.0	41.0
60-61	34.33522475013197	37.5	34.0	40.0	25.5	41.0
62-63	34.00752291549409	37.0	34.0	40.0	24.5	41.0
64-65	33.658499271108994	36.5	34.0	39.5	23.5	41.0
66-67	33.31831452258513	36.0	34.0	39.0	23.0	41.0
68-69	32.95777064229805	35.5	33.0	39.0	22.0	40.0
70-71	32.895587019447575	35.0	34.0	38.5	23.0	40.0
72-73	32.604305482239184	35.0	33.5	37.0	22.5	39.5
74-75	32.273714958171794	35.0	33.0	37.0	22.0	39.0
76-77	31.972297553583104	35.0	33.0	36.5	21.5	39.0
78-79	31.686204339435974	35.0	33.0	36.0	20.5	37.5
80-81	31.41180746615114	35.0	33.0	35.5	20.0	37.0
82-83	31.190588391580523	35.0	33.0	35.0	19.5	37.0
84-85	30.983323777927712	35.0	33.0	35.0	18.5	36.0
86-87	30.817737920860267	35.0	32.5	35.0	18.0	36.0
88-89	30.68067888243705	35.0	32.0	35.0	17.0	36.0
90-91	30.555268335017182	35.0	32.0	35.0	15.0	35.0
92-93	30.440975341809676	35.0	32.0	35.0	11.0	35.0
94-95	30.315126279929103	35.0	32.0	35.0	8.5	35.0
96-97	30.218623165099928	35.0	32.0	35.0	7.0	35.0
98-99	30.135664182261205	35.0	32.0	35.0	6.0	35.0
100-101	30.03863777787877	35.0	32.0	35.0	2.0	35.0
102-103	29.20953759119877	33.5	30.0	35.0	2.0	35.0
104-105	29.783383251588003	34.5	31.0	35.0	2.0	35.0
106-107	29.8489840972414	35.0	31.0	35.0	2.0	35.0
108-109	29.78053017643707	35.0	31.0	35.0	2.0	35.0
110-111	29.694277856046646	35.0	31.0	35.0	2.0	35.0
112-113	29.59689312395342	35.0	31.0	35.0	2.0	35.0
114-115	29.46190539449259	34.5	31.0	35.0	2.0	35.0
116-117	29.322552621735284	34.0	31.0	35.0	2.0	35.0
118-119	29.31832107736033	34.5	31.0	35.0	2.0	35.0
120-121	29.479757543235294	35.0	32.0	35.0	2.0	35.0
122-123	29.334370662923746	35.0	32.0	35.0	2.0	35.0
124-125	28.758555074095177	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	70979.0
3	10848.0
4	6768.0
5	5754.0
6	6116.0
7	6942.0
8	7123.0
9	7493.0
10	8286.0
11	8755.0
12	8773.0
13	8475.0
14	8785.0
15	8019.0
16	6765.0
17	6428.0
18	6129.0
19	5992.0
20	6015.0
21	6324.0
22	7273.0
23	9357.0
24	12805.0
25	16226.0
26	17903.0
27	19964.0
28	24251.0
29	29450.0
30	36447.0
31	45182.0
32	58325.0
33	79329.0
34	124893.0
35	205433.0
36	426636.0
37	753697.0
38	210405.0
39	63.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.09497294604068	17.379393536769783	12.408807615318047	25.116825901871486
2	16.07423888621567	19.395863953355736	39.766324159454435	24.76357300097416
3	18.87145052953884	25.07360836808161	28.68399120152195	27.370949900857607
4	13.488782511443006	15.467735161242246	35.490601504580916	35.552880822733826
5	14.552800582120765	38.24170685499643	32.65269198076204	14.552800582120765
6	33.278386909375115	34.20054264511298	17.876908645122597	14.644161800389302
7	29.623407409479324	31.93873858439506	20.57715235724375	17.860701648881864
8	27.50732699507105	35.04602480470816	19.531937656952408	17.914710543268384
9	28.938585377770572	15.31600589283896	18.26841260885735	37.476996120533116
10-11	26.75721586454461	25.267718779034553	26.7173158898044	21.25774946661644
12-13	26.654814799276476	22.71483366961696	26.951351842698802	23.678999688407764
14-15	24.357203055682227	23.763804967220747	24.150102599724725	27.728889377372294
16-17	24.597287852057665	26.6065768560637	24.63980855631939	24.15632673555924
