##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921330_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2293973 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.516971211082257 31.0 31.0 34.0 27.0 34.0 2 30.677601698014755 31.0 31.0 34.0 27.0 34.0 3 30.925013938699365 33.0 31.0 34.0 27.0 34.0 4 34.36665819519236 37.0 35.0 37.0 32.0 37.0 5 34.50185595035338 37.0 35.0 37.0 32.0 37.0 6 34.51669178320756 37.0 35.0 37.0 32.0 37.0 7 34.397246175085755 37.0 35.0 37.0 31.0 37.0 8 34.465015935235506 37.0 35.0 37.0 32.0 37.0 9 36.104766708239374 39.0 37.0 39.0 32.0 39.0 10-11 36.121338830055976 39.0 37.0 39.0 32.0 39.0 12-13 36.05924742793398 39.0 37.0 39.0 32.0 39.0 14-15 37.405255859593815 41.0 38.0 41.0 32.0 41.0 16-17 37.394959748872374 41.0 38.0 41.0 32.0 41.0 18-19 37.37539805394397 41.0 38.0 41.0 32.0 41.0 20-21 37.40399581860815 41.0 38.0 41.0 32.0 41.0 22-23 37.35107039184855 41.0 38.0 41.0 31.0 41.0 24-25 37.32749862356706 41.0 38.0 41.0 31.0 41.0 26-27 37.2883033060982 41.0 38.0 41.0 31.0 41.0 28-29 37.225945117924226 41.0 38.0 41.0 31.0 41.0 30-31 37.14655098381716 41.0 38.0 41.0 30.5 41.0 32-33 37.07106404478169 40.0 38.0 41.0 30.0 41.0 34-35 36.97794219897095 40.0 38.0 41.0 30.0 41.0 36-37 36.87978084310495 40.0 38.0 41.0 30.0 41.0 38-39 36.788239879022115 40.0 38.0 41.0 30.0 41.0 40-41 36.68848347386826 40.0 37.5 41.0 30.0 41.0 42-43 36.512953291080585 40.0 37.0 41.0 30.0 41.0 44-45 36.281634526648745 40.0 36.5 41.0 29.0 41.0 46-47 36.07475000795563 40.0 36.0 41.0 28.5 41.0 48-49 35.88479790302676 40.0 35.5 41.0 28.0 41.0 50-51 35.714874586579704 40.0 35.0 41.0 28.0 41.0 52-53 35.52436820311311 39.0 35.0 41.0 27.5 41.0 54-55 35.31639648766572 39.0 35.0 41.0 27.0 41.0 56-57 35.071038325211326 39.0 35.0 41.0 26.5 41.0 58-59 34.80616750938219 38.0 35.0 40.0 26.0 41.0 60-61 34.51805731802423 38.0 34.0 40.0 26.0 41.0 62-63 34.19385777426325 37.0 34.0 40.0 26.0 41.0 64-65 33.845235972698894 37.0 34.0 39.5 25.0 41.0 66-67 33.50300897177081 36.0 34.0 39.0 24.0 41.0 68-69 33.14373708844873 35.5 33.5 39.0 23.5 40.5 70-71 33.07408369671308 35.0 34.0 38.5 25.0 40.0 72-73 32.77403439360446 35.0 34.0 37.5 24.0 39.5 74-75 32.44242434414006 35.0 33.0 37.0 24.0 39.0 76-77 32.13898376310445 35.0 33.0 36.5 23.0 39.0 78-79 31.849325602350156 35.0 33.0 36.0 22.5 37.5 80-81 31.575534018926987 35.0 33.0 35.5 21.5 37.0 82-83 31.3525276888612 35.0 33.0 35.0 20.0 37.0 84-85 31.13837434006416 35.0 33.0 35.0 20.0 36.0 86-87 30.966933351002822 35.0 33.0 35.0 19.5 36.0 88-89 30.830913659402267 35.0 33.0 35.0 18.5 36.0 90-91 30.707644335831326 35.0 32.5 35.0 18.0 35.0 92-93 30.595500470145026 35.0 32.0 35.0 17.0 35.0 94-95 30.46706761587865 35.0 32.0 35.0 15.0 35.0 96-97 30.371687896936887 35.0 32.0 35.0 11.0 35.0 98-99 30.283816548843426 35.0 32.0 35.0 8.5 35.0 100-101 30.18564058949255 35.0 32.0 35.0 7.0 35.0 102-103 29.34269867169317 34.0 30.0 35.0 5.0 35.0 104-105 29.926177858239832 34.5 31.5 35.0 2.0 35.0 106-107 29.994145746266412 35.0 32.0 35.0 2.0 35.0 108-109 29.92231229399823 35.0 32.0 35.0 2.0 35.0 110-111 29.831760007637406 35.0 31.5 35.0 2.0 35.0 112-113 29.736100860820944 35.0 31.0 35.0 2.0 35.0 114-115 