##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921333_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2152020
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.092324885456456	31.0	30.0	34.0	16.0	34.0
2	29.878287841191067	33.0	31.0	34.0	26.0	34.0
3	30.136404401446082	33.0	31.0	34.0	26.0	34.0
4	32.27769444521891	37.0	35.0	37.0	19.0	37.0
5	31.893997267683385	37.0	33.0	37.0	19.0	37.0
6	32.05325136383491	37.0	35.0	37.0	17.0	37.0
7	32.27865168539326	37.0	35.0	37.0	17.0	37.0
8	32.54435739444801	37.0	35.0	37.0	17.0	37.0
9	34.2791344875977	39.0	37.0	39.0	17.0	39.0
10-11	34.486987109785225	39.0	37.0	39.0	20.5	39.0
12-13	34.49518010985028	39.0	37.0	39.0	22.5	39.0
14-15	35.903057127721866	40.5	38.0	41.0	25.0	41.0
16-17	35.91719291642271	41.0	38.0	41.0	25.0	41.0
18-19	35.944958689975	41.0	38.0	41.0	25.0	41.0
20-21	35.93420855754129	41.0	38.0	41.0	25.0	41.0
22-23	35.88387282646072	41.0	38.0	41.0	24.0	41.0
24-25	35.82655295954498	41.0	38.0	41.0	23.5	41.0
26-27	35.74090993578126	40.5	38.0	41.0	23.0	41.0
28-29	35.67037364894378	40.0	37.0	41.0	22.5	41.0
30-31	35.5776268343231	40.0	37.0	41.0	21.5	41.0
32-33	35.4580310591909	40.0	37.0	41.0	19.0	41.0
34-35	35.37543099041831	40.0	37.0	41.0	18.0	41.0
36-37	35.29924930995065	40.0	36.0	41.0	17.0	41.0
38-39	35.203915623460745	40.0	36.0	41.0	16.0	41.0
40-41	35.07942653878681	40.0	36.0	41.0	15.0	41.0
42-43	34.939567011459005	40.0	35.0	41.0	11.0	41.0
44-45	34.79858528266466	40.0	35.0	41.0	8.5	41.0
46-47	34.594431975539266	40.0	35.0	41.0	4.5	41.0
48-49	34.39327771117369	40.0	35.0	41.0	2.0	41.0
50-51	34.21777144264458	40.0	35.0	41.0	2.0	41.0
52-53	34.0367659222498	39.0	35.0	41.0	2.0	41.0
54-55	33.838865577457454	39.0	35.0	41.0	2.0	41.0
56-57	33.59143386213883	39.0	34.5	41.0	2.0	41.0
58-59	33.52506017602067	38.5	35.0	41.0	2.0	41.0
60-61	33.28686629306419	38.0	34.0	41.0	2.0	41.0
62-63	32.99911246178009	37.0	34.0	40.0	2.0	41.0
64-65	32.69861037536826	37.0	34.0	40.0	2.0	41.0
66-67	32.380307571490974	36.0	34.0	39.0	2.0	41.0
68-69	32.04017016570478	35.5	34.0	39.0	2.0	41.0
70-71	31.709797771396175	35.0	33.0	38.5	2.0	40.5
72-73	31.368694761201105	35.0	33.0	37.0	2.0	39.5
74-75	31.038381845893625	35.0	33.0	37.0	2.0	39.0
76-77	30.74096941478239	35.0	33.0	36.0	2.0	39.0
78-79	30.488250806219273	35.0	33.0	36.0	2.0	37.5
80-81	30.26318342766331	35.0	33.0	35.5	2.0	37.0
82-83	30.062547048819248	35.0	32.0	35.0	2.0	37.0
84-85	29.89378909117945	35.0	32.0	35.0	2.0	36.0
86-87	29.755104041783998	35.0	32.0	35.0	2.0	36.0
88-89	29.638036124199587	35.0	32.0	35.0	2.0	36.0
90-91	29.537215035176253	35.0	32.0	35.0	2.0	35.0
92-93	29.469935688330033	35.0	32.0	35.0	2.0	35.0
94-95	29.40667256809881	35.0	32.0	35.0	2.0	35.0
96-97	29.341185490841163	35.0	32.0	35.0	2.0	35.0
98-99	29.285774760457617	35.0	32.0	35.0	2.0	35.0
100-101	29.234525701434002	35.0	31.5	35.0	2.0	35.0
102-103	28.564167386920197	34.0	30.0	35.0	2.0	35.0
104-105	29.106743664092342	35.0	31.0	35.0	2.0	35.0
106-107	29.16580770624808	35.0	31.0	35.0	2.0	35.0
108-109	29.241438044256093	35.0	31.5	35.0	2.0	35.0
110-111	29.35285197163595	35.0	32.0	35.0	2.0	35.0
112-113	29.319977277162852	35.0	32.0	35.0	2.0	35.0
114-115	29.26329216271224	35.0	32.0	35.0	2.0	35.0
116-117	29.201335257107274	35.0	32.0	35.0	2.0	35.0
118-119	29.128218603916324	35.0	32.0	35.0	2.0	35.0
120-121	29.040167377626602	35.0	32.0	35.0	2.0	35.0
122-123	28.933291512160668	35.0	32.0	35.0	2.0	35.0
124-125	28.41761833068466	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	152488.0
