##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921331_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2157537
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.390750193391817	31.0	31.0	34.0	26.0	34.0
2	30.490721595967994	33.0	31.0	34.0	26.0	34.0
3	30.473964061798245	33.0	31.0	34.0	26.0	34.0
4	33.83772885470794	37.0	35.0	37.0	30.0	37.0
5	33.91614095146456	37.0	35.0	37.0	30.0	37.0
6	33.9814876871173	37.0	35.0	37.0	30.0	37.0
7	33.880423371650174	37.0	35.0	37.0	30.0	37.0
8	33.89711648050532	37.0	35.0	37.0	30.0	37.0
9	35.48631889047557	39.0	37.0	39.0	30.0	39.0
10-11	35.51041697083295	39.0	37.0	39.0	30.0	39.0
12-13	35.490013380998796	39.0	37.0	39.0	30.0	39.0
14-15	36.82221741736063	40.5	38.0	41.0	30.0	41.0
16-17	36.82835080000946	41.0	38.0	41.0	30.0	41.0
18-19	36.823019721098646	41.0	38.0	41.0	30.0	41.0
20-21	36.834812798111926	41.0	38.0	41.0	30.0	41.0
22-23	36.801980684456396	40.5	38.0	41.0	30.0	41.0
24-25	36.794313840272494	41.0	38.0	41.0	30.0	41.0
26-27	36.75598471775918	40.0	38.0	41.0	30.0	41.0
28-29	36.68386173678597	40.0	38.0	41.0	30.0	41.0
30-31	36.6143285144125	40.0	38.0	41.0	30.0	41.0
32-33	36.53127223310655	40.0	38.0	41.0	30.0	41.0
34-35	36.423737808436194	40.0	37.5	41.0	29.0	41.0
36-37	36.31349311738339	40.0	37.0	41.0	28.0	41.0
38-39	36.21012663977489	40.0	37.0	41.0	28.0	41.0
40-41	36.106428302272455	40.0	37.0	41.0	27.0	41.0
42-43	35.93276847627642	40.0	36.5	41.0	27.0	41.0
44-45	35.71274606182884	40.0	36.0	41.0	26.0	41.0
46-47	35.49627051587065	40.0	35.5	41.0	25.5	41.0
48-49	35.29848619050334	40.0	35.0	41.0	24.5	41.0
50-51	35.13644563221859	39.5	35.0	41.0	24.0	41.0
52-53	34.95493935909326	39.0	35.0	41.0	24.0	41.0
54-55	34.76023562979453	39.0	35.0	41.0	23.0	41.0
56-57	34.533834182217966	39.0	35.0	41.0	23.0	41.0
58-59	34.281454501127904	38.0	34.0	40.0	22.0	41.0
60-61	34.01289711369955	38.0	34.0	40.0	22.0	41.0
62-63	33.70992154479853	37.0	34.0	40.0	20.5	41.0
64-65	33.38166182086333	37.0	34.0	40.0	19.5	41.0
66-67	33.047911808696675	36.0	33.0	39.0	18.5	41.0
68-69	32.704474361273995	36.0	33.0	39.0	18.0	40.5
70-71	32.65425784123285	35.5	33.0	39.0	18.0	40.0
72-73	32.36421692884062	35.0	33.0	38.0	17.0	40.0
74-75	32.03051535153279	35.0	33.0	37.0	14.0	39.0
76-77	31.71606535600548	35.0	33.0	37.0	12.5	39.0
78-79	31.412067788408727	35.0	33.0	36.0	9.5	39.0
80-81	31.11586336642199	35.0	33.0	36.0	7.0	37.0
82-83	30.871884468261726	35.0	32.5	35.5	7.0	37.0
84-85	30.63965948208536	35.0	32.0	35.0	2.0	36.5
86-87	30.45823316123895	35.0	32.0	35.0	2.0	36.0
88-89	30.306130787096585	35.0	32.0	35.0	2.0	36.0
90-91	30.17147075577383	35.0	32.0	35.0	2.0	36.0
92-93	30.052491104439923	35.0	32.0	35.0	2.0	35.0
94-95	29.92663671584775	35.0	32.0	35.0	2.0	35.0
96-97	29.83339358722469	35.0	31.5	35.0	2.0	35.0
98-99	29.75685770394668	35.0	31.0	35.0	2.0	35.0
100-101	29.6640393652577	35.0	31.0	35.0	2.0	35.0
102-103	28.85523608633363	33.5	29.5	35.0	2.0	35.0
104-105	29.43655103017932	34.0	31.0	35.0	2.0	35.0
106-107	29.512547872875416	35.0	31.0	35.0	2.0	35.0
