##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921332_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2144792
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.518284756750305	31.0	31.0	34.0	27.0	34.0
2	30.62184165177789	33.0	31.0	34.0	27.0	34.0
3	30.798388375189763	34.0	31.0	34.0	27.0	34.0
4	34.19348169892465	37.0	35.0	37.0	31.0	37.0
5	34.2664281664609	37.0	35.0	37.0	31.0	37.0
6	34.28432966926397	37.0	35.0	37.0	31.0	37.0
7	34.243359729055314	37.0	35.0	37.0	31.0	37.0
8	34.165110183178605	37.0	35.0	37.0	30.0	37.0
9	35.78684832841599	39.0	37.0	39.0	30.0	39.0
10-11	35.788351504481554	39.0	37.0	39.0	30.0	39.0
12-13	35.6956317908683	39.0	37.0	39.0	30.0	39.0
14-15	37.005428265304985	41.0	38.0	41.0	31.0	41.0
16-17	36.9874806041798	41.0	38.0	41.0	30.0	41.0
18-19	37.001730004587856	41.0	38.0	41.0	30.0	41.0
20-21	37.031130524545034	41.0	38.0	41.0	30.0	41.0
22-23	36.98602381955919	41.0	38.0	41.0	30.0	41.0
24-25	36.96090180306528	41.0	38.0	41.0	30.0	41.0
26-27	36.91926583090574	41.0	38.0	41.0	30.0	41.0
28-29	36.8482722333914	40.0	38.0	41.0	30.0	41.0
30-31	36.768418569259865	40.0	38.0	41.0	30.0	41.0
32-33	36.672077991712015	40.0	38.0	41.0	30.0	41.0
34-35	36.564180582546	40.0	37.0	41.0	30.0	41.0
36-37	36.471172029735285	40.0	37.0	41.0	29.0	41.0
38-39	36.38394469020773	40.0	37.0	41.0	29.0	41.0
40-41	36.28165621654687	40.0	37.0	41.0	28.5	41.0
42-43	36.118062497435645	40.0	36.5	41.0	28.0	41.0
44-45	35.93185632919183	40.0	36.0	41.0	27.0	41.0
46-47	35.73796666529901	40.0	35.5	41.0	27.0	41.0
48-49	35.544771940589115	40.0	35.0	41.0	26.0	41.0
50-51	35.371760291907094	40.0	35.0	41.0	26.0	41.0
52-53	35.189989751919995	39.0	35.0	41.0	26.0	41.0
54-55	34.989661468338184	39.0	35.0	41.0	26.0	41.0
56-57	34.75070542971066	39.0	35.0	41.0	25.0	41.0
58-59	34.484281459460874	38.0	34.5	40.0	24.0	41.0
60-61	34.1943953073305	38.0	34.0	40.0	23.0	41.0
62-63	33.860782770543715	37.0	34.0	40.0	22.5	41.0
64-65	33.52705437170597	36.5	34.0	39.5	22.0	41.0
66-67	33.1882870227043	36.0	34.0	39.0	22.0	41.0
68-69	32.82428412638615	35.5	33.0	39.0	20.5	40.5
70-71	32.748988480001785	35.0	34.0	38.5	21.5	40.0
72-73	32.44098914953059	35.0	33.5	37.0	20.5	39.5
74-75	32.10326758958445	35.0	33.0	37.0	20.0	39.0
76-77	31.794077001406198	35.0	33.0	36.5	19.0	39.0
78-79	31.49981303548316	35.0	33.0	36.0	18.0	37.5
80-81	31.225439343302288	35.0	33.0	35.5	16.5	37.0
82-83	30.99784547872241	35.0	33.0	35.0	14.0	37.0
84-85	30.784262996132025	35.0	33.0	35.0	10.5	36.0
86-87	30.613346189280826	35.0	32.0	35.0	7.5	36.0
88-89	30.477836545455226	35.0	32.0	35.0	7.0	36.0
90-91	30.354700595675478	35.0	32.0	35.0	6.0	35.0
92-93	30.239031104181663	35.0	32.0	35.0	2.0	35.0
94-95	30.108980264752944	35.0	32.0	35.0	2.0	35.0
96-97	30.01106960488476	35.0	32.0	35.0	2.0	35.0
98-99	29.92092333429069	35.0	32.0	35.0	2.0	35.0
100-101	29.82108731289561	35.0	32.0	35.0	2.0	35.0
102-103	29.00797839604027	34.0	30.0	35.0	2.0	35.0
104-105	29.562207430837116	34.5	31.0	35.0	2.0	35.0
106-107	29.614949141921457	35.0	31.0	35.0	2.0	35.0
108-109	29.53588926105655	35.0	31.0	35.0	2.0	35.0
110-111	29.444451023689012	35.0	31.0	35.0	2.0	35.0
112-113	29.346225181742568	35.0	31.0	35.0	2.0	35.0
114-115	29.207169273290837	35.0	31.0	35.0	2.0	35.0
116-117	29.062197639677883	35.0	31.0	35.0	2.0	35.0
118-119	29.044573553053162	34.5	31.0	35.0	2.0	35.0
120-121	29.185513327166454	35.0	31.5	35.0	2.0	35.0
122-123	29.04169098914953	35.0	31.0	35.0	2.0	35.0
