FastQCFastQC Report
Mon 15 Aug 2016
SRR2921334_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921334_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2688193
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23400.060.528081
GTATCAA39850.053.3882981
GTACATG119050.042.1671561
TACATGG124150.039.8448872
ACATGGG130800.037.409053
TCAACGC56500.036.0760424
CATGGGG117100.035.270994
CAACGCA59150.034.258275
TATCAAC61450.034.0428962
AACGCAG61750.033.1932266
ATGGGGG70200.032.7711335
ATCAACG62800.032.362053
ACGCAGA76850.026.3551777
CGCAGAG82850.024.3691848
AGAGTAC81200.023.324110-11
TGGGGGG46500.023.1928226
CAGAGTA88450.021.6142929
ATGGGGA45250.020.942085
GCAGAGT94500.020.230529
GTAAGTA9300.019.2255862
Produced by FastQC (version 0.11.5)