##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921331_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2157537
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.873566478813572	31.0	30.0	34.0	16.0	34.0
2	29.694496548610754	33.0	31.0	34.0	25.0	34.0
3	29.93216802307446	33.0	31.0	34.0	26.0	34.0
4	32.00373898570453	37.0	33.0	37.0	19.0	37.0
5	31.66027650974236	37.0	33.0	37.0	19.0	37.0
6	31.801116736352608	37.0	33.0	37.0	17.0	37.0
7	32.034543555915846	37.0	35.0	37.0	17.0	37.0
8	32.29413493256431	37.0	35.0	37.0	17.0	37.0
9	34.01574944021817	39.0	35.0	39.0	17.0	39.0
10-11	34.228749263627925	39.0	37.0	39.0	17.0	39.0
12-13	34.26530043285469	39.0	37.0	39.0	17.0	39.0
14-15	35.63823378231753	40.0	38.0	41.0	19.0	41.0
16-17	35.6526138369817	40.0	38.0	41.0	19.0	41.0
18-19	35.68348746742234	40.5	38.0	41.0	19.0	41.0
20-21	35.675681344051114	41.0	38.0	41.0	19.0	41.0
22-23	35.62228179632609	40.5	38.0	41.0	18.5	41.0
24-25	35.5664410390181	41.0	37.5	41.0	18.0	41.0
26-27	35.46036081884111	40.0	37.0	41.0	17.5	41.0
28-29	35.38834768534676	40.0	37.0	41.0	16.5	41.0
30-31	35.28364936499351	40.0	37.0	41.0	16.0	41.0
32-33	35.152259266005636	40.0	36.0	41.0	14.5	41.0
34-35	35.05963234929459	40.0	36.0	41.0	9.5	41.0
36-37	34.970486253538176	40.0	36.0	41.0	9.0	41.0
38-39	34.86987175654461	40.0	35.5	41.0	8.5	41.0
40-41	34.741224600087975	40.0	35.0	41.0	4.5	41.0
42-43	34.594834063100656	40.0	35.0	41.0	2.0	41.0
44-45	34.43300091724963	40.0	35.0	41.0	2.0	41.0
46-47	34.20416752991953	40.0	35.0	41.0	2.0	41.0
48-49	33.98415021387814	40.0	35.0	41.0	2.0	41.0
50-51	33.81121158061252	40.0	35.0	41.0	2.0	41.0
52-53	33.633374074233714	39.0	35.0	41.0	2.0	41.0
54-55	33.448808525647536	39.0	34.5	41.0	2.0	41.0
56-57	33.22269003961462	39.0	34.0	41.0	2.0	41.0
58-59	33.173248709060374	39.0	34.0	41.0	2.0	41.0
60-61	32.9557520913894	38.0	34.0	41.0	2.0	41.0
62-63	32.69165140620995	37.0	34.0	40.5	2.0	41.0
64-65	32.409903051488804	37.0	34.0	40.0	2.0	41.0
66-67	32.10868434701236	36.5	33.5	39.5	2.0	41.0
68-69	31.785028020376938	36.0	33.0	39.0	2.0	41.0
70-71	31.46138397626553	35.0	33.0	39.0	2.0	41.0
72-73	31.12316335710581	35.0	33.0	37.5	2.0	40.0
74-75	30.788251603564618	35.0	33.0	37.0	2.0	39.0
76-77	30.475429621832674	35.0	32.0	37.0	2.0	39.0
78-79	30.2049920812482	35.0	32.0	36.0	2.0	38.5
80-81	29.95849178948032	35.0	32.0	36.0	2.0	37.0
82-83	29.739001926734048	35.0	32.0	35.0	2.0	37.0
84-85	29.55081303356559	35.0	32.0	35.0	2.0	36.5
86-87	29.393781659364357	35.0	32.0	35.0	2.0	36.0
88-89	29.255824581455613	35.0	31.0	35.0	2.0	36.0
90-91	29.14717175186335	35.0	31.0	35.0	2.0	36.0
92-93	29.06974248877308	35.0	31.0	35.0	2.0	35.0
94-95	28.992801513948542	35.0	31.0	35.0	2.0	35.0
96-97	28.92446294084412	35.0	31.0	35.0	2.0	35.0
98-99	28.868773281756003	35.0	31.0	35.0	2.0	35.0
100-101	28.812380274359143	35.0	31.0	35.0	2.0	35.0
102-103	28.15401080027828	34.0	29.5	35.0	2.0	35.0
104-105	28.68728578003529	35.0	31.0	35.0	2.0	35.0
106-107	28.748011273966565	35.0	31.0	35.0	2.0	35.0
108-109	28.813793459857237	35.0	31.0	35.0	2.0	35.0
110-111	28.915191952675666	35.0	32.0	35.0	2.0	35.0
112-113	28.877977758898226	35.0	32.0	35.0	2.0	35.0
114-115	28.82426998934433	35.0	32.0	35.0	2.0	35.0
116-117	28.763316457608838	35.0	32.0	35.0	2.0	35.0