29.602425791410795 35.0 31.0 35.0 2.0 35.0 116-117 29.466763776208353 34.5 31.0 35.0 2.0 35.0 118-119 29.45929376675314 34.5 31.0 35.0 2.0 35.0 120-121 29.612186586328612 35.0 32.0 35.0 2.0 35.0 122-123 29.469029060063043 35.0 32.0 35.0 2.0 35.0 124-125 28.90608760434408 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 69693.0 3 10318.0 4 6421.0 5 5477.0 6 5781.0 7 6304.0 8 6781.0 9 7100.0 10 7851.0 11 8202.0 12 8049.0 13 7984.0 14 8218.0 15 7506.0 16 6509.0 17 6039.0 18 5862.0 19 5470.0 20 5688.0 21 6040.0 22 6915.0 23 8842.0 24 12095.0 25 15739.0 26 17777.0 27 19858.0 28 23818.0 29 29251.0 30 35655.0 31 44550.0 32 57911.0 33 78038.0 34 122852.0 35 201993.0 36 435234.0 37 770186.0 38 211879.0 39 87.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.77727773335414 17.463007515781047 12.598154670396507 25.1615600804683 2 16.285352922121664 19.399152371306883 39.68229401231987 24.633200694251585 3 19.252360162497418 24.747721801213586 28.341874126410715 27.658043909878284 4 13.75077333712354 15.37409461242894 35.1675574667416 35.70757458370593 5 14.736788412752318 37.531994369078234 32.78180875705118 14.949408461118258 6 33.315164115999266 34.36659492006125 17.79356513184747 14.524675832092013 7 30.090048141356483 31.59981723549503 20.439105674680924 17.87102894846756 8 27.473822486001676 35.034423043972815 19.24372631249436 18.24802815753115 9 28.69303715794393 15.841302725287909 18.174006528972313 37.29165358779584 10-11 26.82716930988572 25.264103925635865 26.691193716610996 21.21753304786742 12-13 26.73319619664764 22.55126113944935 26.944677619436757 23.770865044466248 14-15 24.320062602652985 23.681306285441504 24.031266450362004 27.96736466154351 16-17 24.695171194350856 26.626318541660716 24.555450953072963 24.123059310915465 18-19 24.805685336016474 26.273927765384634 25.64550309068105 23.274883807917846 20-21 25.79592135670538 25.321914980528376 25.072312892162568 23.80985077060367 22-23 25.64393922880746 24.799136822372866 25.124724807637815 24.432199141181858 24-25 24.97647502210814 25.037072139124007 25.161710381964554 24.8247424568033 26-27 24.654513384827187 25.228675083897667 25.293020603644678 24.82379092763047 28-29 24.834687541142834 25.813440181913457 24.933146978993285 24.418725297950424 30-31 25.537464892500484 25.306913384608176 24.929098381649172 24.22652334124217 32-33 25.13046619061393 25.279803957941287 25.062260399639285 24.52746945180549 34-35 25.05702889122321 25.499985068403063 25.20800150493059 24.234984535443143 36-37 24.987787788049623 25.580644085463906 25.137407819870038 24.294160306616437 38-39 25.118617917883075 25.61687093703523 24.982730730841574 24.281780414240124 40-41 25.11160471006753 25.27851042588532 25.088433654051556 24.521451209995597 42-43 24.817857625522603 25.360164965712066 24.984741543545454 24.837235865219874 44-45 24.586092029538666 25.330945454434485 25.36706444788075 24.7158980681461 46-47 24.61571159050508 25.160037972710658 25.213836279197515 25.010414157586748 48-49 24.560083006907796 25.470499632703152 25.16774675917752 24.801670601211534 50-51 24.68740756499523 25.415160550543707 25.289715963374103 24.60771592108696 52-53 24.75511186743181 