##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921335_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 334634 Sequences flagged as poor quality 0 Sequence length 125 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.514024277270092 33.0 31.0 34.0 27.0 34.0 2 30.59975376082526 33.0 31.0 34.0 27.0 34.0 3 30.64507192933175 34.0 31.0 34.0 27.0 34.0 4 34.09653531918454 37.0 35.0 37.0 30.0 37.0 5 34.12423423800331 37.0 35.0 37.0 30.0 37.0 6 34.10004661809619 37.0 35.0 37.0 30.0 37.0 7 34.1189389004106 37.0 35.0 37.0 30.0 37.0 8 34.116736494199635 37.0 35.0 37.0 30.0 37.0 9 35.764865494839135 39.0 37.0 39.0 30.0 39.0 10-11 35.756855250811334 39.0 37.0 39.0 30.5 39.0 12-13 35.689179820341025 39.0 37.0 39.0 30.0 39.0 14-15 37.030470603704345 41.0 38.0 41.0 31.0 41.0 16-17 37.00295247942528 41.0 38.0 41.0 31.0 41.0 18-19 36.96738526270492 41.0 38.0 41.0 30.0 41.0 20-21 36.98751471757203 41.0 38.0 41.0 30.0 41.0 22-23 36.91515954744587 41.0 38.0 41.0 30.0 41.0 24-25 36.87340646796201 41.0 38.0 41.0 30.0 41.0 26-27 36.818093798000206 41.0 38.0 41.0 30.0 41.0 28-29 36.73508519755913 41.0 38.0 41.0 30.0 41.0 30-31 36.65811901958558 40.0 38.0 41.0 30.0 41.0 32-33 36.57448286784965 40.0 38.0 41.0 30.0 41.0 34-35 36.460162446135186 40.0 38.0 41.0 29.0 41.0 36-37 36.34404603238165 40.0 37.5 41.0 29.0 41.0 38-39 36.25695237184506 40.0 37.0 41.0 28.0 41.0 40-41 36.13969889491206 40.0 37.0 41.0 28.0 41.0 42-43 35.943964151879364 40.0 36.5 41.0 27.0 41.0 44-45 35.687114877747035 40.0 36.0 41.0 26.0 41.0 46-47 35.462777243197046 40.0 35.0 41.0 26.0 41.0 48-49 35.24605539186096 39.5 35.0 41.0 24.5 41.0 50-51 35.048230305348525 39.0 35.0 41.0 24.0 41.0 52-53 34.83287113682411 39.0 35.0 41.0 24.0 41.0 54-55 34.59697311092118 39.0 35.0 41.0 23.0 41.0 56-57 34.32966614271115 38.0 34.5 40.0 23.0 41.0 58-59 34.02405613296915 37.5 34.0 40.0 22.0 41.0 60-61 33.706032560947186 37.0 34.0 40.0 22.0 41.0 62-63 33.34825510856638 36.5 34.0 39.5 20.5 41.0 64-65 32.988678675807 36.0 33.0 39.0 20.0 41.0 66-67 32.62854192939152 35.0 33.0 39.0 19.0 40.5 68-69 32.26747282105225 35.0 33.0 37.5 18.0 40.0 70-71 32.187516809409686 35.0 33.0 37.0 19.0 39.5 72-73 31.900065444635032 35.0 33.0 37.0 18.0 39.0 74-75 31.5946526653 35.0 33.0 36.0 17.5 39.0 76-77 31.328984801305307 35.0 33.0 36.0 16.0 37.0 78-79 31.07794635332931 35.0 33.0 35.0 13.5 37.0 80-81 30.854511197308106 35.0 33.0 35.0 11.0 36.5 82-83 30.680597309299117 35.0 32.5 35.0 8.5 36.0 84-85 30.506923982619817 35.0 32.0 35.0 7.0 36.0 86-87 30.376792256614692 35.0 32.0 35.0 7.0 35.5 88-89 30.270171291620102 35.0 32.0 35.0 3.5 35.0 90-91 30.178004924783494 35.0 32.0 35.0 2.0 35.0 92-93 30.087072742160093 35.0 32.0 35.0 2.0 35.0 94-95 29.984324665156556 35.0 32.0 35.0 2.0 35.0 96-97 29.89732364314445 35.0 31.5 35.0 2.0 35.0 98-99 29.81716591858568 35.0 31.0 35.0 2.0 35.0 100-101 29.733168177770338 35.0 31.0 35.0 2.0 35.0 102-103 28.8825089500768 33.5 29.5 34.5 2.0 35.0 104-105 29.487174046869114 34.0 31.0 35.0 2.0 35.0 106-107 29.56921293114268 35.0 31.0 35.0 2.0 35.0 108-109 29.495783452966524 35.0 31.0 35.0 2.0 35.0 110-111 29.407724558771672 34.5 31.0 35.0 2.0 35.0 112-113 29.308330892856077 34.0 31.0 35.0 2.0 35.0 114-115 