124-125	28.493742050511194	34.5	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	75017.0
3	11179.0
4	6699.0
5	5540.0
6	6129.0
7	6848.0
8	6893.0
9	7276.0
10	7914.0
11	8722.0
12	8784.0
13	8884.0
14	9252.0
15	8199.0
16	6854.0
17	6084.0
18	5809.0
19	5737.0
20	5663.0
21	5806.0
22	6776.0
23	8739.0
24	12404.0
25	16011.0
26	17487.0
27	18919.0
28	22720.0
29	27653.0
30	33789.0
31	41952.0
32	53619.0
33	71413.0
34	114475.0
35	182933.0
36	380024.0
37	717063.0
38	205440.0
39	86.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.9066414228556	17.52786301190998	12.626817507764338	24.938678057470085
2	16.209861909393837	19.66012196589096	40.03335922862496	24.096656896090245
3	19.353242762450776	25.528911727196213	28.50138374340478	26.616461766948234
4	13.619230814068711	15.399806479441356	35.207964653353386	35.772998053136554
5	14.176663438416979	38.67833250723092	32.28555881281856	14.859445241533539
6	32.6957258236405	33.87211191222088	18.52844235064542	14.903719913493205
7	29.516269977831683	32.42086041421272	20.394198746375615	17.668670861579987
8	27.86936004327309	34.76969205230075	19.719471023818848	17.641476880607314
9	29.496946990238293	15.760664551281007	18.15765607186405	36.58473238661665
10-11	26.933755724510988	25.51431713516945	26.53643508591633	21.015492054403225
12-13	26.5436922719683	22.936548532229313	26.817350361155007	23.702408834647382
14-15	24.484399504909753	23.890155989796984	24.40925937194226	27.216185133351008
16-17	24.65730526466958	26.625605622809417	24.752050759712464	23.965038352808538
18-19	24.58351226604111	26.500085561307824	25.935684007868844	22.980718164782218
20-21	25.837860244060344	25.480416725241987	25.3016017115884	23.38012131910926
22-23	25.58082769126253	24.866524158771732	25.332631751222156	24.22001639874359
24-25	24.9247435966779	24.796798385577627	25.43813308167045	24.84032493607402
26-27	24.40662919127	25.23786587936006	25.533199110736437	24.822305818633502
28-29	24.56008812575405	26.187173972594756	25.070844480192108	24.181893421459087
30-31	25.771486535602488	25.36646003857462	25.08434263567735	23.777710790145544
32-33	25.328053148760016	25.29186961618411	25.11109183809548	24.268985396960396
34-35	25.030016846736665	25.814441565417606	25.35279994814954	23.80274163969619
36-37	24.945608637712027	25.84601532563477	25.372929374692838	23.835446661960365
38-39	25.090848559467194	25.881470577469745	25.03792593677122	23.98975492629184
40-41	25.111137948142154	25.434596704702717	25.199941062149588	24.25432428500554
42-43	24.675329519787972	25.59473962014999	24.94103894553668	24.788891914525355
44-45	24.26527758899935	25.66593453016397	25.59452382723133	24.474264053605353
46-47	24.192166066008795	25.290696183378387	25.38961938232569	25.127518368287127
48-49	24.316359075267414	25.688047299754736	25.373352855051245	24.622240769926606
50-51	24.447997955863148	25.611836410301724	25.610740670886273	24.329424962948853
52-53	24.455325144987068	25.457691938261945	25.444100128970646	24.642882787780334
54-55	23.92382100748394	25.13375666393663	26.038422416239033	24.903999912340396
56-57	24.3814090249516	25.368454821153463	25.3340666370734	24.916069516821533
58-59	24.487093404953743	25.691701171292152	25.530835011936738	24.29037041181737
60-61	25.126085155301613	25.47005231312901	25.598509615530325	23.80535291603905
62-63	24.808974241509713	25.448186353947445	25.526962987105485	24.215876417437357
64-65	24.445194608516932	25.031519324703133	25.678085117097087	24.845200949682848