118-119	28.686636428483034	35.0	31.0	35.0	2.0	35.0
120-121	28.594863726554863	35.0	31.0	35.0	2.0	35.0
122-123	28.48368672240615	35.0	31.0	35.0	2.0	35.0
124-125	27.968708531997365	34.5	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	161333.0
3	10448.0
4	7718.0
5	7230.0
6	7425.0
7	7806.0
8	8089.0
9	7850.0
10	8363.0
11	8304.0
12	9384.0
13	11444.0
14	13919.0
15	11657.0
16	7295.0
17	6290.0
18	5648.0
19	5089.0
20	4940.0
21	5079.0
22	5674.0
23	7092.0
24	9575.0
25	11648.0
26	13901.0
27	16333.0
28	19337.0
29	22724.0
30	27450.0
31	33311.0
32	43170.0
33	57812.0
34	86796.0
35	148583.0
36	324369.0
37	716671.0
38	297322.0
39	458.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.11975994996245	18.72641410724126	12.706236325123177	26.447589617673113
2	15.484554841933186	19.906726976177	38.5342174896653	26.07450069222451
3	18.810198851746225	23.413735198979207	28.967104619758548	28.80896132951602
4	13.285133889747017	16.894992833129667	32.034797797833065	37.78507547929025
5	13.993351224359035	37.84815533619961	32.86380224535468	15.294691194086676
6	33.39370821404425	35.082164555400674	16.146246323948795	15.377880906606272
7	29.274664364735013	32.22237624065323	19.074847857863755	19.428111536748
8	26.986895636498648	35.496365255336	18.12259632432593	19.39414278383942
9	26.846175453299008	16.734170573364494	18.408866944840238	38.01078702849626
10-11	26.26858317896839	26.214909834110895	25.60232454714274	21.914182439777974
12-13	26.026946215391177	23.59682470119984	26.571226840016553	23.805002243392426
14-15	23.698295788206643	24.6729951792252	24.02222997797952	27.606479054588632
16-17	23.47496242242891	27.698157667747992	24.71867226378968	24.108207646033417
18-19	23.63224825344826	26.62151332746553	26.72206780231347	23.02417061677274
20-21	24.411725036465192	25.48016557769345	26.768162029202742	23.339947356638614
22-23	24.21022211901812	25.141306962522542	26.58225559978809	24.066215318671244
24-25	23.882209204291748	25.638586962819176	26.4715738362772	24.007629996611875
26-27	23.58070800176312	25.708921793693456	26.684872611686384	24.02549759285704
28-29	23.567243574501852	25.952648784238697	26.640377430375473	23.839730210883985
30-31	24.07124420114232	25.685005633738843	26.540170574131523	23.703579590987314
32-33	23.620660966648543	25.873878408574218	26.605731442844316	23.899729181932916
34-35	23.851688290861293	25.907272969131007	26.79692167503964	23.44411706496806
36-37	23.96955417218801	25.82085498417872	26.735879848178733	23.47371099545454
38-39	23.729442415124282	26.09049114800812	26.702809731652344	23.47725670521525
40-41	23.68710246915812	26.074848310828504	26.597272723480526	23.640776496532855
42-43	23.54506550756719	26.072808948351756	26.45882782079751	23.92329772328354
44-45	23.664345037883475	26.069865777504624	26.545292154896995	23.720497029714902
46-47	23.66661614609622	26.10738541216211	26.10532287511176	24.120675566629913
48-49	23.643928238542376	26.381633316137798	25.999507772056745	23.974930673263078
50-51	23.62202826649091	26.413961846309007	26.081267667715547	23.88274221948453
52-53	23.789904877645203	26.240222068034058	26.06574070340393	23.90413235091681
54-55	23.419412969511065	26.08207877779153	26.266617907363816	24.23189034533359