29.1726348787033 34.0 31.0 35.0 2.0 35.0 116-117 29.038456941016157 34.0 30.5 35.0 2.0 35.0 118-119 29.046402935744723 34.5 30.5 35.0 2.0 35.0 120-121 29.228963584094863 35.0 31.0 35.0 2.0 35.0 122-123 29.09607511490165 35.0 31.0 35.0 2.0 35.0 124-125 28.52200912041215 34.5 30.0 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13724.0 3 1693.0 4 1040.0 5 912.0 6 1055.0 7 1115.0 8 1133.0 9 1221.0 10 1192.0 11 1233.0 12 1288.0 13 1241.0 14 1160.0 15 1071.0 16 901.0 17 829.0 18 833.0 19 799.0 20 880.0 21 887.0 22 1029.0 23 1196.0 24 1613.0 25 2086.0 26 2533.0 27 2990.0 28 3490.0 29 4270.0 30 5126.0 31 6534.0 32 8411.0 33 11445.0 34 18581.0 35 31452.0 36 70171.0 37 108894.0 38 20603.0 39 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.89000077712619 15.554180639993303 10.799662846792561 26.756155736087944 2 17.558641384917028 18.731170197503705 36.94227918320501 26.767909234374255 3 20.215427193200057 23.116880011715796 27.13731092587667 29.530381869207478 4 14.409112510573177 14.21752502443413 32.776105663039154 38.597256801953534 5 15.447446047358182 36.644162116443866 32.18539206632416 15.722999769873788 6 35.810097785111914 32.33216445596381 16.746335674077528 15.111402084846745 7 32.04684095534986 30.069729421153152 18.880742664662158 19.002686958834833 8 29.66698945556273 33.11276092102432 18.199017287138656 19.0212323362743 9 29.378326763240555 14.352190844508469 16.714734441731398 39.554747950519584 10-11 27.946270333920104 23.830622143986417 25.803120731856747 22.419986790236727 12-13 27.967352842729404 21.03954478075838 25.846961220293597 25.146141156218622 14-15 25.442716232754204 22.169358104422905 22.940403842676783 29.44752182014611 16-17 25.99662003146867 25.053157551061883 23.491080140518232 25.459142276951212 18-19 25.9757240134425 24.669130227533206 24.723820681060516 24.63132507796378 20-21 26.725909145925076 23.765777531390693 24.286975869174434 25.221337453509797 22-23 26.6245186625206 23.3419352717829 24.214431831256565 25.819114234439933 24-25 26.076015946732657 23.76529374144537 24.140053912866765 26.018636398955213 26-27 25.814506006813698 23.751867790329328 24.464467156774848 25.969159046082126 28-29 25.825062535302184 24.297617233145356 24.192121597915193 25.685198633637267 30-31 26.529413786353512 23.999181144813345 24.03026177963531 25.441143289197836 32-33 26.0665688387064 23.8877050576657 24.270698533477187 25.775027570150716 34-35 26.100522261489377 24.123400153913973 24.437354771706726 25.33872281288992 36-37 26.159384231926158 24.198722977587106 24.23264341846318 25.409249372023556 38-39 26.149489696806683 24.33242181079183 23.991123862464697 25.526964629936792 40-41 26.17115703590801 23.986939824569266 24.119783027749136 25.722120111773584 42-43 25.796010830648587 24.03976617275243 24.109848598052636 26.054374398546347 44-45 25.681436796466283 24.114965235179938 24.297717760168318 25.90588020818546 46-47 25.7153744424271 23.97612056276571 24.18771807918469 26.120786915622503 48-49 25.726086514724955 24.13060890251702 24.133597527809158 26.009707054948866 50-51 25.739662654059032 24.00078299642571 24.429339055383547 25.83021529413171 52-53 25.878137378983872 24.073224561398266 24.215776662398074 25.832861397219787 54-55 