66-67	24.553283551817152	25.265316208989113	25.484460566837864	24.69693967235587
68-69	24.176446218468392	25.31952131195086	25.74012538622206	24.76390708335869
70-71	24.246062543756313	25.183237394859535	25.86713560767859	24.70356445370556
72-73	24.137156871773293	26.035303346975507	25.222904395608236	24.604635385642965
74-75	24.17979434011002	25.685012304716825	25.389703763592774	24.745489591580387
76-77	24.077869401851594	25.001707703221932	25.504856521456333	25.41556637347015
78-79	24.018264371792707	25.153446460660167	25.87295696132179	24.95533220622533
80-81	24.42698974134691	25.535010066553955	25.323794274886396	24.714205917212734
82-83	24.581802023427294	25.52814455130327	25.593375788813056	24.296677636456383
84-85	24.50720322149253	25.717944078978043	25.13084049396716	24.64401220556226
86-87	23.880728895035467	26.02735606174173	25.362793396664763	24.729121646558042
88-89	23.863431046778565	25.403980389702095	25.689056621016704	25.043531942502632
90-91	24.5583723445922	25.017112109965122	25.759647126844097	24.66486841859858
92-93	24.609354144775665	25.250516233284053	25.554206185100725	24.585923436839554
94-95	24.509790664655455	25.327618323778527	25.335684926876013	24.826906084690005
96-97	23.886277028673213	25.27779688263182	25.637188330211146	25.19873775848382
98-99	23.647252493361954	25.286032253830413	25.530921325189325	25.535793927618304
100-101	23.7865948464888	25.544485713173295	25.655950915210674	25.01296852512723
102-103	23.72249440766735	25.38416615085485	25.697220211430388	25.196119230047408
104-105	24.07790783554531	25.340678923927396	25.50417589877875	25.077237341748543
106-107	24.088293894573106	25.1092844323481	25.777580593043194	25.024841080035603
108-109	23.95264766306421	25.123540201972727	25.77196889370087	25.151843241262196
110-111	23.643055629059713	25.9706414942842	25.700664288473675	24.685638588182414
112-113	23.619179862324224	26.045046887024686	25.597543498984578	24.73822975166651
114-115	23.872184219741012	26.138749491197782	25.351009421641663	24.638056867419547
116-117	23.65293241762866	25.818363066381284	25.581782453542377	24.946922062447676
118-119	23.529403523126735	25.494752298102537	25.931255175196604	25.044589003574124
120-121	23.57395539736715	25.615913198025048	26.00576015727239	24.804371247335407
122-123	23.5322819751211	25.85131736829872	25.73624565479558	24.88015500178459
124-125	23.350773549712308	25.72109035272319	25.662062645369133	25.266073452195364
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	444.0
1	397.0
2	322.5
3	265.0
4	211.5
5	207.0
6	207.0
7	224.5
8	277.5
9	318.5
10	362.0
11	391.5
12	437.5
13	488.0
14	539.0
15	604.0
16	638.5
17	711.0
18	807.5
19	851.0
20	913.0
21	989.0
22	1066.5
23	1244.5
24	1514.5
25	1862.5
26	2372.0
27	3106.5
28	4157.5
29	5582.5
30	7560.5
31	9955.0
32	13260.0
33	16762.0
34	20439.5
35	24718.5
36	29274.5
37	34129.5
38	38393.5
39	43089.5
40	47770.0
41	51522.0
42	55790.5
43	60732.0
44	66206.5
45	72024.0
46	78755.5
47	86600.0
48	94922.5
49	102247.5
50	106543.5
51	108177.0
52	106059.5
53	102944.5
54	100400.0
55	96424.0
56	90239.5
57	81094.5
58	71639.5
59	61658.5
60	51833.5
61	43844.5
62	35661.0
63	27017.5
64	19708.5
65	14596.5
66	10888.5
67	8050.0
68	6042.0
69	4540.0
70	3361.0
71	2498.5
72	1804.0
73	1297.0
74	932.5
75	640.5
76	458.0
77	295.0
78	171.0
79	115.5
80	87.5
81	65.5
82	51.0
83	32.0
84	24.0
85	22.5
86	22.5
87	22.5
88	16.0
89	11.0
90	10.0
91	8.5
92	6.5
93	6.0
94	4.5
95	4.5
96	5.0
97	4.0
98	3.0
99	2.5
