FastQCFastQC Report
Mon 15 Aug 2016
SRR2921335_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921335_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334634
Sequences flagged as poor quality0
Sequence length125
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2650.067.6431
CGATATA300.004163836659.7513161
GTATCAA4250.059.048361
TATTGCA505.299165E-447.6508642
CTATACT558.475034E-443.318974
TATAGGA650.001926805136.6545142
GTAAGTA1001.0522068E-535.7381482
ATGGGGG10400.035.509065
GTATAGG851.666088E-435.147831
ATTCTAG851.666088E-435.147831
GTACATG15750.034.9023551
TATCAAC6700.034.671342
TCAACGC6250.034.3086284
TACATGG16450.034.0363352
TGTACTA700.002771620434.0363355
ACATGGG16300.033.9841273
CATGGGG16300.033.618714
ATCAACG6700.032.8933223
TATACTG1301.7168313E-632.07275
CCACGCG750.00389472831.7529939
Produced by FastQC (version 0.11.5)