FastQCFastQC Report
Mon 15 Aug 2016
SRR2921332_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921332_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2144792
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC21970.10243417543519373No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44600.060.3074341
GGTATCA24050.058.1460271
TCAACGC56500.046.7607574
CAACGCA58900.044.8553965
TATCAAC60300.043.9126552
GTACATG144050.043.0862121
AACGCAG62700.042.609416
TACATGG148850.041.6149062
ATCAACG63550.041.5732963
ACATGGG152850.039.9808463
CATGGGG136950.038.1052324
ATGGGGG90550.036.3398975
ACGCAGA73700.035.8402757
TGGGGGG71400.033.0840766
CGCAGAG80700.032.730698
GCAGAGT89400.028.8128349
AGAGTAC82700.024.63687310-11
ATGGGGA34200.024.53245
ATGGGGC33500.024.1568975
ATATGTA13100.023.1668071
Produced by FastQC (version 0.11.5)