FastQCFastQC Report
Mon 15 Aug 2016
SRR2921333_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921333_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2152020
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39450.054.3022231
GGTATCA21950.054.2197531
TCAACGC49450.042.2359964
CAACGCA50650.041.235345
TATCAAC52200.040.466882
GTACATG127550.038.7688261
ATCAACG54050.038.531353
AACGCAG55800.037.8534776
TACATGG131750.037.2602042
ACATGGG132400.036.807633
CATGGGG117750.034.7671784
ATGGGGG73500.032.6256875
ACGCAGA66750.031.638667
CGCAGAG68750.030.5451988
TGGGGGG52750.028.537096
GTATAGG6000.027.769551
GCAGAGT83650.025.0332789
AGAGTAC74650.022.35342410-11
ATGGGGC27250.021.1810345
CAGAGTA79650.020.76347410-11
Produced by FastQC (version 0.11.5)