FastQCFastQC Report
Mon 15 Aug 2016
SRR2921332_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921332_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2144792
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27200.1268188243894979No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23060.10751625332433168No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22990.1071898813497999No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29100.065.937931
GTATCAA52300.055.0894741
GTACATG135750.043.2849541
TACATGG142350.041.3279152
ACATGGG146600.039.6825643
CATGGGG130350.038.6393244
GTAAGTA15100.038.2894062
ATGGGGG88450.037.1310845
TATCAAC77150.036.775192
TCAACGC74950.036.582224
CAACGCA77350.035.3700945
TGTAAGT16350.034.7319451
TGGGGGG67600.034.5590976
ATCAACG80350.034.2720383
AACGCAG79750.034.0019386
ACGCAGA95400.028.1656237
TAAGTAC22350.027.2023563
CGCAGAG99000.026.9564678
AGAGTAC102900.024.25456410-11
CAGAGTA106900.023.125479
Produced by FastQC (version 0.11.5)