##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921332_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2144792
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.926919719954196	31.0	30.0	34.0	16.0	34.0
2	29.712525969884258	33.0	31.0	34.0	25.0	34.0
3	29.974666541091164	33.0	31.0	34.0	26.0	34.0
4	32.10744771520968	37.0	33.0	37.0	19.0	37.0
5	31.723025356304948	37.0	33.0	37.0	19.0	37.0
6	31.875966061044615	37.0	33.0	37.0	17.0	37.0
7	32.09862588073808	37.0	35.0	37.0	17.0	37.0
8	32.355157516439824	37.0	35.0	37.0	17.0	37.0
9	34.063654657421324	39.0	35.0	39.0	17.0	39.0
10-11	34.270747000175305	39.0	37.0	39.0	17.0	39.0
12-13	34.26320314510684	39.0	37.0	39.0	17.5	39.0
14-15	35.63955595694128	40.0	37.0	41.0	19.0	41.0
16-17	35.64976230795341	40.0	37.0	41.0	20.5	41.0
18-19	35.68235940827829	40.5	38.0	41.0	21.0	41.0
20-21	35.66889726369736	40.0	38.0	41.0	19.5	41.0
22-23	35.61938593579237	40.0	37.0	41.0	19.0	41.0
24-25	35.554198728827785	40.5	37.0	41.0	18.5	41.0
26-27	35.46114891327457	40.0	37.0	41.0	18.0	41.0
28-29	35.383651887922	40.0	37.0	41.0	17.5	41.0
30-31	35.27892937869966	40.0	36.5	41.0	16.5	41.0
32-33	35.14274554362381	40.0	36.0	41.0	15.5	41.0
34-35	35.06409852330668	40.0	36.0	41.0	14.5	41.0
36-37	34.988624071704855	40.0	35.5	41.0	9.5	41.0
38-39	34.89072739920701	40.0	35.0	41.0	9.0	41.0
40-41	34.75699321892286	40.0	35.0	41.0	8.0	41.0
42-43	34.61862642158307	40.0	35.0	41.0	4.0	41.0
44-45	34.47297919798284	40.0	35.0	41.0	2.0	41.0
46-47	34.26119828869186	40.0	35.0	41.0	2.0	41.0
48-49	34.05603247307897	40.0	35.0	41.0	2.0	41.0
50-51	33.8742614668462	39.5	35.0	41.0	2.0	41.0
52-53	33.69690370907762	39.0	35.0	41.0	2.0	41.0
54-55	33.4966514235413	39.0	34.5	41.0	2.0	41.0
56-57	33.258472849581686	38.5	34.0	41.0	2.0	41.0
58-59	33.192883505719905	38.5	34.0	41.0	2.0	41.0
60-61	32.951957345980404	37.5	34.0	41.0	2.0	41.0
62-63	32.66371750733871	37.0	34.0	40.0	2.0	41.0
64-65	32.371492900010814	36.5	34.0	40.0	2.0	41.0
66-67	32.058882166662315	36.0	33.5	39.0	2.0	41.0
68-69	31.72218401597917	35.5	33.0	39.0	2.0	41.0
70-71	31.394296276748513	35.0	33.0	38.5	2.0	40.5
72-73	31.048841332865845	35.0	33.0	37.0	2.0	39.5
74-75	30.713937062428432	35.0	33.0	37.0	2.0	39.0
76-77	30.41948333451449	35.0	32.0	36.0	2.0	39.0
78-79	30.16168047997195	35.0	32.0	36.0	2.0	37.0
80-81	29.938033151932682	35.0	32.0	35.5	2.0	37.0
82-83	29.738662303850443	35.0	32.0	35.0	2.0	36.5
84-85	29.572351771174084	35.0	32.0	35.0	2.0	36.0
86-87	29.437524711020927	35.0	32.0	35.0	2.0	36.0
88-89	29.320680047295962	35.0	32.0	35.0	2.0	36.0
90-91	29.223144248952813	35.0	31.0	35.0	2.0	35.0
92-93	29.154132894938066	35.0	31.0	35.0	2.0	35.0
94-95	29.08571926788239	35.0	31.0	35.0	2.0	35.0
96-97	29.02187834531274	35.0	31.0	35.0	2.0	35.0
98-99	28.97064680397913	35.0	31.0	35.0	2.0	35.0
100-101	28.915977167016663	35.0	31.0	35.0	2.0	35.0
102-103	28.244930277621325	34.0	29.5	35.0	2.0	35.0
104-105	28.78267915956419	35.0	31.0	35.0	2.0	35.0
106-107	28.836833828175415	35.0	31.0	35.0	2.0	35.0
108-109	28.905478946210167	35.0	31.5	35.0	2.0	35.0
110-111	29.015988729909473	35.0	32.0	35.0	2.0	35.0
112-113	28.977209678141286	35.0	32.0	35.0	2.0	35.0
114-115	28.91408141209031	35.0	32.0	35.0	2.0	35.0
116-117	28.844371622050062	35.0	32.0	35.0	2.0	35.0
118-119	28.7621818339494	35.0	31.5	35.0	2.0	35.0
120-121	28.65988240351512	35.0	31.0	35.0	2.0	35.0