108-109	29.45590203088058	35.0	31.0	35.0	2.0	35.0
110-111	29.378269990271313	35.0	31.0	35.0	2.0	35.0
112-113	29.29166660873023	34.5	31.0	35.0	2.0	35.0
114-115	29.173841978144523	34.0	31.0	35.0	2.0	35.0
116-117	29.044909774432604	34.0	31.0	35.0	2.0	35.0
118-119	29.046151931577533	34.5	30.5	35.0	2.0	35.0
120-121	29.220358445764777	35.0	31.0	35.0	2.0	35.0
122-123	29.09439189223638	35.0	31.0	35.0	2.0	35.0
124-125	28.541097788821233	34.5	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	84762.0
3	11169.0
4	7230.0
5	6114.0
6	6619.0
7	7330.0
8	7385.0
9	7561.0
10	8333.0
11	8855.0
12	9258.0
13	8975.0
14	9787.0
15	9010.0
16	7169.0
17	6704.0
18	6037.0
19	5711.0
20	5769.0
21	6036.0
22	6706.0
23	8674.0
24	12153.0
25	15044.0
26	16194.0
27	18222.0
28	21488.0
29	26271.0
30	31348.0
31	39509.0
32	51167.0
33	70219.0
34	108840.0
35	183489.0
36	386802.0
37	706553.0
38	224921.0
39	123.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.54169544575054	18.570041877623144	12.999956425762942	24.888306250863373
2	15.871144065518205	19.51746701005505	39.97985467180637	24.631534252620376
3	19.532709413951505	24.119490295874993	28.58950647327947	27.75829381689403
4	13.63255410773802	15.419673939747922	35.54566651369555	35.4021054388185
5	14.250837244336289	38.243374471290345	33.19399733472391	14.311790949649458
6	33.88355456235598	35.14444408384294	16.70261348515687	14.269387868644207
7	29.236092923215274	32.671832157848016	19.75969572583804	18.332379193098667
8	26.935820914894528	35.98468624320671	18.83194361778465	18.247549224114117
9	27.164678195918796	16.59834992233538	18.64599812826181	37.590973753484015
10-11	26.001802654941297	26.25266325001211	26.57650610800587	21.16902798704072
12-13	25.87968552026289	23.575643788635837	26.997927370597402	23.546743320503875
14-15	23.404643884495265	25.004264356744958	24.220572925599026	27.37051883316075
16-17	23.73854327252434	28.07527441915655	24.865304945709457	23.320877362609654
18-19	23.74288993999078	27.404001588009002	26.3380748797695	22.515033592230715
20-21	24.594136844657022	26.147042859612718	26.241900698003512	23.016919597726748
22-23	24.46511763727052	25.754703381836876	26.0931837175315	23.68699526336111
24-25	24.042182577333875	25.950192613344296	26.168971336742636	23.838653472579193
26-27	23.789489199552992	26.33007285892604	26.17690642270043	23.703531518820537
28-29	23.690539344482275	26.632148656882315	26.11817933837314	23.55913266026227
30-31	24.245824738074926	26.164024530004205	26.052758373989164	23.537392357931704
32-33	23.856805675042274	26.168247908570414	26.087269905663174	23.88767651072414
34-35	23.96536539691663	26.325913839935478	26.28584208623423	23.422878676913665
36-37	23.957662917785974	26.39182033740158	26.169214371283577	23.48130237352887
38-39	23.952347413433674	26.55765151351866	26.08031912391105	23.409681949136612
40-41	24.016206533647075	26.337957955438522	26.060865351210705	23.5849701597037
42-43	23.646996411643766	26.34043055176047	26.102272903727158	23.910300132868606