25.365329564420747 25.196116491310367 24.68344207683708 54-55 24.3951169920029 25.00935116433985 25.62386870529315 24.9716631383641 56-57 24.6320636391815 25.22230510239092 25.155843538710972 24.98978771971661 58-59 24.665627493802347 25.41625586932492 25.322002265661325 24.596114371211407 60-61 25.102055318254777 25.20467736830679 25.319266178231047 24.374001135207386 62-63 24.961211381947237 25.283726915922315 25.329981171335614 24.425080530794826 64-65 24.682226410644454 24.999416929193842 25.401681292843442 24.916675367318263 66-67 24.722016300297593 25.276948338070206 25.20911580334581 24.79191955828639 68-69 24.47614928911502 25.184767317208635 25.42273166593354 24.916351727742803 70-71 24.596831763019797 25.025126833016365 25.586625179146527 24.79141622481731 72-73 24.5677828298901 25.571675814235334 25.081521620667257 24.779019735207314 74-75 24.514724733677046 25.411916978166023 25.216418393563565 24.85693989459337 76-77 24.499366244998892 24.927289828204984 25.4126055667993 25.160738359996813 78-79 24.35885894689761 25.01845657076806 25.53626500911004 25.086419473224296 80-81 24.685579144688084 25.260255460133397 25.160796024238195 24.89336937094032 82-83 24.751438683558497 25.33057407184993 25.256397485363586 24.66158975922799 84-85 24.708889489068056 25.437191649778452 25.055198681465953 24.79872017968754 86-87 24.33355181889777 25.623561215884155 25.20960118718967 24.8332857780284 88-89 24.325646300585518 25.19498766927232 25.347110965214576 25.132255064927588 90-91 24.67717547037094 24.96917306655675 25.43838329763287 24.91526816543944 92-93 24.73802642394761 25.19013833216242 25.328599102664345 24.743236141225626 94-95 24.71220446626931 25.067551189807723 25.274869475145344 24.945374868777616 96-97 24.354357038225448 25.132686070033483 25.392979213169642 25.11997767857143 98-99 24.2852574673072 25.06012362371777 25.30495586938008 25.349663039594944 100-101 24.28703026350322 25.218546978663227 25.428985092768023 25.06543766506553 102-103 24.157416398831224 25.090584507940246 25.558356520308084 25.19364257292045 104-105 24.34595019229579 25.149507686164206 25.36146463911081 25.143077482429195 106-107 24.37554153955814 24.997803869766752 25.517158697929098 25.109495892746015 108-109 24.360270483180685 24.954628107424252 25.631272012942595 25.05382939645247 110-111 24.12484177453173 25.472060657437584 25.446360939567736 24.95673662846295 112-113 24.12355902802754 25.489537027470398 25.464273834699814 24.92263010980225 114-115 24.27994374689941 25.609219684400227 25.28078597404092 24.830050594659443 116-117 24.054257001457128 25.395969087546415 25.4610330234895 25.08874088750696 118-119 24.05568015327521 25.209348547620912 25.631920749591913 25.10305054951197 120-121 23.979477060468426 25.32189982587284 25.757108739230404 24.94151437442833 122-123 24.001908516745075 25.512047457461613 25.564751814355112 24.9212922114382 124-125 23.95525683573699 25.40274645729155 25.4951857875783 25.146810919393165 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 238.0 1 224.5 2 206.0 3 181.0 4 140.0 5 128.0 6 134.5 7 193.0 8 254.5 9 254.5 10 257.0 11 264.0 12 287.5 13 329.0 14 347.0 15 369.5 16 401.5 17 