18-19	24.712746024549194	26.415063831181957	25.72687792593726	23.145312218331597
20-21	25.759785305806375	25.32725481781497	25.2563718219552	23.65658805442346
22-23	25.675683943443833	24.746293494572765	25.21367633421522	24.364346227768177
24-25	25.129879891691008	24.932503535425045	25.161104381130183	24.776512191753763
26-27	24.551570854001028	25.246542893208414	25.40227029443557	24.79961595835499
28-29	24.731800016999784	25.843799962810245	25.055953977348334	24.368446042841637
30-31	25.59267222542696	25.27590592060412	25.027750100512176	24.103671753456744
32-33	25.213571748220453	25.298113296600334	24.961017802452908	24.52729715272631
34-35	25.137562837952704	25.53083675701755	25.264560727585156	24.06703967744459
36-37	25.073216735740843	25.581598612558427	25.209587890783336	24.135596760917394
38-39	25.189632063321838	25.682782496819616	24.921020326914007	24.20656511294454
40-41	25.07177993255964	25.31900007167068	25.05912830974319	24.55009168602649
42-43	24.76977899132782	25.45856284054617	24.901780235954682	24.869877932171335
44-45	24.61543504054435	25.417974989456937	25.269541808424396	24.697048161574315
46-47	24.640397694681926	25.198918357870586	25.168435716041625	24.99224823140586
48-49	24.53542450937804	25.45550729869269	25.314851993997074	24.69421619793219
50-51	24.60838907953489	25.451077177215563	25.402000854356288	24.538532888893258
52-53	24.722969385369392	25.382371514037803	25.198325383420332	24.696333717172468
54-55	24.245867380339106	25.071036570176965	25.674596133827563	25.008499915656362
56-57	24.51539667538881	25.168300930301157	25.167798360091552	25.148504034218476
58-59	24.677919244696593	25.468459247825624	25.28687912051414	24.56674238696364
60-61	25.186608378383518	25.27186886753225	25.328898564271924	24.212624189812313
62-63	24.87173738809251	25.264653621026152	25.40089167643385	24.46271731444748
64-65	24.584738659574413	25.008630793447413	25.43912166053844	24.967508886439735
66-67	24.7419605622133	25.234878270586236	25.22902242831291	24.794138738887554
68-69	24.464232882525202	25.18545742815177	25.43599703005907	24.914312659263956
70-71	24.41157518611593	25.021899526349678	25.570299937748313	24.996225349786076
72-73	24.41800707162213	25.663756647399094	25.11262722867315	24.80560905230562
74-75	24.29349545603127	25.513720181024585	25.357644163285713	24.835140199658436
76-77	24.29849016506384	24.896096288221393	25.374443390530903	25.430970156183868
78-79	24.219151323401043	25.048359555177306	25.566030212021662	25.166458909399985
80-81	24.64108057982972	25.306479362433777	25.11577235246536	24.93666770527114
82-83	24.531001450871397	25.376527348051813	25.400825073854598	24.691646127222196
84-85	24.460671233155093	25.479379676045472	25.084307847485665	24.97564124331377
86-87	24.031749153283076	25.74714301321971	25.28148148148148	24.939626352015733
88-89	24.071440292602965	25.16149078230302	25.510987797211758	25.256081127882258
90-91	24.554311319243737	24.878440596001063	25.525104068134887	25.04214401662031
92-93	24.665624225653225	25.056779967302077	25.408953426223626	24.868642380821065
94-95	24.575991385554765	25.03133945839385	25.330541971056913	25.06212718499447
96-97	24.05119855676289	25.029351808493132	25.536171720043292	25.383277914700685
