FastQCFastQC Report
Mon 15 Aug 2016
SRR2921331_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921331_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2157537
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19350.057.2111131
GTATCAA34100.053.234591
TCAACGC44850.039.411444
CAACGCA45500.038.7176175
ATCAACG47900.036.777693
TATCAAC49000.036.4379122
AACGCAG48500.036.1974836
GTACATG95650.035.9037061
TACATGG99000.034.446712
ACATGGG102900.032.562773
CATGGGG91700.029.9847854
ATGGGGG57450.028.5921845
ACGCAGA61150.028.3102447
CGCAGAG64850.026.7867478
GTGGTAT12100.025.0861051
GCAGAGT74350.022.802949
AGAGTAC66800.021.68450210-11
TGGTATC12800.021.3882942
TGGGGGG44850.019.5053166
GAGTACA68650.019.2469041
Produced by FastQC (version 0.11.5)