3	8831.0
4	6743.0
5	6150.0
6	6498.0
7	6799.0
8	7091.0
9	7066.0
10	7456.0
11	7531.0
12	8095.0
13	9587.0
14	11266.0
15	9844.0
16	6536.0
17	5693.0
18	5219.0
19	4744.0
20	4675.0
21	4667.0
22	5357.0
23	6706.0
24	8971.0
25	11486.0
26	13606.0
27	15995.0
28	18718.0
29	22513.0
30	27406.0
31	33963.0
32	43258.0
33	58914.0
34	90732.0
35	154889.0
36	347066.0
37	747309.0
38	257895.0
39	257.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.761702413418746	18.04495920430677	12.398803550176652	26.79453483209783
2	15.518071393388539	19.648655681638648	38.526082471352495	26.307190453620322
3	18.46423360377692	24.31803607773162	28.62333993178502	28.594390386706444
4	13.642887219666541	16.498437931358215	31.918346707664963	37.94032814131028
5	14.22895642959964	37.61650524308511	31.83076874372664	16.323769583588614
6	33.05934434186388	34.671943124348545	16.992911929586068	15.275800604201518
7	29.52614635787877	31.68128150758599	19.719484295824337	19.073087838710904
8	27.732348745021678	35.57855406793288	17.942448985282294	18.74664820176315
9	27.906910819974424	16.19417883591589	17.88791340748892	38.010996936620764
10-11	26.829059181669606	25.55664190017784	25.623185671527494	21.991113246625062
12-13	26.521364143574484	22.99153803236005	26.193044448577275	24.29405337548819
14-15	24.307464614641127	24.044037694816964	23.86206912575162	27.786428564790288
16-17	24.179770634101914	26.949075752084088	24.351446547894536	24.519707065919462
18-19	24.32168381334746	26.216740550738376	25.86820754453955	23.593368091374614
20-21	25.42736591667364	25.07867027258111	25.56532931850076	23.9286344922445
22-23	25.224649399169152	24.568591369968683	25.406501798310426	24.800257432551742
24-25	24.682368193604148	25.051021830652132	25.356688134868634	24.909921840875086
26-27	24.29213018466371	25.111964572819957	25.701968383193464	24.893936859322867
28-29	24.421450544139926	25.4916311186699	25.53610096560441	24.550817371585765
30-31	25.044051635207854	25.335080528991366	25.37931803607773	24.24154979972305
32-33	24.66478006709975	25.395349485599578	25.418769342292357	24.521101105008317
34-35	24.73666601611509	25.53459075659148	25.603711861413924	24.125031365879497
36-37	24.877440730104738	25.350926106634695	25.584869099729556	24.186764063531008
38-39	24.679278073623852	25.580686982462986	25.485799388481517	24.25423555543164
40-41	24.691824425423555	25.542327673534633	25.412984080073603	24.352863820968206
42-43	24.48436817501696	25.585194375516956	25.272836683673944	24.65760076579214
44-45	24.29717195936841	25.552782966701056	25.64699677512291	24.50304829880763
46-47	24.26385442514475	25.51493480543861	25.26779490896925	24.953415860447397
48-49	24.37291474986292	25.77206531537811	25.109710876292972	24.745309058466
50-51	24.373263259635134	25.77706062211318	25.25994182210203	24.589734296149665
52-53	24.521426380795717	25.639422496073454	25.184524307394913	24.654626815735913
54-55	24.069804183975986	25.36777074562504	25.63161587717586	24.930809193223112
56-57	24.277748348063678	25.55566398081802	25.174185184152563	24.992402486965734
58-59	24.384694380163754	25.60092378323622	25.35008968318138	24.66429215341865
60-61	24.754068270740976	25.418444066504957	25.48591555840559	24.341572104348472
62-63	24.55351251382422	25.456687205509244	25.47223074134999	24.51756953931655
64-65	24.331674426817596	25.146931719965426	25.48238399271382	25.039009860503153