98-99	23.84143005228445	25.03688404948406	25.41072836383405	25.710957534397433
100-101	23.958759201204256	25.23422687377675	25.484377700653283	25.322636224365713
102-103	23.842450362324225	25.095704987409768	25.632942093675513	25.428902556590486
104-105	23.99661930813809	25.12438332745558	25.418402133646577	25.460595230759754
106-107	24.0541343118461	24.905948232141416	25.62329166875161	25.416625787260877
108-109	24.030334952255032	24.827187920558558	25.68753915380368	25.454937973382734
110-111	23.743903310397844	25.517479780764994	25.566819237318633	25.171797671518526
112-113	23.69479420997177	25.56537030668026	25.54124737411828	25.198588109229686
114-115	23.900040444067557	25.650956116547963	25.32561188858938	25.123391550795105
116-117	23.616666470016796	25.39606376874674	25.568460157206278	25.418809604030184
118-119	23.587570806944193	25.095499915300866	25.822730621297694	25.494198656457247
120-121	23.65550490580946	25.144499639338164	25.98672860595733	25.21326684889504
122-123	23.551208815411588	25.376514842970238	25.74247625806474	25.329800083553433
124-125	23.40348314265494	25.270515252268595	25.599614921486502	25.726386683589965
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	522.0
1	434.5
2	323.0
3	263.0
4	219.0
5	202.0
6	200.5
7	212.5
8	255.0
9	287.5
10	297.5
11	344.0
12	395.5
13	383.5
14	414.5
15	486.5
16	515.5
17	571.5
18	623.0
19	674.5
20	723.5
21	769.0
22	848.5
23	1005.5
24	1197.0
25	1531.5
26	2066.0
27	2806.5
28	3893.5
29	5472.5
30	7691.5
31	10409.0
32	14030.0
33	17743.0
34	21708.0
35	26144.5
36	31090.0
37	36219.5
38	40553.5
39	45259.5
40	50365.0
41	55265.5
42	59832.5
43	65611.5
44	72335.5
45	79081.5
46	85079.0
47	90552.0
48	98356.5
49	104488.0
50	106795.5
51	107493.5
52	106434.0
53	105209.0
54	104109.0
55	101752.0
56	97043.0
57	89078.5
58	79809.5
59	70248.0
60	59235.0
61	49744.0
62	41424.0
63	31860.5
64	23593.5
65	17911.5
66	13638.0
67	10539.5
68	8198.0
69	6253.5
70	4804.0
71	3566.0
72	2652.0
73	2057.0
74	1633.0
75	1220.0
76	835.5
77	542.0
78	355.5
79	243.0
80	170.0
81	109.0
82	64.5
83	50.5
84	46.0
85	29.0
86	23.5
87	27.5
88	27.0
89	19.5
90	12.5
91	10.0
92	6.5
93	5.0
94	5.0
95	6.0
96	6.5
97	5.0
98	4.5
99	3.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01695501851068516
2	0.012279278869851881
3	0.012454072875116675
4	0.014376806933029425
5	0.009744765793512347
6	0.031681413454244176
7	0.013633932410654043
8	0.012847359386962463
9	0.009045589772453164
10-11	0.008127921244812987
12-13	0.007035458711908016
14-15	0.006401830442823133
16-17	0.004850533646098074
18-19	0.006358131941506934
20-21	0.005680805171105852
22-23	0.005855599176370647
24-25	0.00611779018426784
26-27	0.005003478400704769
28-29	0.006270734938874536
30-31	0.005593408168473454
32-33	0.00804052424218059
34-35	0.007909428738231994
36-37	0.0073194989704633085
38-39	0.007035458711908016
40-41	0.007166554215856612
42-43	0.007516142226386204
44-45	0.007559840727702403
46-47	0.009635519540221849
48-49	0.008302715250077784
50-51	0.005921146928344946
52-53	0.00537491566189246
54-55	0.006554775197429829
56-57	0.007450594474411905
58-59	0.007079157213224216
60-61	0.005527860416499155
62-63	0.005855599176370647