44-45	23.817693940003544	26.282681791086837	26.213014405342744	23.686609863566876
46-47	23.740804107263127	26.227884315426014	26.111653700907922	23.919657876402937
48-49	23.551980020181915	26.536414358732852	26.160216781767858	23.751388839317375
50-51	23.70355125908951	26.439187886338942	26.1396187689809	23.717642085590647
52-53	23.798560657556038	26.32523169204663	26.108655916134072	23.76755173426326
54-55	23.512434771564898	26.184749547839132	26.2780787033389	24.02473697725707
56-57	23.659581549152538	26.317659426732163	25.95219399773615	24.070565026379146
58-59	23.73008627744362	26.435541291475552	26.107090454461286	23.727281976619548
60-61	24.02563120872438	26.296346424108485	26.122460425465643	23.55556194170149
62-63	23.746165870774636	26.272594855227222	26.2205190660658	23.760720207932344
64-65	23.60434236784332	26.09921313528497	26.19742927707775	24.09901521979396
66-67	23.71638453457417	26.387359641765666	25.970830192116185	23.925425631543977
68-69	23.605563700598818	26.32778239493319	26.068884353621673	23.997769550846314
70-71	23.578921363115654	26.2103325933409	26.210100838743067	24.00064520480038
72-73	23.719777934082437	26.466493701527373	25.93040219609334	23.883326168296858
74-75	23.597661434550968	26.42677683858897	26.031662453797296	23.943899273062765
76-77	23.512308330878675	26.135275183357848	26.09342043878883	24.25899604697465
78-79	23.50525240464837	26.129044518439965	26.178361131093197	24.18734194581847
80-81	23.71145655971109	26.448185375144064	25.880973884169272	23.959384180975576
82-83	23.683139569916595	26.429725573155615	26.08661090309728	23.800523953830506
84-85	23.707458484338527	26.328453655030703	25.936560551065348	24.02752730956542
86-87	23.383522616001347	26.557830387927687	25.98621627565965	24.07243072041131
88-89	23.574286271158908	26.16977553871936	26.093226995053666	24.162711195068077
90-91	23.68178136432057	26.087795879158126	26.104157806852772	24.12626494966853
92-93	23.79308298920402	26.13166358962759	26.097988436884233	23.977264984284155
94-95	23.764990538390496	26.155828594803243	26.006644564470466	24.072536302335795
96-97	23.496769525326382	26.133876099122745	26.114662672251615	24.254691703299265
98-99	23.323310494176802	26.16158015691066	26.030821273325465	24.48428807558707
100-101	23.422505973130704	26.264253759033863	26.134025719748056	24.179214548087383
102-103	23.27735809352001	26.208468176664773	26.227564866170216	24.286608863645
104-105	23.31549131522619	26.25191545219504	26.116895192203405	24.31569804037537
106-107	23.31642170649138	26.17855868997352	26.17190713878311	24.333112464751995
108-109	23.27971107902011	26.167901134997045	26.164007571127645	24.3883802148552
110-111	23.171366227361165	26.622979467016165	26.01566945129109	24.18998485433158
112-113	23.114945277838032	26.585232339750185	26.141443300498178	24.158379081913598
114-115	23.12869423950665	26.69435306414028	25.982301254115008	24.194651442238055
116-117	23.04944281382206	26.548273053092885	26.086347924732067	24.315936208352984