66-67	24.340921552773672	25.468977983475988	25.338193882956478	24.85190658079386
68-69	24.080631220899434	25.409243408518506	25.46809509205305	25.04203027852901
70-71	24.207442310015708	25.293259356325688	25.511147665913885	24.988150667744723
72-73	24.21634092619957	25.726201429354745	25.10480850549716	24.952649138948523
74-75	24.06694640384383	25.695718441278427	25.287125584334717	24.950209570543024
76-77	24.09259672307878	25.176810624436573	25.377947230973692	25.35264542151095
78-79	23.93827659594242	25.341562810754546	25.51916339067481	25.20099720262823
80-81	24.32342636220853	25.504874490014036	25.10506407933012	25.06663506844732
82-83	24.248427059228074	25.5274579232535	25.280225090844883	24.943889926673542
84-85	24.23838997778831	25.659287553089655	25.01442830456966	25.087894164552377
86-87	23.779077331995055	25.97027443982863	25.13773106197898	25.11291716619734
88-89	23.792994488898803	25.529014600236057	25.31686508489698	25.361125825968163
90-91	24.18551277675535	25.281579320947568	25.331834581892725	25.201073320404355
92-93	24.127321869479726	25.444253176274	25.281475160088668	25.146949794157603
94-95	24.129915300409966	25.43243723622136	25.118963948945584	25.318683514423096
96-97	23.822403241620624	25.401066909540383	25.23250356643324	25.544026282405753
98-99	23.5822677257339	25.441567792485152	25.17017155281353	25.80599292896742
100-101	23.713232219031422	25.561077499279744	25.280689770541166	25.445000511147665
102-103	23.61065417607643	25.360149998605962	25.327552717911544	25.701643107406063
104-105	23.74178678636816	25.476110816813968	25.194607856804307	25.587494540013566
106-107	23.795669687323347	25.1951252209847	25.335830527242948	25.673374564449002
108-109	23.69975597226685	25.204674979362313	25.354441976208747	25.74112707216209
110-111	23.513628993153617	25.72646568106165	25.22937261255342	25.53053271323132
112-113	23.50451567574123	25.733159820604378	25.251631793349777	25.510692710304607
114-115	23.59316602641341	25.726354812912376	25.12647460772774	25.554004552946473
116-117	23.365131542261924	25.559988624577606	25.307759078962533	25.767120754197943
118-119	23.381248161604972	25.35235614282999	25.36148712856289	25.90490856700215
120-121	23.352430137666136	25.41574931064703	25.517793066786616	25.71402748490022
122-123	23.29476121127182	25.629883279909816	25.250233969923595	25.82512153889477
124-125	23.077739841799612	25.54196236787108	25.127328193195453	26.252969597133852
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	39.0
1	48.5
2	76.5
3	83.0
4	75.0
5	103.5
6	184.5
7	499.0
8	733.0
9	799.5
10	789.5
11	674.0
12	690.0
13	758.5
14	827.0
15	926.0
16	1018.5
17	1098.0
18	1204.0
19	1273.5
20	1351.5
21	1408.0
22	1527.5
23	1768.5
24	1960.5
25	2208.5
26	2784.5
27	3563.0
28	4459.0
29	5715.5
30	7567.5
31	9676.5
32	12490.0
33	15944.5
34	19504.5
35	23365.5
36	27923.0
37	33068.0
38	37716.0
39	42277.0
40	46839.5
41	50777.0
42	55028.5
43	60828.5
44	67323.5
45	73578.5
46	78565.5
47	83954.0
48	90752.5
49	96348.5
50	99852.5
51	102126.0
52	102306.0
53	100839.0
54	99601.5
55	96620.5
56	91232.5
57	83546.5
58	75199.0
59	67060.0
60	57111.0
61	47191.5
62	38731.0
63	29848.5
64	21797.0
65	16230.5
66	12199.5
67	9178.0
68	6889.5
69	5170.0
70	3937.0
71	3011.0
72	2197.5
73	1632.0
74	1251.5
75	891.5
76	624.0
77	458.0
78	365.0
79	285.0
80	246.0
81	237.0
82	219.5
83	180.5
84	163.5
85	157.5
86	143.0
87	138.0
88	128.5
89	122.0
90	122.5
91	111.5
92	102.0
93	98.5
94	82.5
95	69.5
96	59.5
97	49.5
98	34.0
99	11.0
