FastQCFastQC Report
Mon 15 Aug 2016
SRR2921333_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921333_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2152020
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21780.10120723785094934No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30200.066.8822861
GTATCAA51450.050.2925721
GTACATG122600.037.873191
TACATGG126500.036.604752
TCAACGC69300.035.945954
TATCAAC72450.035.3701552
ACATGGG130300.035.217073
CAACGCA71300.034.5197375
ATCAACG72650.034.124373
CATGGGG115650.033.803774
ATGGGGG73350.033.554975
AACGCAG75150.032.585836
TGGGGGG50750.027.5897526
ACGCAGA88800.027.3040247
CGCAGAG92650.026.100278
GTGGTAT14350.025.4026641
AGAGTAC96350.022.90264910-11
GCAGAGT104200.022.0634759
GTACTAA5150.022.046911
CAGAGTA100950.021.44605310-11
Produced by FastQC (version 0.11.5)