118-119	23.075712608989203	26.315303294434884	26.225273402839782	24.383710693736134
120-121	23.04929436607673	26.44304924337678	26.23892981810032	24.268726572446173
122-123	23.057680760341405	26.533641065406815	26.101637331463273	24.307040842788506
124-125	22.915098502349	26.413085999058172	26.145532446632973	24.526283051959847
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	868.0
1	733.5
2	510.0
3	382.0
4	282.5
5	229.5
6	245.0
7	347.0
8	471.5
9	522.5
10	506.5
11	467.5
12	512.5
13	560.0
14	596.0
15	651.0
16	713.5
17	782.5
18	875.5
19	992.0
20	1131.0
21	1290.0
22	1487.0
23	1830.0
24	2245.5
25	2805.5
26	3795.0
27	5061.0
28	6420.5
29	8444.5
30	11288.5
31	14784.0
32	19470.0
33	24593.5
34	30035.5
35	35052.5
36	40392.5
37	46383.0
38	50793.5
39	55046.5
40	59381.0
41	63472.5
42	67504.0
43	72334.0
44	77468.5
45	81699.0
46	84792.0
47	87567.0
48	91452.0
49	93419.5
50	92737.5
51	91560.5
52	89301.0
53	86678.0
54	84871.5
55	81025.5
56	75046.5
57	68100.5
58	60949.0
59	53881.0
60	46181.0
61	38916.0
62	31524.5
63	24060.5
64	18176.5
65	14018.5
66	10808.5
67	8447.0
68	6658.0
69	5161.5
70	4130.5
71	3279.5
72	2497.5
73	1964.5
74	1488.5
75	1132.5
76	832.0
77	541.5
78	341.5
79	223.5
80	164.5
81	126.0
82	100.0
83	69.5
84	47.0
85	41.0
86	31.5
87	26.5
88	28.0
89	25.0
90	18.5
91	12.5
92	7.5
93	10.5
94	11.5
95	5.5
96	2.5
97	5.0
98	5.5
99	3.5
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013858395012460967
2	0.009872368353358483
3	0.009594273470165286
4	0.012375222302097252
5	0.00750856184621631
6	0.029987898237666375
7	0.012606968038091583
8	0.012097127418904056
9	0.008528243084591365
10-11	0.007578085567014611
12-13	0.00614126200384976
14-15	0.005515548516665068
16-17	0.0032907894511194943
18-19	0.005492373943065635
20-21	0.004542216425488879
22-23	0.004634914719886612
24-25	0.004913009603079808
26-27	0.0037079317759092893
28-29	0.005214279059872437
30-31	0.004519041851889446
32-33	0.007462212699017444
34-35	0.00727681611022198
36-37	0.0063730077398440905
38-39	0.006048563709452028
40-41	0.006280309445446358
42-43	0.00660475347583842
44-45	0.006627928049437855
46-47	0.00871363967338683
48-49	0.007415863551818579
50-51	0.004866660455880942
52-53	0.004333645263093981
54-55	0.005515548516665068
56-57	0.006998721227028783
58-59	0.006326658592645224
60-61	0.004125074100699084
62-63	0.004843485882281509
64-65	0.0023406319335427385
66-67	0.0030822182887245966
68-69	0.005747294252659399
70-71	0.0038238046439064543
72-73	0.003221265730321195
74-75	0.0028041234055314
76-77	0.0034993606135143916
78-79	0.0022711082127444397
80-81	0.002433330227940471
82-83	0.004403168983892281
84-85	0.008736814246986264
86-87	0.0063730077398440905
88-89	0.0038238046439064543
90-91	0.004148248674298517
92-93	0.007415863551818579
94-95	0.006233960298247492
96-97	0.008875861688582861
98-99	0.006071738283051461
100-101	0.007763482155810074
102-103	0.004403168983892281
104-105	0.0038469792175058874
106-107	0.006743800917435019
108-109	0.006048563709452028
110-111	0.007160943242224814