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35668813486863504
2	0.0
3	0.0
4	0.017936636276614532
5	0.18810234105630988
6	0.05027834313807493
7	0.025743255174208418
8	0.008828914229421659
9	0.0068772595050231875
10-11	0.0024628023903123576
12-13	0.056690922946812765
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	3.020418025854778E-4
92-93	4.41445711471083E-4
94-95	1.1616992407133763E-4
96-97	4.646796962853505E-4
98-99	2.3233984814267525E-5
100-101	0.0
102-103	0.0
104-105	0.0
106-107	2.0910586332840774E-4
108-109	4.8791368109961803E-4
110-111	3.949777418425479E-4
112-113	7.667214988708283E-4
114-115	3.252757873997454E-4
116-117	5.576156355424206E-4
118-119	3.252757873997454E-4
120-121	9.29359392570701E-5
122-123	0.0015799109673701917
124-125	1.1616992407133763E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2152020.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.15454503786408
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.38600596581726	51.20664718292128
2	9.34893693668187	11.621578437742889
3	3.1029229478618596	5.785822923357058
4	1.4666898111098732	3.646457516848199
5	0.8693261915263545	2.7016286961909834
6	0.5912939534354812	2.2050963995653396
7	0.403259106587041	1.7545070411605153
8	0.2938799356080032	1.4612778954777785
9	0.21390662574348385	1.1965742103303816
>10	1.2460028001753807	13.789640662757229
>50	0.0601376572991611	2.5222765391007043
>100	0.01711555127292681	1.6673183773574338
>500	1.492905375334803E-4	0.05307636871023061
>1k	3.73226343833621E-4	0.388097748479887
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2178	0.10120723785094934	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.646796962853505E-5	0.0
5	0.0	4.646796962853505E-5	0.0	4.646796962853505E-5	0.0
6	0.0	4.646796962853505E-5	0.0	4.646796962853505E-5	9.29359392570701E-5
7	0.0	4.646796962853505E-5	0.0	4.646796962853505E-5	9.29359392570701E-5
8	0.0	4.646796962853505E-5	0.0	4.646796962853505E-5	9.29359392570701E-5
9	0.0	4.646796962853505E-5	0.0	4.646796962853505E-5	9.29359392570701E-5
10-11	0.0	4.646796962853505E-5	0.0	4.646796962853505E-5	9.29359392570701E-5
12-13	0.0	6.970195444280257E-5	0.0	4.646796962853505E-5	1.3940390888560514E-4
14-15	0.0	9.29359392570701E-5	0.0	4.646796962853505E-5	2.3233984814267526E-4
16-17	0.0	9.29359392570701E-5	0.0	4.646796962853505E-5	3.252757873997454E-4
18-19	0.0	1.1616992407133763E-4	0.0	4.646796962853505E-5	3.252757873997454E-4
20-21	0.0	1.3940390888560514E-4	0.0	4.646796962853505E-5	4.1821172665681543E-4
22-23	0.0	1.3940390888560514E-4	0.0	1.3940390888560514E-4	4.646796962853505E-4
24-25	0.0	1.3940390888560514E-4	0.0	3.0204180258547786E-4	5.111476659138855E-4
26-27	0.0	1.3940390888560514E-4	0.0	4.4144571147108295E-4	5.576156355424206E-4
28-29	0.0	1.3940390888560514E-4	0.0	0.0011616992407133763	6.040836051709556E-4
30-31	0.0	1.3940390888560514E-4	0.0	0.005088242674324588	6.273175899852232E-4
32-33	0.0	1.3940390888560514E-4	0.0	0.016496129218129943	7.667214988708283E-4
34-35	0.0	1.3940390888560514E-4	0.0	0.03129617754481835	8.364234533136309E-4
36-37	0.0	1.3940390888560514E-4	0.0	0.053740206875400785	8.364234533136309E-4
38-39	0.0	1.6263789369987266E-4	0.0	0.09149543219858551	8.364234533136309E-4
40-41	0.0	2.0910586332840774E-4	0.0	0.13924127099190528	8.364234533136309E-4
42-43	0.0	2.3233984814267526E-4	0.0	0.18426873356195575	8.364234533136309E-4
44-45	0.0	2.3233984814267526E-4	0.0	0.23020232153976264	8.828914229421659E-4