56-57	23.524231565901303	26.20096434035662	25.892209496291375	24.382594597450705
58-59	23.59180862251725	26.258390933736013	26.081267667715547	24.068532776031187
60-61	23.931269776601745	26.008962998085316	26.245668092829927	23.814099132483012
62-63	23.55368644894618	26.04034137073895	26.345921298221075	24.060050882093794
64-65	23.421545030282214	25.813300073185303	26.335979406146915	24.429175490385564
66-67	23.441985004196916	26.17790563962518	26.068660699677455	24.311448656500446
68-69	23.391765703206943	26.070375618123816	26.15966725020243	24.37819142846681
70-71	23.355960986995818	25.947411330605224	26.297208344515067	24.39941933788389
72-73	23.438671040172196	26.201474180975808	26.02154679154981	24.338307987302187
74-75	23.288314406659076	26.19479990377917	26.14984123099627	24.367044458565484
76-77	23.233877333274005	25.835733060429554	26.195425617266356	24.734963989030085
78-79	23.17485169431625	25.901502500304748	26.25971188443118	24.663933920947823
80-81	23.342566083455345	26.12462729492009	26.028174719599246	24.50463190202532
82-83	23.23948558008507	26.146504092397954	26.16854311189101	24.445467215625964
84-85	23.269543002043534	26.025903611386504	26.033110903775924	24.67144248279404
86-87	22.97907289654824	26.297532788545457	26.08594893158263	24.63744538332367
88-89	23.0094084133899	26.026598848594485	26.097281298072755	24.86671143994286
90-91	23.06831104285422	25.920917639593878	26.18800478601627	24.822766531535635
92-93	23.19651490523195	25.896337063059583	26.19943815407519	24.707709877633278
94-95	23.142052587315483	25.945899847302627	26.076141041479968	24.835906523901922
96-97	22.932931886710293	25.898845925932452	26.098240557897096	25.069981629460163
98-99	22.67970901071662	25.879376779952505	26.126603188404925	25.31431102092595
100-101	22.837383553561306	25.980388748837214	26.19642212393113	24.985805573670348
102-103	22.703434518156584	25.871862220671073	26.195587839281554	25.229115421890796
104-105	22.674929792629282	25.948546884711593	26.10666700038053	25.2698563222786
106-107	22.680762379112647	25.78047962594358	26.179522886181942	25.359235108761823
108-109	22.60723544811997	25.655882964342798	26.260857030758082	25.47602455677915
110-111	22.434138837402568	26.08629957113015	26.14796728297299	25.331594308494292
112-113	22.437317035639	26.11189956635477	26.15085625930465	25.299927138701584
114-115	22.30750994132884	26.1049944299822	26.0641607650588	25.523334863630158
116-117	22.14277421054815	25.97325037409755	26.201660188952573	25.682315226401727
118-119	22.12578755982813	25.76241538663692	26.350424523918388	25.761372529616562
120-121	22.08339609786168	25.838283040599574	26.335818422412615	25.74250243912613
122-123	22.039621729378826	25.93097205426829	26.12554884048829	25.903857375864597
124-125	21.760128628242732	25.827037376673523	26.145989254869733	26.266844740214008
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	223.0
1	181.0
2	154.0
3	145.0
4	121.0
5	147.5
6	288.0
7	751.0
8	1105.0
9	1195.0
10	1093.0
11	883.5
12	861.0
13	963.0
14	1064.0
15	1108.5
16	1226.0
17	1375.5
18	1486.5
19	1561.5
20	1719.0
21	1884.0
22	2019.5
23	2354.0
24	2810.5
25	3466.0
26	4401.5
27	5560.5
28	6803.5
29	8644.0
30	11283.0
31	14237.5
32	18227.0
33	22653.0
34	27196.0
35	31915.0
36	37185.5
37	42748.0
