FastQCFastQC Report
Mon 15 Aug 2016
SRR2921335_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921335_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334634
Sequences flagged as poor quality0
Sequence length125
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2950.062.5403481
ATTAGTC505.324808E-447.6042443
GTATCAA4800.047.115411
GTACATG16200.043.717221
CCTATAC853.4713375E-642.0037463
TACATGG17450.040.579552
TCCCGTA600.001306610439.6702042
ACATGGG17600.039.2194063
CAACGCA5650.038.968085
TCAACGC5950.038.0033874
ATGGGGG10750.034.8728755
CATGGGG17250.034.840794
AACGCAG6600.034.2555126
GTATAGA1051.47813535E-534.0081141
TATCAAC6650.034.0030332
CTACACG1102.0342337E-532.457444
ATCAACG7050.032.073783
AGGTTAG800.005354501329.7526535
GTGCTAT1254.86448E-528.5668181
GTACCGT850.00720529927.998316
Produced by FastQC (version 0.11.5)