FastQCFastQC Report
Mon 15 Aug 2016
SRR2921330_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921330_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2293973
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20850.059.0778771
GTATCAA38650.058.1973041
TCAACGC49000.044.6882554
CAACGCA51150.042.808935
TATCAAC52550.042.461972
AACGCAG53650.040.923236
ATCAACG54000.040.4402583
GTACATG125550.039.528461
TACATGG130450.037.4034272
ACATGGG133300.036.246623
ATGGGGG78100.034.512735
CATGGGG125350.033.703554
ACGCAGA66650.032.6698277
CGCAGAG71400.029.9958178
TGGGGGG56650.027.9379776
GCAGAGT82850.025.633819
GTAAGTA15650.023.193012
AGAGTAC74350.022.5632110-11
CAGAGTA80000.020.93250310-11
GTATAAG10250.020.3191131
Produced by FastQC (version 0.11.5)