38	47415.0
39	52099.0
40	56562.0
41	60747.5
42	64873.0
43	69947.0
44	75381.5
45	80319.0
46	84080.0
47	86809.0
48	90593.5
49	93395.0
50	94214.5
51	93366.0
52	91093.0
53	88811.5
54	86528.5
55	82509.0
56	76522.5
57	69623.5
58	62665.5
59	55373.5
60	47403.0
61	40015.0
62	32759.0
63	25298.5
64	18950.5
65	14570.0
66	11533.5
67	9030.0
68	7068.0
69	5623.5
70	4529.0
71	3521.0
72	2721.5
73	2216.5
74	1715.0
75	1311.5
76	1018.5
77	716.0
78	501.0
79	385.5
80	322.5
81	266.5
82	228.5
83	202.0
84	183.0
85	174.0
86	169.5
87	153.0
88	134.0
89	129.0
90	120.0
91	107.0
92	112.5
93	113.0
94	102.5
95	92.0
96	70.0
97	51.0
98	37.0
99	19.0
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33148910076629046
2	0.0
3	0.0
4	0.018029818260358918
5	0.17413374602613998
6	0.04685898781805364
7	0.02354536677702399
8	0.007601260140614042
9	0.006071738283051461
10-11	0.0019234896087529437
12-13	0.05582754780103424
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	6.952372079829917E-5
92-93	2.780948831931967E-4
94-95	6.952372079829917E-5
96-97	3.012694567926297E-4
98-99	2.317457359943306E-5
100-101	0.0
102-103	0.0
104-105	0.0
106-107	6.952372079829917E-5
108-109	3.012694567926297E-4
110-111	2.780948831931967E-4
112-113	6.025389135852594E-4
114-115	1.622220151960314E-4
116-117	5.330151927869603E-4
118-119	1.1587286799716529E-4
120-121	9.269829439773224E-5
122-123	0.001575871004761448
124-125	6.952372079829917E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2157537.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.37601234110629
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.41406823986311	54.7031721056993
2	10.116141120115811	13.4293821566636
3	3.222511464986344	6.416923823078704
4	1.4186596680060697	3.766598865256025
5	0.7657457725114433	2.5413575423184764
6	0.47403655747713314	1.8878793833542642
7	0.30866411131404003	1.434152500328613
8	0.22474581837148874	1.1934184971070376
9	0.17277902511078402	1.0321544432733942
>10	0.8230398839880488	9.492062422606358
>50	0.044876099856875115	2.0075694854632524
>100	0.014313863375686955	1.5880622412748175
>500	1.3945834108469344E-4	0.07025826623800911
>1k	2.78916682169367E-4	0.43700826733810705
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3306	0.15323028063945138	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2636	0.12217635201621108	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2464	0.11420429869800611	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.634914719886611E-5	0.0
5	0.0	0.0	0.0	4.634914719886611E-5	4.634914719886611E-5
6	0.0	0.0	0.0	4.634914719886611E-5	4.634914719886611E-5
7	0.0	0.0	0.0	4.634914719886611E-5	4.634914719886611E-5
8	0.0	0.0	0.0	4.634914719886611E-5	9.269829439773222E-5
9	0.0	0.0	0.0	4.634914719886611E-5	9.269829439773222E-5
10-11	0.0	0.0	0.0	4.634914719886611E-5	9.269829439773222E-5
12-13	0.0	0.0	0.0	9.269829439773222E-5	1.3904744159659834E-4
14-15	0.0	0.0	0.0	9.269829439773222E-5	1.622220151960314E-4
16-17	0.0	0.0	0.0	1.1587286799716529E-4	1.8539658879546445E-4
18-19	0.0	0.0	0.0	1.3904744159659834E-4	1.8539658879546445E-4
20-21	0.0	0.0	0.0	1.622220151960314E-4	1.8539658879546445E-4
22-23	0.0	0.0	0.0	3.244440303920628E-4	2.549203095937636E-4
24-25	0.0	0.0	0.0	5.098406191875272E-4	3.244440303920628E-4