46-47	0.0	2.3233984814267526E-4	0.0	0.2808756424196801	9.29359392570701E-4
48-49	0.0	2.788078177712103E-4	0.0	0.33566137861172296	9.758273621992361E-4
50-51	0.0	2.788078177712103E-4	0.0	0.40243585096792783	9.758273621992361E-4
52-53	0.0	2.788078177712103E-4	0.0	0.4732530366818152	0.0011152312710848412
54-55	0.0	2.788078177712103E-4	0.0	0.5467188966645291	0.0011616992407133763
56-57	0.0	2.788078177712103E-4	0.0	0.6234607485060548	0.0011849332255276439
58-59	0.0	2.788078177712103E-4	0.0	0.7137480134942984	0.0012081672103419112
60-61	0.0	2.788078177712103E-4	0.0	0.805429317571398	0.0012081672103419112
62-63	0.0	2.788078177712103E-4	0.0	0.9169756786646964	0.0012546351799704464
64-65	0.0	2.788078177712103E-4	0.0	1.0159059860038475	0.0012546351799704464
66-67	0.0	2.788078177712103E-4	0.0	1.1223873384076357	0.0012546351799704464
68-69	0.0	2.788078177712103E-4	0.0	1.2409271289300285	0.0012546351799704464
70-71	0.0	2.788078177712103E-4	0.0	1.3719668032824974	0.0013011031495989815
72-73	0.0	2.788078177712103E-4	0.0	1.4987546584139553	0.0013940390888560516
74-75	0.0	2.788078177712103E-4	0.0	1.6330470906404215	0.0014405070584845865
76-77	0.0	2.788078177712103E-4	0.0	1.783068930586147	0.0014405070584845865
78-79	0.0	2.788078177712103E-4	0.0	1.9505627271122017	0.0014405070584845865
80-81	0.0	2.788078177712103E-4	0.0	2.119334392803041	0.0014869750281131216
82-83	0.0	2.788078177712103E-4	0.0	2.2992583712047283	0.0016496129218129944
84-85	0.0	2.788078177712103E-4	0.0	2.4929368686164626	0.0017193148762557969
86-87	0.0	2.788078177712103E-4	0.0	2.700811330749714	0.0017425488610700644
88-89	0.0	2.788078177712103E-4	0.0	2.9401910762911125	0.001765782845884332
90-91	0.0	2.788078177712103E-4	2.3233984814267525E-5	3.194161764295871	0.001765782845884332
92-93	0.0	2.788078177712103E-4	4.646796962853505E-5	3.44650607336363	0.001905186754769937
94-95	0.0	2.788078177712103E-4	4.646796962853505E-5	3.7069125751619407	0.002044590663655542
96-97	0.0	2.788078177712103E-4	4.646796962853505E-5	3.9866265183409078	0.002114292618098345
98-99	0.0	2.788078177712103E-4	4.646796962853505E-5	4.302585477830132	0.0021375266029126125
100-101	0.0	2.788078177712103E-4	4.646796962853505E-5	4.635110268491928	0.0021375266029126125
102-103	0.0	2.788078177712103E-4	4.646796962853505E-5	4.99221661508722	0.00225369652698395
104-105	0.0	2.788078177712103E-4	4.646796962853505E-5	5.363216884601444	0.0022769305117982174
106-107	0.0	2.788078177712103E-4	4.646796962853505E-5	5.760727130788748	0.002300164496612485
108-109	0.0	2.788078177712103E-4	4.646796962853505E-5	6.169436157656527	0.00234663246624102
110-111	0.0	2.788078177712103E-4	4.646796962853505E-5	6.614064925047165	0.002486036375126625
112-113	4.646796962853505E-5	2.788078177712103E-4	4.646796962853505E-5	7.064734528489512	0.002602206299197963
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3020	0.0	66.882286	1
GTATCAA	5145	0.0	50.292572	1
GTACATG	12260	0.0	37.87319	1
TACATGG	12650	0.0	36.60475	2
TCAACGC	6930	0.0	35.94595	4
TATCAAC	7245	0.0	35.370155	2
ACATGGG	13030	0.0	35.21707	3
CAACGCA	7130	0.0	34.519737	5
ATCAACG	7265	0.0	34.12437	3
CATGGGG	11565	0.0	33.80377	4
ATGGGGG	7335	0.0	33.55497	5
AACGCAG	7515	0.0	32.58583	6
TGGGGGG	5075	0.0	27.589752	6
ACGCAGA	8880	0.0	27.304024	7
CGCAGAG	9265	0.0	26.10027	8
GTGGTAT	1435	0.0	25.402664	1
AGAGTAC	9635	0.0	22.902649	10-11
GCAGAGT	10420	0.0	22.063475	9
GTACTAA	515	0.0	22.04691	1
CAGAGTA	10095	0.0	21.446053	10-11
>>END_MODULE