122-123	28.539077682124883	35.0	31.0	35.0	2.0	35.0
124-125	28.015562581359873	34.5	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	159386.0
3	10209.0
4	7368.0
5	6851.0
6	7151.0
7	7445.0
8	7660.0
9	7662.0
10	7929.0
11	7949.0
12	8718.0
13	10262.0
14	12201.0
15	10333.0
16	7219.0
17	6182.0
18	5671.0
19	5033.0
20	4941.0
21	5051.0
22	5785.0
23	7116.0
24	9262.0
25	11818.0
26	14163.0
27	16254.0
28	19346.0
29	23174.0
30	27827.0
31	34678.0
32	44657.0
33	60196.0
34	94597.0
35	159547.0
36	335656.0
37	728060.0
38	247162.0
39	273.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.98569339650231	17.779119143709423	12.235316137334035	26.999871322454233
2	15.438606634116503	20.008933267188613	38.62043498856765	25.932025110127228
3	18.399220064230004	24.940413802364052	28.880516152615265	27.779849980790676
4	13.801471640904323	16.493635309309	31.676046873520864	38.028846176265816
5	13.917304260904494	38.347533234307704	31.277944377493156	16.45721812729464
6	32.56190181636506	34.364318878263845	17.604984332994196	15.468794972376902
7	29.265248787913272	32.36999098979776	19.661641722273107	18.703118500015854
8	28.170349238974428	35.29119101597216	18.20805313423538	18.330406610818024
9	29.04092483630112	16.079722508077115	17.757953669591945	37.12139898602982
10-11	27.145884150499395	25.449034719601144	25.53895239958046	21.866128730318994
12-13	26.64089656082916	22.98897306314842	26.1412858648357	24.22884451118672
14-15	24.61641968078956	23.948662620897505	24.143273566854035	27.291644131458902
16-17	24.31776601180907	26.77432123954211	24.407821364495952	24.50009138415287
18-19	24.45003991063003	26.098661315409604	26.032780801121973	23.418517972838394
20-21	25.51848850611155	25.041332679346063	25.682303925042614	23.757874889499774
22-23	25.28692759018124	24.433208441657747	25.541544354883833	24.738319613277184
24-25	24.685587227106403	24.702908254040484	25.609639536141497	25.00186498271161
26-27	24.120101156662276	24.969297722110113	25.860456398569188	25.05014472265842
28-29	24.407867989063742	25.73939570830178	25.43106744150482	24.421668861129657
30-31	25.469789145054627	25.1019213051895	25.46419419691979	23.96409535283608
32-33	24.993868869335582	25.225406472981998	25.473146113935524	24.3075785437469
34-35	24.869148150496645	25.623626906478574	25.66426021730779	23.842964725716993
36-37	24.890712013099638	25.413723102286838	25.833717208941476	23.861847675672045
38-39	24.840777100996274	25.646286446424643	25.499978552698817	24.01295789988027
40-41	24.81984267005845	25.41302373376999	25.530983890279334	24.236149705892228
42-43	24.457406592340888	25.561826041872592	25.200415704646417	24.780351661140102
44-45	24.037762169944685	25.616283537051608	25.885283980917496	24.460670312086208
46-47	23.90919492426305	25.42817671830182	25.392788671349013	25.269839686086108
48-49	24.150174002886992	25.78147438073249	25.28571535141869	24.782636264961823
50-51	24.269999142107952	25.715780364716018	25.511518133226907	24.502702359949126
52-53	24.37322593519558	25.498789626220166	25.42813009373403	24.699854344850223
54-55	23.74190597503161	25.125979582169272	26.064695317774405	25.06741912502471
56-57	24.138541173223324	25.418222373078603	25.30271000637824	25.140526447319832
58-59	24.29007101854166	25.66931898291303	25.48039623422691	24.560213764318405