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01734433921797545
2	0.0120291384898862
3	0.011516268244193377
4	0.014779987989511337
5	0.008532295905616955
6	0.031844579800745246
7	0.013521124659174408
8	0.012681882438949792
9	0.008718794176777981
10-11	0.008229236214980286
12-13	0.006760562329587204
14-15	0.006177755232208998
16-17	0.004615832211235402
18-19	0.006107818380523613
20-21	0.005478386715355148
22-23	0.005385137579774635
24-25	0.0057814464059918165
26-27	0.004475958507864632
28-29	0.006037881528838228
30-31	0.005478386715355148
32-33	0.007926176524343619
34-35	0.007599804549811823
36-37	0.006993685168538487
38-39	0.0070636220202238715
40-41	0.007459930846441053
42-43	0.007273432575280027
44-45	0.0072501202913848985
46-47	0.00927828899026106
48-49	0.008019425659924133
50-51	0.0056648849865161745
52-53	0.0055016989992502765
54-55	0.006177755232208998
56-57	0.007459930846441053
58-59	0.007086934304119
60-61	0.005152014740823352
62-63	0.0056182604187259184
64-65	0.0035434671520595
66-67	0.0040563373977523225
68-69	0.006457502638950537
70-71	0.004732393630711043
72-73	0.004406021656179248
74-75	0.004009712829962066
76-77	0.004592519927340274
78-79	0.0032637197453179606
80-81	0.0030772214741569344
82-83	0.00531520072808925
84-85	0.009138415286890291
86-87	0.006923748316853102
88-89	0.0047790181985013
90-91	0.005221951592508738
92-93	0.007949488808238748
94-95	0.006853811465167717
96-97	0.009068478435204905
98-99	0.006877123749062846
100-101	0.008229236214980286
102-103	0.005338513011984379
104-105	0.004848955050186685
106-107	0.007413306278650797
108-109	0.006807186897377462
110-111	0.007762990537077721
112-113	0.005385137579774635
114-115	0.002284603821722573
116-117	0.003193782893632576
118-119	0.16640308244342575
120-121	0.010840212011234656
122-123	0.0019815441310859047
124-125	0.0017017967243443655
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2144792.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.49515916990258
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.60820523544186	46.56694636477443
2	10.903585055427333	12.756138866795858
3	3.593119119435331	6.305402244233696
4	1.66932311240963	3.905892846655939
5	0.9810787312537289	2.8694178271446473
6	0.6468786660528307	2.270356232062475
7	0.4532218443888009	1.8557898748759811
8	0.3434163322537175	1.6070554413380278
9	0.27023407767039903	1.4226646847815625
>10	1.4359115999651328	15.126275805984465
>50	0.07207868556394603	2.8220447299334395
>100	0.021992480215307034	2.0194320437070616
>500	7.958832683440491E-4	0.2975350402934959
>1k	1.5917665366879178E-4	0.17504799741878255
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2197	0.10243417543519373	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	9.324913558051317E-5	0.0
5	0.0	0.0	0.0	9.324913558051317E-5	0.0
6	0.0	0.0	0.0	9.324913558051317E-5	9.324913558051317E-5
7	0.0	0.0	0.0	9.324913558051317E-5	9.324913558051317E-5
8	0.0	0.0	0.0	9.324913558051317E-5	1.3987370337076974E-4
9	0.0	0.0	0.0	9.324913558051317E-5	1.3987370337076974E-4
10-11	0.0	0.0	0.0	9.324913558051317E-5	1.3987370337076974E-4
12-13	0.0	0.0	0.0	9.324913558051317E-5	1.8649827116102633E-4
14-15	0.0	0.0	0.0	9.324913558051317E-5	2.564351228464112E-4
16-17	0.0	0.0	0.0	9.324913558051317E-5	3.263719745317961E-4
18-19	0.0	0.0	0.0	9.324913558051317E-5	3.7299654232205267E-4
20-21	0.0	0.0	0.0	1.1656141947564145E-4	4.6624567790256585E-4
22-23	0.0	0.0	0.0	1.8649827116102633E-4	5.361825295879507E-4