26-27	0.0	0.0	0.0	8.574592231790231E-4	3.707931775909289E-4
28-29	0.0	0.0	0.0	0.0024796793751393374	3.707931775909289E-4
30-31	0.0	0.0	0.0	0.009617448043764718	3.939677511903619E-4
32-33	0.0	0.0	0.0	0.026349490182555384	4.6349147198866117E-4
34-35	0.0	0.0	0.0	0.04421708642771827	4.6349147198866117E-4
36-37	0.0	0.0	0.0	0.07195705102623964	4.6349147198866117E-4
38-39	0.0	0.0	0.0	0.11747191357552617	4.6349147198866117E-4
40-41	0.0	0.0	0.0	0.16861819750947493	4.6349147198866117E-4
42-43	0.0	0.0	0.0	0.22052924237220498	4.866660455880942E-4
44-45	0.0	0.0	0.0	0.27688980536602614	5.793643399858263E-4
46-47	0.0	0.0	0.0	0.340133216718879	6.720626343835587E-4
48-49	0.0	0.0	0.0	0.4049524990764932	7.415863551818578E-4
50-51	0.0	0.0	0.0	0.4753336790979714	7.415863551818578E-4
52-53	0.0	0.0	0.0	0.5451123202058643	8.574592231790231E-4
54-55	0.0	0.0	0.0	0.6214261910687975	0.0010196812383750544
56-57	0.0	0.0	0.0	0.7097908402034356	0.0010892049591733537
58-59	0.0	0.0	0.0	0.8032538955299492	0.001158728679971653
60-61	0.0	0.0	0.0	0.9005639300739686	0.001158728679971653
62-63	0.0	0.0	0.0	0.99942666104915	0.001205077827170519
64-65	0.0	0.0	0.0	1.1039439879825932	0.001205077827170519
66-67	0.0	0.0	0.0	1.221995265898105	0.001228252400769952
68-69	0.0	0.0	0.0	1.356361443627618	0.001251426974369385
70-71	0.0	0.0	0.0	1.496776185066583	0.001251426974369385
72-73	0.0	0.0	0.0	1.6374458468151416	0.0015526964311620148
74-75	0.0	0.0	0.0	1.7860412127347063	0.001575871004761448
76-77	0.0	0.0	0.0	1.9560730592337467	0.001575871004761448
78-79	0.0	0.0	0.0	2.144343295155541	0.001575871004761448
80-81	0.0	0.0	0.0	2.334374798670892	0.00166856929915918
82-83	0.0	0.0	0.0	2.5347189874379907	0.0018076167407557785
84-85	0.0	0.0	0.0	2.74648824098961	0.0018076167407557785
86-87	0.0	0.0	0.0	2.9819187341862503	0.0018307913143552116
88-89	0.0	0.0	0.0	3.2445098276414264	0.0018539658879546447
90-91	0.0	0.0	0.0	3.519012651926711	0.0018539658879546447
92-93	0.0	0.0	0.0	3.8051491121589107	0.0019003150351535106
94-95	0.0	0.0	0.0	4.0948544567254235	0.0019003150351535106
96-97	0.0	0.0	0.0	4.41503436557519	0.0019003150351535106
98-99	0.0	0.0	0.0	4.766893916535382	0.0019003150351535106
100-101	0.0	0.0	0.0	5.144871211942135	0.0019003150351535106
102-103	0.0	0.0	0.0	5.535779919417372	0.0019466641823523765
104-105	0.0	0.0	0.0	5.948287329487281	0.0019930133295512427
106-107	0.0	0.0	0.0	6.392497556241214	0.0019930133295512427
108-109	0.0	0.0	0.0	6.86602361859843	0.002016187903150676
110-111	0.0	0.0	0.0	7.370858529888479	0.002039362476750109
112-113	0.0	0.0	0.0	7.890316597119772	0.002039362476750109
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2860	0.0	71.440025	1
GTATCAA	5135	0.0	49.7949	1
GTACATG	9610	0.0	35.248547	1
TATCAAC	7320	0.0	34.765495	2
TCAACGC	6925	0.0	34.683014	4
TACATGG	9775	0.0	34.143093	2
ATCAACG	7160	0.0	33.960865	3
CAACGCA	7160	0.0	33.544678	5
ACATGGG	9970	0.0	33.295967	3
AACGCAG	7495	0.0	32.043118	6
CATGGGG	8960	0.0	31.129307	4
ATGGGGG	5745	0.0	28.320715	5
ACGCAGA	8910	0.0	26.280619	7
GTGGTAT	1250	0.0	25.80865	1
CGCAGAG	9185	0.0	25.097563	8
GAGTACT	4990	0.0	23.322168	12-13
AGAGTAC	9465	0.0	22.938585	10-11
GTACTTT	5260	0.0	22.619347	14-15
CAGAGTA	9760	0.0	22.092684	10-11
TGGTATC	1410	0.0	21.556719	2
>>END_MODULE