64-65	0.003932865118457898
66-67	0.004151357625038892
68-69	0.0064236796934812315
70-71	0.005090875403337167
72-73	0.004675739640833278
74-75	0.004369850131619886
76-77	0.004872382896756173
78-79	0.003517729355954008
80-81	0.003539578606612108
82-83	0.005593408168473454
84-85	0.009460725534957054
86-87	0.00690436320795942
88-89	0.005221970907285763
90-91	0.0053967649125505595
92-93	0.008259016748761583
94-95	0.006926212458617518
96-97	0.009548122537589451
98-99	0.006816966205327022
100-101	0.008237167498103486
102-103	0.005506011165841056
104-105	0.005200121656627664
106-107	0.007581689978360503
108-109	0.006838815455985122
110-111	0.007800182484941497
112-113	0.005637106669789653
114-115	0.0027311563322624287
116-117	0.0033647846013473122
118-119	0.1682829285686818
120-121	0.011077570083656412
122-123	0.002272322068442341
124-125	0.0019008848072546503
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2288408.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.16499959335772
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.037276415978	44.610008745861265
2	11.647310221777659	13.31636968183262
3	3.873576662052282	6.64299024933176
4	1.9040770955519088	4.353862655717865
5	1.0839772548509174	3.098277966638086
6	0.7121299867580961	2.4425346242066643
7	0.5044387707496961	2.0185369487346176
8	0.3483333531099027	1.592998079110442
9	0.2739993637866831	1.4096856167501413
>10	1.5204713204151779	15.314662718025168
>50	0.07274913606683577	2.7626224297743582
>100	0.02066691188295143	1.9196653193478272
>500	8.406597860803011E-4	0.30961373984239243
>1k	1.5284723383264705E-4	0.20817122482685835
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2563	0.11199925887341769	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	1.3109550394859658E-4
14-15	0.0	0.0	0.0	0.0	1.52944754606696E-4
16-17	0.0	0.0	0.0	0.0	1.7479400526479544E-4
18-19	0.0	0.0	0.0	2.184925065809943E-5	2.184925065809943E-4
20-21	0.0	0.0	0.0	4.369850131619886E-5	2.184925065809943E-4
22-23	0.0	0.0	0.0	8.739700263239772E-5	2.8404025855529257E-4
24-25	0.0	0.0	0.0	2.6219100789719315E-4	3.932865118457897E-4
26-27	0.0	0.0	0.0	5.462312664524857E-4	4.8068351447818746E-4
28-29	0.0	0.0	0.0	0.0019008848072546503	4.8068351447818746E-4
30-31	0.0	0.0	0.0	0.009701067292196146	4.8068351447818746E-4
32-33	0.0	0.0	0.0	0.02938724213514373	4.8068351447818746E-4
34-35	0.0	0.0	0.0	0.05294073434457492	4.8068351447818746E-4
36-37	0.0	0.0	0.0	0.08938528444228477	4.8068351447818746E-4
38-39	0.0	0.0	0.0	0.15189599057510722	4.8068351447818746E-4
40-41	0.0	0.0	0.0	0.22915494090214683	4.8068351447818746E-4
42-43	0.0	0.0	0.0	0.29673467318764835	4.8068351447818746E-4
44-45	0.0	0.0	0.0	0.37078178366794734	4.8068351447818746E-4
46-47	0.0	0.0	0.0	0.4481936787495936	5.025327651362869E-4
48-49	0.0	0.0	0.0	0.5359621186431791	6.11779018426784E-4
50-51	0.0	4.369850131619886E-5	0.0	0.6331694348210634	6.554775197429829E-4
52-53	0.0	4.369850131619886E-5	0.0	0.7342440683654313	7.428745223753806E-4
54-55	0.0	1.0924625329049716E-4	0.0	0.839863346046684	8.302715250077783E-4
56-57	0.0	1.7479400526479544E-4	0.0	0.9449145432108261	8.302715250077783E-4
58-59	0.0	1.7479400526479544E-4	0.0	1.067772879661319	8.739700263239772E-4