25.5096978572069 23.729266907743344 24.4453244075192 26.31571082753056 56-57 25.71540004124291 24.024590335412412 24.145031574731846 26.114978048612837 58-59 25.877117611986687 24.147613077528842 24.179142838784294 25.79612647170017 60-61 26.089828072094363 23.99414853399558 24.264308149316978 25.65171524459307 62-63 26.095220448697447 24.098884382854223 24.144758272989993 25.661136895458338 64-65 25.859848247131843 23.825319837548527 24.26073521545142 26.05409669986821 66-67 25.77001639175242 24.15250754961979 24.121875742447767 25.95560031618002 68-69 25.666635286804468 23.97092730481352 24.169815859980066 26.192621548401945 70-71 25.551673848986006 23.975398812974795 24.370177336544955 26.10275000149424 72-73 25.650217112596714 24.29389843374106 23.977269670879416 26.078614782782804 74-75 25.439749926783055 24.195659580063474 24.31474917967139 26.04984131348208 76-77 25.52888509020707 23.695456623802 24.371150472627075 26.404507813363853 78-79 25.3750857306794 23.835743753800916 24.43014313101142 26.35902738450826 80-81 25.603812039781964 24.079713349909152 24.156366548723344 26.16010806158554 82-83 25.684366557486793 24.088806273459724 24.295311640806176 25.931515528247306 84-85 25.587873136558713 24.299304235555717 23.93827182632189 26.17455080156368 86-87 25.203971237814027 24.430075969946742 24.230288757523773 26.135664034715454 88-89 25.262052157905195 23.856421566649782 24.468910117043738 26.412616158401285 90-91 25.45877283030822 23.80837110056169 24.423704221711187 26.309151847418903 92-93 25.518675919450573 24.002695740013507 24.43589697610893 26.04273136442699 94-95 25.490427547623174 23.861646234676005 24.373591627167475 26.274334590533343 96-97 25.147790170832884 23.903453717318797 24.535260433477983 26.413495678370335 98-99 25.0462108589915 24.025612097756326 24.349872611940857 26.578304431311313 100-101 25.00833083034843 23.92778851197174 24.593806644042672 26.470074013637156 102-103 24.864022425839924 23.988685470422634 24.686952727620483 26.46033937611696 104-105 24.946805013478055 23.90621544567238 24.56248617826895 26.584493362580613 106-107 24.927937741795215 23.785849946130753 24.80540575272746 26.480806559346576 108-109 24.91015566045489 23.743113179212 24.842613637212665 26.504117523120442 110-111 24.77865527005925 24.1428261892843 24.73053847475736 26.34798006589909 112-113 24.75774506039003 24.14555169873572 24.72696355379965 26.369739687074606 114-115 24.79562974130782 24.162982199503624 24.65562247945838 26.385765579730176 116-117 24.59812983566581 23.907651297131398 24.87127304459048 26.622945822612316 118-119 24.624510010821297 23.694596985303694 24.922956264303046 26.75793673957197 120-121 24.42515380787502 23.75552729538733 25.21492754554461 26.60439135119303 122-123 24.430146536949515 24.024767986898787 24.954015757914444 26.591069718237254 124-125 24.354656514099236 23.93568380410486 25.01210299197915 26.697556689816754 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 19.0 1 17.5 2 13.0 3 9.5 4 8.5 5 9.5 6 12.0 7 13.5 8 22.0 9 25.5 10 27.5 11 38.5 12 42.5 13 44.0 14 56.5 15 54.5 16 50.0 17 59.5 18 65.0 19 81.0 20 93.0 21 86.5 22 92.5 23 107.5 24 121.5 25 133.5 26 140.0 27 160.0 28 194.0 29 227.5 30 271.5 31 370.5 32 