24-25	0.0	0.0	0.0	5.828070973782074E-4	6.527439490635922E-4
26-27	0.0	0.0	0.0	0.0011189896269661582	6.527439490635922E-4
28-29	0.0	0.0	0.0	0.0027042249318348817	6.527439490635922E-4
30-31	0.0	0.0	0.0	0.010094218926590551	6.527439490635922E-4
32-33	0.0	0.0	0.0	0.03046915505093268	6.993685168538488E-4
34-35	0.0	0.0	0.0	0.05697522183969355	6.993685168538488E-4
36-37	0.0	0.0	0.0	0.09406506551684266	6.993685168538488E-4
38-39	0.0	0.0	0.0	0.1617406256644001	8.159299363294902E-4
40-41	0.0	0.0	0.0	0.24820588663143092	8.392422202246185E-4
42-43	0.0	0.0	0.0	0.32763083786213304	8.392422202246185E-4
44-45	0.0	0.0	0.0	0.40449143786437103	8.392422202246185E-4
46-47	0.0	0.0	0.0	0.48613105606511026	9.091790719100034E-4
48-49	0.0	0.0	0.0	0.5811286129377581	9.791159235953883E-4
50-51	0.0	0.0	0.0	0.6887381153976703	0.0010024282074905166
52-53	0.0	0.0	0.0	0.8068614578942853	0.0010490527752807731
54-55	0.0	0.0	0.0	0.9297638185894017	0.0010956773430710297
56-57	0.0	0.0	0.0	1.0495190209586758	0.001118989626966158
58-59	0.0	0.0	0.0	1.1894160366133406	0.001118989626966158
60-61	0.0	0.0	0.0	1.3364232988560196	0.0011656141947564147
62-63	0.0	0.0	0.0	1.5090274488155495	0.0012588633303369278
64-65	0.0	0.0	0.0	1.6639142630147818	0.001282175614232056
66-67	0.0	0.0	0.0	1.8310167139750613	0.0013288001820223126
68-69	0.0	0.0	0.0	2.0169788026065	0.0013521124659174408
70-71	0.0	0.0	0.0	2.218863181138311	0.0015386107370784672
72-73	0.0	0.0	0.0	2.4092779159937185	0.001678484440449237
74-75	0.0	0.0	0.0	2.6067329605854557	0.001678484440449237
76-77	0.0	0.0	0.0	2.8399723609562137	0.001678484440449237
78-79	0.0	0.0	0.0	3.0975264734295913	0.001678484440449237
80-81	0.0	0.0	0.0	3.3392748574220716	0.0017017967243443653
82-83	0.0	0.0	0.0	3.599066949149381	0.0017251090082394936
84-85	0.0	0.0	0.0	3.877928489102906	0.0017484212921346218
86-87	0.0	0.0	0.0	4.184181962633207	0.00177173357602975
88-89	0.0	0.0	0.0	4.519435917329046	0.0017950458599248786
90-91	0.0	0.0	0.0	4.878211966475071	0.0018649827116102634
92-93	0.0	0.0	0.0	5.215377528450311	0.0018649827116102634
94-95	0.0	0.0	0.0	5.563942797250269	0.0019582318471907767
96-97	0.0	0.0	0.0	5.950040843121384	0.0020281686988761613
98-99	0.0	0.0	0.0	6.384581814926576	0.0020981055505615463
100-101	0.0	0.0	0.0	6.84423011648682	0.0020981055505615463
102-103	0.0	4.6624567790256583E-5	0.0	7.334137762542941	0.002144730118351803
104-105	0.0	4.6624567790256583E-5	0.0	7.85894389759007	0.0022146669700371874
106-107	0.0	4.6624567790256583E-5	0.0	8.418601897060414	0.002237979253932316
108-109	0.0	4.6624567790256583E-5	0.0	8.97935557387383	0.002307916105617701
110-111	4.6624567790256583E-5	4.6624567790256583E-5	0.0	9.573072820114959	0.0024244775250933425
112-113	9.324913558051317E-5	4.6624567790256583E-5	0.0	10.171895456529118	0.002471102092883599
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4460	0.0	60.307434	1
GGTATCA	2405	0.0	58.146027	1
TCAACGC	5650	0.0	46.760757	4
CAACGCA	5890	0.0	44.855396	5
TATCAAC	6030	0.0	43.912655	2
GTACATG	14405	0.0	43.086212	1
AACGCAG	6270	0.0	42.60941	6
TACATGG	14885	0.0	41.614906	2
ATCAACG	6355	0.0	41.573296	3
ACATGGG	15285	0.0	39.980846	3
CATGGGG	13695	0.0	38.105232	4
ATGGGGG	9055	0.0	36.339897	5
ACGCAGA	7370	0.0	35.840275	7
TGGGGGG	7140	0.0	33.084076	6
CGCAGAG	8070	0.0	32.73069	8
GCAGAGT	8940	0.0	28.812834	9
AGAGTAC	8270	0.0	24.636873	10-11
ATGGGGA	3420	0.0	24.5324	5
ATGGGGC	3350	0.0	24.156897	5
ATATGTA	1310	0.0	23.166807	1
>>END_MODULE