60-61	2.184925065809943E-5	1.7479400526479544E-4	0.0	1.1960716795256792	9.613670289563749E-4
62-63	4.369850131619886E-5	1.7479400526479544E-4	0.0	1.3356883912309343	0.0010924625329049714
64-65	4.369850131619886E-5	2.6219100789719315E-4	0.0	1.4699738857756133	0.0010924625329049714
66-67	4.369850131619886E-5	2.6219100789719315E-4	0.0	1.619291664773065	0.001114311783563071
68-69	4.369850131619886E-5	2.6219100789719315E-4	0.0	1.7919225942227086	0.0011361610342211703
70-71	4.369850131619886E-5	2.6219100789719315E-4	0.0	1.9774008830593144	0.0011798595355373691
72-73	4.369850131619886E-5	2.6219100789719315E-4	0.0	2.1533528986089894	0.001267256538169767
74-75	4.369850131619886E-5	2.6219100789719315E-4	0.0	2.3353353073402996	0.001267256538169767
76-77	4.369850131619886E-5	2.6219100789719315E-4	0.0	2.541788876808681	0.0013328042901440654
78-79	4.369850131619886E-5	2.6219100789719315E-4	0.0	2.7681034151252746	0.0013546535408021647
80-81	4.369850131619886E-5	2.6219100789719315E-4	0.0	2.998219723056378	0.001376502791460264
82-83	4.369850131619886E-5	2.6219100789719315E-4	0.0	3.2468860447962076	0.0013983520421183635
84-85	4.369850131619886E-5	2.6219100789719315E-4	0.0	3.5050349413216524	0.0013983520421183635
86-87	4.369850131619886E-5	2.8404025855529257E-4	0.0	3.783547339460446	0.001420201292776463
88-89	4.369850131619886E-5	3.05889509213392E-4	0.0	4.08917465766594	0.0014857490447507612
90-91	4.369850131619886E-5	3.05889509213392E-4	0.0	4.413876371695956	0.0014857490447507612
92-93	4.369850131619886E-5	3.05889509213392E-4	0.0	4.73779151270228	0.0014857490447507612
94-95	4.369850131619886E-5	3.05889509213392E-4	0.0	5.0780061946995465	0.0016168445486993577
96-97	4.369850131619886E-5	3.05889509213392E-4	0.0	5.443478610457576	0.001638693799357457
98-99	8.739700263239772E-5	3.05889509213392E-4	0.0	5.843188801996847	0.0016605430500155566
100-101	8.739700263239772E-5	3.05889509213392E-4	0.0	6.273356848953508	0.0016605430500155566
102-103	8.739700263239772E-5	3.05889509213392E-4	0.0	6.737063495670352	0.0016605430500155566
104-105	8.739700263239772E-5	3.05889509213392E-4	0.0	7.219385703947897	0.0016605430500155566
106-107	8.739700263239772E-5	3.05889509213392E-4	0.0	7.725742087949351	0.0016605430500155566
108-109	1.3109550394859658E-4	3.05889509213392E-4	0.0	8.25115101852467	0.0016823923006736561
110-111	1.3109550394859658E-4	3.277387598714914E-4	0.0	8.812261624675319	0.0017479400526479545
112-113	1.3109550394859658E-4	3.495880105295909E-4	0.0	9.38947512856099	0.0017479400526479545
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2165	0.0	65.695816	1
GTATCAA	4075	0.0	56.22517	1
TCAACGC	5305	0.0	42.624367	4
GTACATG	13265	0.0	41.588203	1
CAACGCA	5555	0.0	40.705185	5
AACGCAG	5820	0.0	39.25902	6
TACATGG	13745	0.0	39.17992	2
TATCAAC	5840	0.0	39.127136	2
ATCAACG	5830	0.0	38.581844	3
ACATGGG	14110	0.0	37.829025	3
CATGGGG	13105	0.0	36.28018	4
ATGGGGG	8850	0.0	32.744316	5
ACGCAGA	7040	0.0	32.364017	7
CGCAGAG	7540	0.0	30.060068	8
TGGGGGG	6595	0.0	29.953978	6
GCAGAGT	8565	0.0	25.767567	9
AGAGTAC	7670	0.0	24.431044	10-11
ATATGTA	1430	0.0	22.888868	1
TATGTAA	1415	0.0	22.70895	2
CAGAGTA	8175	0.0	22.48524	10-11
>>END_MODULE