490.5 33 654.5 34 877.0 35 1204.0 36 1674.5 37 2140.0 38 2746.0 39 3586.5 40 4361.0 41 5150.5 42 6205.5 43 7497.0 44 8835.0 45 10259.5 46 11505.5 47 12776.0 48 14392.5 49 15659.0 50 16376.0 51 17066.0 52 17611.0 53 17921.5 54 18278.5 55 18265.0 56 17415.5 57 16043.5 58 14737.0 59 13400.5 60 11643.5 61 9866.5 62 8183.5 63 6350.5 64 4679.0 65 3431.0 66 2612.5 67 2015.5 68 1533.5 69 1138.0 70 889.5 71 681.5 72 493.0 73 372.0 74 278.0 75 207.0 76 152.0 77 103.5 78 68.0 79 41.5 80 27.0 81 23.5 82 11.0 83 6.5 84 9.0 85 6.0 86 4.0 87 5.0 88 3.0 89 2.5 90 2.0 91 1.0 92 1.0 93 0.5 94 1.5 95 2.0 96 0.5 97 0.0 98 0.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02032070859506207 2 0.017332369095788235 3 0.012849859846877483 4 0.018228870945570384 5 0.010459188247458418 6 0.03735424374092292 7 0.016435867246006083 8 0.014941697496369168 9 0.011056856147313185 10-11 0.009861520347603651 12-13 0.0077696826981119675 14-15 0.007022597823293508 16-17 0.004631926223874442 18-19 0.007022597823293508 20-21 0.006126095973511358 22-23 0.005827262023583976 24-25 0.005976678998547667 26-27 0.004183675298983367 28-29 0.006873180848329817 30-31 0.006126095973511358 32-33 0.009861520347603651 34-35 0.009413269422712575 36-37 0.008217933623003042 38-39 0.008666184547894118 40-41 0.008666184547894118 42-43 0.008367350597966734 44-45 0.008516767572930425 46-47 0.010907439172349494 48-49 0.009562686397676268 50-51 0.006574346898402434 52-53 0.005827262023583976 54-55 0.007172014798257201 56-57 0.009263852447748884 58-59 0.008815601522857808 60-61 0.005080177148765517 62-63 0.006275512948475051 64-65 0.003287173449201217 66-67 0.004333092273947059 68-69 0.007321431773220892 70-71 0.004781343198838134 72-73 0.0044825092489107505 74-75 0.0035860073991286004 76-77 0.0044825092489107505 78-79 0.0031377564742375253 80-81 0.0029883394992738337 82-83 0.005379011098692901 84-85 0.011355690097240568 86-87 0.008367350597966734 88-89 0.004631926223874442 90-91 0.005528428073656592 92-93 0.009562686397676268 94-95 0.008367350597966734 96-97 0.011355690097240568 98-99 0.007919099673075658 100-101 0.010010937322567342 102-103 0.005976678998547667 104-105 0.004781343198838134 106-107 0.008217933623003042 108-109 0.008217933623003042 110-111 0.009413269422712575 112-113 0.005229594123729209 114-115 0.0025400885743827586 116-117 0.003287173449201217 118-119 0.1704847684335722 120-121 0.013298110771768557 122-123 0.0025400885743827586 124-125 0.0017930036995643002 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 334634.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.9271767511937 #Duplication Level Percentage of deduplicated Percentage of total 1 86.5571979529804 65.72043668064003 2 7.910272529146252 12.01209320941199 3 2.4272634484798155 5.528857826233166 4 1.1025114828850955 3.348423369249492 5 0.6346681626397173 2.409428088155058 6 0.3580556526364281 1.6311692894694054 7 0.233842547654637 1.2428503113406142 8 0.17195254321843395 1.0444696913410647 9 0.12417143963773151 0.8485188160341797 >10 0.47022789513820556 5.594468056656684 >50 0.007869076466697374 0.38806115545671466 >100 0.001967269116556532 0.23122350601156644 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.4941697496369168E-4 