FastQCFastQC Report
Mon 15 Aug 2016
SRR2921334_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921334_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2688193
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39100.057.3805351
GGTATCA20400.053.385211
TCAACGC47700.046.2831844
CAACGCA49550.044.7936745
AACGCAG52650.042.041676
TATCAAC53850.041.771672
ATCAACG53350.041.2708173
GTACATG126700.040.3474921
TACATGG132900.038.1944122
ACATGGG133050.037.6593673
CATGGGG122800.035.3261954
ATGGGGG77450.033.4978265
ACGCAGA65750.033.295887
CGCAGAG70250.031.0783658
GTATACG2154.0308805E-927.6797121
GCAGAGT84400.025.656029
TGGGGGG50100.025.178716
AGAGTAC71250.024.0037610-11
ATGGGGA47650.021.3542695
CAGAGTA78550.020.71273610-11
Produced by FastQC (version 0.11.5)