FastQCFastQC Report
Mon 15 Aug 2016
SRR2921331_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921331_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2157537
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33060.15323028063945138No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26360.12217635201621108No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24640.11420429869800611No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28600.071.4400251
GTATCAA51350.049.79491
GTACATG96100.035.2485471
TATCAAC73200.034.7654952
TCAACGC69250.034.6830144
TACATGG97750.034.1430932
ATCAACG71600.033.9608653
CAACGCA71600.033.5446785
ACATGGG99700.033.2959673
AACGCAG74950.032.0431186
CATGGGG89600.031.1293074
ATGGGGG57450.028.3207155
ACGCAGA89100.026.2806197
GTGGTAT12500.025.808651
CGCAGAG91850.025.0975638
GAGTACT49900.023.32216812-13
AGAGTAC94650.022.93858510-11
GTACTTT52600.022.61934714-15
CAGAGTA97600.022.09268410-11
TGGTATC14100.021.5567192
Produced by FastQC (version 0.11.5)