112-113	0.0043104706894945485
114-115	0.0018307913143552116
116-117	0.002062537050349542
118-119	0.15075060126431203
120-121	0.010266336104548844
122-123	0.0015063472839631487
124-125	0.001158728679971653
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2157537.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.3964513126706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.52343420882323	51.8542341055752
2	11.239376723516111	14.47551951921337
3	3.548811450824884	6.855925914326779
4	1.5459631609119768	3.98218165691402
5	0.8552444950817082	2.753735524397939
6	0.5303171384700845	2.049032507265816
7	0.34817774223180153	1.569498771805005
8	0.2468751181550593	1.2718305221265667
9	0.1835694431008757	1.063909863462564
>10	0.9161221349492018	10.299903937839701
>50	0.046924558364940035	2.05989875433548
>100	0.014680811929083338	1.4852461773980337
>500	2.8743636626726176E-4	0.1208695292367893
>1k	2.1557727470028539E-4	0.15821321610273414
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.634914719886611E-5
2	0.0	0.0	0.0	0.0	4.634914719886611E-5
3	0.0	0.0	0.0	0.0	4.634914719886611E-5
4	0.0	0.0	0.0	0.0	4.634914719886611E-5
5	0.0	0.0	0.0	4.634914719886611E-5	4.634914719886611E-5
6	0.0	0.0	0.0	4.634914719886611E-5	4.634914719886611E-5
7	0.0	0.0	0.0	4.634914719886611E-5	4.634914719886611E-5
8	0.0	0.0	0.0	4.634914719886611E-5	4.634914719886611E-5
9	0.0	0.0	0.0	4.634914719886611E-5	9.269829439773222E-5
10-11	0.0	0.0	0.0	4.634914719886611E-5	9.269829439773222E-5
12-13	0.0	0.0	0.0	1.3904744159659834E-4	1.1587286799716529E-4
14-15	0.0	0.0	0.0	1.3904744159659834E-4	2.3174573599433058E-4
16-17	0.0	0.0	0.0	1.3904744159659834E-4	3.244440303920628E-4
18-19	0.0	0.0	0.0	1.3904744159659834E-4	3.244440303920628E-4
20-21	0.0	0.0	0.0	1.622220151960314E-4	3.244440303920628E-4
22-23	0.0	0.0	0.0	3.476186039914959E-4	3.93967751190362E-4
24-25	0.0	0.0	0.0	5.561897663863934E-4	4.6349147198866117E-4
26-27	0.0	0.0	0.0	8.806337967784562E-4	4.6349147198866117E-4
28-29	0.0	0.0	0.0	0.00250285394873877	4.6349147198866117E-4
30-31	0.0	0.0	0.0	0.009988241221355647	4.6349147198866117E-4
32-33	0.0	0.0	0.0	0.02794853576091627	4.6349147198866117E-4
34-35	0.0	0.0	0.0	0.04683581324445421	4.6349147198866117E-4
36-37	0.0	0.0	0.0	0.07703228264451548	4.6349147198866117E-4
38-39	0.0	0.0	0.0	0.1266722192945011	4.6349147198866117E-4
40-41	0.0	0.0	0.0	0.18064580120758067	4.6349147198866117E-4
42-43	0.0	0.0	0.0	0.23556954063823704	4.866660455880942E-4
44-45	0.0	0.0	0.0	0.2964723200575471	5.330151927869603E-4
46-47	0.0	0.0	0.0	0.364860486749474	5.793643399858265E-4
48-49	0.0	4.634914719886611E-5	0.0	0.4336657957661908	6.025389135852595E-4
50-51	0.0	4.634914719886611E-5	0.0	0.509400302289138	6.025389135852595E-4
52-53	0.0	4.634914719886611E-5	0.0	0.584903063076091	6.257134871846925E-4
54-55	0.0	4.634914719886611E-5	0.0	0.6664775621460952	6.720626343835587E-4
56-57	0.0	4.634914719886611E-5	0.0	0.7608444258429867	6.952372079829917E-4
58-59	0.0	4.634914719886611E-5	0.0	0.8607963617773415	6.952372079829917E-4