60-61	24.890828574519112	25.424703188001445	25.679623012394675	24.004845225084765
62-63	24.528485745937132	25.416520576354255	25.63509655015498	24.41989712755363
64-65	24.212138053480246	24.94670811901574	25.690276726134748	25.150877101369268
66-67	24.194187594880994	25.335440452967	25.47925393231605	24.991118019835955
68-69	23.844060403060062	25.324600240955768	25.744337912487552	25.08700144349662
70-71	23.969527114983645	25.217130612199224	25.771636596928747	25.041705675888387
72-73	23.88336491370725	26.000563224778904	25.1124584575101	25.003613404003744
74-75	23.8572784680286	25.65913151485086	25.34707328263067	25.136516734489874
76-77	23.703813703146974	25.04958522784494	25.421183033133282	25.825418035874808
78-79	23.675069657104277	25.226851834583492	25.77343164278867	25.324646865523558
80-81	24.10352612281284	25.53853707026136	25.130478852961037	25.227457953964766
82-83	24.11961159870048	25.6627682311385	25.311032491728803	24.90658767843222
84-85	24.018063290053302	25.820382582553457	24.934632355958062	25.22692177143518
86-87	23.477451426525274	26.143747272462786	25.06567070373258	25.313130597279365
88-89	23.36958548894252	25.639292761256105	25.399968854788717	25.591152895012666
90-91	24.033657405766913	25.209414636784615	25.444472833678667	25.312455123769805
92-93	24.026563949372118	25.498901638927695	25.285523757259753	25.18901065444043
94-95	23.95112157781341	25.50393021977433	25.015071398565176	25.529876803847092
96-97	23.342357545777887	25.416947289134505	25.235623795479782	26.005071369607823
98-99	23.091825009563866	25.43892961157297	25.229235569051816	26.240009809811347
100-101	23.26454033771107	25.70650207572576	25.270119433492848	25.758838153070325
102-103	23.124433511501348	25.523827019123534	25.25771729846064	26.094022170914478
104-105	23.31571623628684	25.579362842495414	25.123700835256013	25.981220085961738
106-107	23.47981002331464	25.231916698585106	25.330574399026105	25.957698879074147
108-109	23.33567234011801	25.217585911874618	25.34291315912784	26.10382858887954
110-111	23.02164411740845	26.10397960449201	25.20514867217522	25.669227605924323
112-113	22.98718095765622	26.173175032567475	25.126773027324973	25.71287098245133
114-115	23.085934194382517	26.17169587742183	24.938449584609003	25.803920343586643
116-117	22.8463185751832	25.834828824015133	25.157650128381405	26.161202472420257
118-119	22.7574706684297	25.599429873721785	25.392229908037535	26.250869549810986
120-121	22.782119177662892	25.70767434286675	25.499122408049836	26.011084071420527
122-123	22.67428211748387	25.909255686818472	25.154393551733584	26.262068643964078
124-125	22.382926821307997	25.902314025002905	24.917346258912872	26.79741289477623
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	66.0
1	57.0
2	89.5
3	110.5
4	95.0
5	153.0
6	283.5
7	660.0
8	987.0
9	1111.5
10	1034.5
11	852.0
12	878.0
13	958.5
14	1008.0
15	1111.5
16	1230.5
17	1282.5
18	1349.5
19	1450.0
20	1580.0
21	1688.5
22	1807.5
23	1992.0
24	2208.5
25	2561.5
26	3133.0
27	3827.5
28	4711.0
29	5984.5
30	7645.0
31	9702.0
32	12657.0
33	15957.5
34	19323.5
35	22749.5
36	26932.0
37	31861.5
38	35925.0
39	40359.0
40	45299.0
41	49183.0
42	52989.5
43	57893.5
44	63377.0
45	69097.0
46	75678.5
47	83039.0
48	91875.0
49	100540.0
50	106174.0
51	109237.5
52	108362.0
53	105542.5
54	102769.5
55	98668.0
56	91826.0
57	82763.5
58	73398.5
59	63244.5
