FastQCFastQC Report
Mon 15 Aug 2016
SRR2921336_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921336_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2288408
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC25240.11029501732208592No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27850.072.971521
GTATCAA48200.052.0837141
GTACATG127450.043.6156961
TACATGG133550.041.6771972
ACATGGG136250.040.5451283
CATGGGG124550.038.1816444
ATGGGGG83700.037.450345
TCAACGC67850.035.3957144
TATCAAC71300.034.7695662
CAACGCA70200.034.2957045
ATCAACG71350.033.8264583
GTAAGTA13750.032.9386942
TGGGGGG61550.032.7167436
AACGCAG73350.032.651826
TGTAAGT15450.028.6287841
TAAGTAC17950.027.2235583
ACGCAGA89600.026.3894847
CGCAGAG93450.025.297858
AGAGTAC94850.023.66803210-11
CAGAGTA100000.022.9262569
Produced by FastQC (version 0.11.5)