60-61	0.0	4.634914719886611E-5	0.0	0.9638073414268213	6.952372079829917E-4
62-63	0.0	4.634914719886611E-5	0.0	1.0697614919234293	6.952372079829917E-4
64-65	0.0	4.634914719886611E-5	0.0	1.1825753162054695	6.952372079829917E-4
66-67	0.0	4.634914719886611E-5	0.0	1.309224360926371	6.952372079829917E-4
68-69	0.0	4.634914719886611E-5	0.0	1.451145449649299	6.952372079829917E-4
70-71	0.0	4.634914719886611E-5	0.0	1.6023363678120004	6.952372079829917E-4
72-73	0.0	4.634914719886611E-5	0.0	1.752947921634716	9.733320911761884E-4
74-75	0.0	4.634914719886611E-5	0.0	1.9126670828820085	9.733320911761884E-4
76-77	0.0	4.634914719886611E-5	0.0	2.0955376431551347	9.733320911761884E-4
78-79	0.0	4.634914719886611E-5	0.0	2.2984542095917706	9.733320911761884E-4
80-81	0.0	4.634914719886611E-5	2.3174573599433056E-5	2.5028307741651705	9.965066647756214E-4
82-83	0.0	4.634914719886611E-5	4.634914719886611E-5	2.7174273256959207	0.0010196812383750544
84-85	0.0	4.634914719886611E-5	4.634914719886611E-5	2.9436575131735863	0.0010196812383750544
86-87	0.0	4.634914719886611E-5	4.634914719886611E-5	3.1967933806001936	0.0010196812383750544
88-89	0.0	4.634914719886611E-5	4.634914719886611E-5	3.4752127078237827	0.0010196812383750544
90-91	0.0	4.634914719886611E-5	4.634914719886611E-5	3.769576141683781	0.0010196812383750544
92-93	0.0	4.634914719886611E-5	4.634914719886611E-5	4.075526862343496	0.0010196812383750544
94-95	0.0	4.634914719886611E-5	4.634914719886611E-5	4.384606150439135	0.0010660303855739206
96-97	0.0	4.634914719886611E-5	4.634914719886611E-5	4.728377775213126	0.0010660303855739206
98-99	0.0	4.634914719886611E-5	4.634914719886611E-5	5.10299475744796	0.0010660303855739206
100-101	0.0	4.634914719886611E-5	4.634914719886611E-5	5.506579956682087	0.0010660303855739206
102-103	0.0	4.634914719886611E-5	4.634914719886611E-5	5.921404824111939	0.0011355541063722198
104-105	0.0	4.634914719886611E-5	4.634914719886611E-5	6.3616521987803685	0.001158728679971653
106-107	0.0	4.634914719886611E-5	4.634914719886611E-5	6.832559534320849	0.001181903253571086
108-109	0.0	4.634914719886611E-5	4.634914719886611E-5	7.333338895230996	0.001251426974369385
110-111	0.0	4.634914719886611E-5	4.634914719886611E-5	7.865751549104372	0.0013209506951676843
112-113	0.0	4.634914719886611E-5	4.634914719886611E-5	8.41359846899497	0.0013441252687671173
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1935	0.0	57.211113	1
GTATCAA	3410	0.0	53.23459	1
TCAACGC	4485	0.0	39.41144	4
CAACGCA	4550	0.0	38.717617	5
ATCAACG	4790	0.0	36.77769	3
TATCAAC	4900	0.0	36.437912	2
AACGCAG	4850	0.0	36.197483	6
GTACATG	9565	0.0	35.903706	1
TACATGG	9900	0.0	34.44671	2
ACATGGG	10290	0.0	32.56277	3
CATGGGG	9170	0.0	29.984785	4
ATGGGGG	5745	0.0	28.592184	5
ACGCAGA	6115	0.0	28.310244	7
CGCAGAG	6485	0.0	26.786747	8
GTGGTAT	1210	0.0	25.086105	1
GCAGAGT	7435	0.0	22.80294	9
AGAGTAC	6680	0.0	21.684502	10-11
TGGTATC	1280	0.0	21.388294	2
TGGGGGG	4485	0.0	19.505316	6
GAGTACA	6865	0.0	19.246904	1
>>END_MODULE