60	53430.5
61	45082.0
62	36947.5
63	28154.0
64	20435.0
65	15121.5
66	11312.5
67	8418.0
68	6395.5
69	4835.0
70	3664.0
71	2806.0
72	2064.0
73	1565.5
74	1213.5
75	888.5
76	668.5
77	471.0
78	345.5
79	283.0
80	239.5
81	202.5
82	196.5
83	192.0
84	158.0
85	148.0
86	154.5
87	151.5
88	122.0
89	108.5
90	121.0
91	109.5
92	101.5
93	92.0
94	72.5
95	72.0
96	63.5
97	39.0
98	27.0
99	17.0
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3574705612478972
2	0.0
3	0.0
4	0.01785720946366827
5	0.1833278005512889
6	0.0519863930861361
7	0.02592325969138266
8	0.008672169608987725
9	0.007133558871909258
10-11	0.0024477898089884705
12-13	0.057021846407483805
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	1.8649827116102633E-4
92-93	2.564351228464112E-4
94-95	4.6624567790256583E-5
96-97	3.030596906366678E-4
98-99	2.3312283895128292E-5
100-101	0.0
102-103	0.0
104-105	2.3312283895128292E-5
106-107	1.1656141947564145E-4
108-109	3.2637197453179613E-4
110-111	2.797474067415395E-4
112-113	6.527439490635923E-4
114-115	2.564351228464112E-4
116-117	5.128702456928224E-4
118-119	1.8649827116102633E-4
120-121	1.1656141947564145E-4
122-123	0.0013754247498125691
124-125	4.6624567790256583E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2144792.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.59364236897892
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.43801776207877	50.77657783645012
2	9.279869696301244	11.431619506094078
3	3.1032727432020577	5.734256145545631
4	1.4998241166842317	3.6951852103767306
5	0.8452724798484029	2.6031705414061235
6	0.5716796610414658	2.1127099555084285
7	0.39136430382486875	1.6873887076041365
8	0.30845886339538797	1.51992839340138
9	0.21515628203346462	1.1927033180107547
>10	1.2627166648880304	13.951700072866963
>50	0.06318818344673856	2.6117267370325723
>100	0.020348109698394613	2.0136678057715796
>500	4.5334557650781584E-4	0.16335075105752822
>1k	3.777879804229927E-4	0.5060150188739997
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2720	0.1268188243894979	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2306	0.10751625332433168	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2299	0.1071898813497999	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	4.6624567790256583E-5
10-11	0.0	0.0	0.0	0.0	4.6624567790256583E-5
12-13	0.0	0.0	0.0	0.0	4.6624567790256583E-5
14-15	0.0	0.0	0.0	0.0	1.1656141947564146E-4
16-17	0.0	0.0	0.0	0.0	2.3312283895128292E-4
18-19	0.0	0.0	0.0	0.0	2.3312283895128292E-4
20-21	0.0	0.0	0.0	2.3312283895128292E-5	2.797474067415395E-4
22-23	0.0	0.0	0.0	9.324913558051317E-5	4.1962111011230926E-4
24-25	0.0	0.0	0.0	4.895579617976941E-4	5.361825295879507E-4
26-27	0.0	0.0	0.0	0.0010024282074905166	5.59494813483079E-4
28-29	0.0	0.0	0.0	0.0026809126479397536	5.59494813483079E-4
30-31	0.0	0.0	0.0	0.010047594358800293	5.59494813483079E-4
32-33	0.0	0.0	0.0	0.029676537398498314	6.527439490635922E-4
34-35	0.0	0.0	0.0	0.05499367770860764	6.993685168538488E-4
36-37	0.0	0.0	0.0	0.08947254558950238	6.993685168538488E-4
38-39	0.0	0.0	0.0	0.15402425969511263	6.993685168538488E-4
40-41	0.0	0.0	0.0	0.23552400419248112	6.993685168538488E-4
42-43	0.0	0.0	0.0	0.30954050555951346	6.993685168538488E-4
44-45	0.0	0.0	0.0	0.38180858563441117	6.993685168538488E-4
46-47	0.0	0.0	0.0	0.45857593650106865	7.226808007489771E-4