0.0 22-23 0.0 0.0 0.0 2.9883394992738337E-4 0.0 24-25 0.0 0.0 0.0 2.9883394992738337E-4 0.0 26-27 0.0 0.0 0.0 4.4825092489107505E-4 0.0 28-29 0.0 0.0 0.0 0.0016435867246006085 0.0 30-31 0.0 0.0 0.0 0.005976678998547667 0.0 32-33 0.0 0.0 0.0 0.017332369095788235 0.0 34-35 0.0 0.0 0.0 0.03212464961719371 0.0 36-37 0.0 0.0 0.0 0.050502937537727784 0.0 38-39 0.0 0.0 0.0 0.08307583807981257 0.0 40-41 0.0 0.0 0.0 0.12610792686935576 0.0 42-43 0.0 0.0 0.0 0.16211741783560546 0.0 44-45 0.0 0.0 0.0 0.2063448424248582 1.4941697496369168E-4 46-47 0.0 0.0 0.0 0.2617785401363878 2.9883394992738337E-4 48-49 0.0 0.0 0.0 0.31736165482288115 2.9883394992738337E-4 50-51 0.0 0.0 0.0 0.3838522086817239 2.9883394992738337E-4 52-53 0.0 0.0 0.0 0.4563194415391144 5.976678998547667E-4 54-55 0.0 0.0 0.0 0.5356598552448346 0.0010459188247458418 56-57 0.0 0.0 0.0 0.6164944387001918 0.0011953357997095335 58-59 0.0 0.0 0.0 0.7101788820024265 0.0011953357997095335 60-61 0.0 0.0 0.0 0.8131271777524101 0.0011953357997095335 62-63 0.0 0.0 0.0 0.9372926839472379 0.0014941697496369168 64-65 0.0 0.0 0.0 1.0581710166928646 0.0014941697496369168 66-67 0.0 0.0 0.0 1.1895085376859496 0.0014941697496369168 68-69 0.0 0.0 0.0 1.3383278447497862 0.0014941697496369168 70-71 0.0 0.0 0.0 1.518823550505926 0.0017930036995643002 72-73 0.0 0.0 0.0 1.6896071528894254 0.0020918376494916836 74-75 0.0 0.0 0.0 1.877872541343677 0.0020918376494916836 76-77 0.0 0.0 0.0 2.0866080553679542 0.0020918376494916836 78-79 0.0 0.0 0.0 2.324479879510151 0.0020918376494916836 80-81 0.0 0.0 0.0 2.5575703604535107 0.0022412546244553752 82-83 0.0 0.0 0.0 2.8148663913409875 0.002390671599419067 84-85 0.0 0.0 0.0 3.0832192783757777 0.002390671599419067 86-87 0.0 0.0 0.0 3.3841450659526524 0.002390671599419067 88-89 0.0 0.0 0.0 3.7170460861717576 0.002390671599419067 90-91 0.0 0.0 0.0 4.082071756008056 0.002390671599419067 92-93 0.0 0.0 0.0 4.4621885403156885 0.002390671599419067 94-95 0.0 0.0 0.0 4.862476616243418 0.002390671599419067 96-97 0.0 0.0 0.0 5.28711965909023 0.002390671599419067 98-99 0.0 0.0 0.0 5.748668694753073 0.002390671599419067 100-101 0.0 0.0 0.0 6.239503457508801 0.002390671599419067 102-103 0.0 0.0 0.0 6.781588242677074 0.002390671599419067 104-105 0.0 0.0 0.0 7.350717500313776 0.002390671599419067 106-107 0.0 0.0 0.0 7.93897213074583 0.002390671599419067 108-109 0.0 0.0 0.0 8.565626923743553 0.0025400885743827586 110-111 0.0 0.0 0.0 9.217084934585248 0.0026895055493464503 112-113 0.0 0.0 0.0 9.90604660614283 0.0026895055493464503 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 295 0.0 62.540348 1 ATTAGTC 50 5.324808E-4 47.604244 3 GTATCAA 480 0.0 47.11541 1 GTACATG 1620 0.0 43.71722 1 CCTATAC 85 3.4713375E-6 42.003746 3 TACATGG 1745 0.0 40.57955 2 TCCCGTA 60 0.0013066104 39.670204 2 ACATGGG 1760 0.0 39.219406 3 CAACGCA 565 0.0 38.96808 5 TCAACGC 595 0.0 38.003387 4 ATGGGGG 1075 0.0 34.872875 5 CATGGGG 1725 0.0 34.84079 4 AACGCAG 660 0.0 34.255512 6 GTATAGA 105 1.47813535E-5 34.008114 1 TATCAAC 665 0.0 34.003033 2 CTACACG 110 2.0342337E-5 32.45744 4 ATCAACG 705 0.0 32.07378 3 AGGTTAG 80 0.0053545013 29.752653 5 GTGCTAT 125 4.86448E-5 28.566818 1 GTACCGT 85 0.007205299 27.99831 6 >>END_MODULE