48-49	0.0	0.0	0.0	0.5481883557939418	7.92617652434362E-4
50-51	0.0	0.0	0.0	0.6498765381444914	7.92617652434362E-4
52-53	0.0	0.0	0.0	0.7620319359639536	8.858667880148752E-4
54-55	0.0	0.0	0.0	0.8778939869227412	9.791159235953883E-4
56-57	0.0	4.6624567790256583E-5	0.0	0.9920542411571844	9.791159235953883E-4
58-59	0.0	4.6624567790256583E-5	0.0	1.1245845751009886	9.791159235953883E-4
60-61	0.0	4.6624567790256583E-5	0.0	1.264178531065017	9.791159235953883E-4
62-63	0.0	4.6624567790256583E-5	0.0	1.4308846731990794	0.0011656141947564147
64-65	0.0	6.993685168538487E-5	0.0	1.577985184577339	0.0011656141947564147
66-67	0.0	9.324913558051317E-5	0.0	1.7350633534627133	0.0011656141947564147
68-69	0.0	9.324913558051317E-5	0.0	1.9103484160701831	0.0011656141947564147
70-71	0.0	9.324913558051317E-5	0.0	2.102581509069411	0.0011656141947564147
72-73	0.0	9.324913558051317E-5	0.0	2.2819229090746327	0.0013054878981271843
74-75	0.0	9.324913558051317E-5	0.0	2.4672322537570075	0.0013054878981271843
76-77	0.0	1.1656141947564145E-4	0.0	2.687090403171963	0.0013054878981271843
78-79	0.0	1.3987370337076974E-4	0.0	2.9284191660543306	0.0013054878981271843
80-81	0.0	1.3987370337076974E-4	0.0	3.15356920391348	0.0013754247498125693
82-83	0.0	1.3987370337076974E-4	0.0	3.3956439598804917	0.001585235304868724
84-85	0.0	1.3987370337076974E-4	0.0	3.6564617920991873	0.001585235304868724
86-87	4.6624567790256583E-5	1.3987370337076974E-4	0.0	3.945720610669939	0.0016085475887638522
88-89	4.6624567790256583E-5	1.3987370337076974E-4	0.0	4.26129899775829	0.0016318598726589805
90-91	4.6624567790256583E-5	1.3987370337076974E-4	0.0	4.595340713691584	0.0016318598726589805
92-93	4.6624567790256583E-5	1.3987370337076974E-4	0.0	4.9118982167035306	0.0016318598726589805
94-95	4.6624567790256583E-5	1.3987370337076974E-4	0.0	5.241720409251807	0.0018183581438200069
96-97	4.6624567790256583E-5	1.3987370337076974E-4	0.0	5.606394466223298	0.0018183581438200069
98-99	4.6624567790256583E-5	1.3987370337076974E-4	0.0	6.0183924595019	0.0018183581438200069
100-101	4.6624567790256583E-5	1.3987370337076974E-4	0.0	6.453119929578253	0.0018416704277151351
102-103	4.6624567790256583E-5	1.3987370337076974E-4	0.0	6.915239333231381	0.0018649827116102634
104-105	4.6624567790256583E-5	1.3987370337076974E-4	0.0	7.409226628969149	0.0019349195632956482
106-107	4.6624567790256583E-5	1.3987370337076974E-4	0.0	7.941026449184816	0.002004856414981033
108-109	4.6624567790256583E-5	1.3987370337076974E-4	0.0	8.473245890510595	0.0020281686988761613
110-111	4.6624567790256583E-5	1.3987370337076974E-4	0.0	9.033043763684312	0.0021214178344566743
112-113	4.6624567790256583E-5	1.3987370337076974E-4	0.0	9.60335547689473	0.002144730118351803
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2910	0.0	65.93793	1
GTATCAA	5230	0.0	55.089474	1
GTACATG	13575	0.0	43.284954	1
TACATGG	14235	0.0	41.327915	2
ACATGGG	14660	0.0	39.682564	3
CATGGGG	13035	0.0	38.639324	4
GTAAGTA	1510	0.0	38.289406	2
ATGGGGG	8845	0.0	37.131084	5
TATCAAC	7715	0.0	36.77519	2
TCAACGC	7495	0.0	36.58222	4
CAACGCA	7735	0.0	35.370094	5
TGTAAGT	1635	0.0	34.731945	1
TGGGGGG	6760	0.0	34.559097	6
ATCAACG	8035	0.0	34.272038	3
AACGCAG	7975	0.0	34.001938	6
ACGCAGA	9540	0.0	28.165623	7
TAAGTAC	2235	0.0	27.202356	3
CGCAGAG	9900	0.0	26.956467	8
AGAGTAC	10290	0.0	24.254564	10-11
CAGAGTA	10690	0.0	23.12547	9
>>END_MODULE