445.0 18 504.5 19 549.5 20 579.0 21 644.0 22 762.5 23 919.0 24 1111.0 25 1358.5 26 1740.0 27 2377.5 28 3250.5 29 4508.5 30 6490.0 31 8925.5 32 11964.0 33 15606.5 34 19664.0 35 24178.0 36 29543.0 37 34821.0 38 40162.5 39 46133.0 40 51075.5 41 55493.0 42 60704.0 43 67833.0 44 75659.5 45 82422.5 46 87751.5 47 93564.0 48 101106.0 49 106760.0 50 108605.0 51 109527.5 52 109135.5 53 106979.0 54 105710.5 55 102953.0 56 96235.5 57 87375.5 58 79008.0 59 70541.5 60 59766.5 61 49510.0 62 40808.5 63 31459.5 64 23194.0 65 17312.5 66 13130.5 67 10063.5 68 7709.0 69 5801.0 70 4357.0 71 3241.5 72 2384.0 73 1826.0 74 1365.0 75 995.0 76 747.0 77 481.0 78 288.0 79 193.5 80 139.0 81 95.0 82 70.5 83 56.5 84 49.0 85 37.0 86 21.5 87 14.0 88 13.0 89 11.5 90 11.0 91 12.0 92 8.0 93 5.5 94 4.5 95 3.5 96 4.0 97 3.5 98 3.0 99 3.5 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017393404368752377 2 0.012205897802633248 3 0.01198793534187194 4 0.014908632316073468 5 0.009154423351974935 6 0.03125581687317157 7 0.013557265059353356 8 0.013339302598592048 9 0.008587720953995535 10-11 0.007803056095254827 12-13 0.006735040037524417 14-15 0.006124745147392754 16-17 0.004511822937759076 18-19 0.006190133885621147 20-21 0.005318284042575915 22-23 0.0053618765347281765 24-25 0.005863190194479185 26-27 0.0046643966602919916 28-29 0.005884986440555316 30-31 0.005470857765108831 32-33 0.007824852341330957 34-35 0.007824852341330957 36-37 0.007083779974742511 38-39 0.006909410006133464 40-41 0.007105576220818641 42-43 0.007105576220818641 44-45 0.007236353697275425 46-47 0.009612144519573682 48-49 0.008195388524625181 50-51 0.0057324127180224 52-53 0.005209302812195261 54-55 0.006146541393468886 56-57 0.007236353697275425 58-59 0.007040187482590248 60-61 0.0051875065661191305 62-63 0.005667023979794008 64-65 0.003639973094713844 66-67 0.003857935555475152 68-69 0.006190133885621147 70-71 0.004816970382824907 72-73 0.004490026691682945 74-75 0.004119490508388721 76-77 0.004599007922063599 78-79 0.003247640665343489 80-81 0.003182251927115097 82-83 0.005340080288652046 84-85 0.009306997074507852 86-87 0.006647855053219894 88-89 0.0047951741367487765 90-91 0.0051657103200429995 92-93 0.007955629817787743 94-95 0.006844021267905071 96-97 0.009285200828431721 98-99 0.006865817513981201 100-101 0.00815179603247292 102-103 0.005252895304347523 104-105 0.004838766628901038 106-107 0.007214557451199295 108-109 0.006626058807143764 110-111 0.007563297388417388 112-113 0.005318284042575915 114-115 0.0023975870683743883 116-117 0.0032912331574957506 118-119 0.16188071960742345 120-121 0.010767345561608615 122-123 0.001961662146851772 124-125 0.0015693297174814175 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2293973.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.63021558277828 #Duplication Level Percentage of deduplicated Percentage of total 1 78.68929485787916 45.34881026716381 2 11.066529221249478 12.755329295474457 3 3.8703856447350993 6.6915347728372225 4 1.87276367697435 4.317110977585133 5 1.1026113562797162 3.17718650832098 6 0.692418983295031 2.3942553168540472 7 0.5031914468503941 2.0299322092978827 8 0.3636202398111722 1.6764410248463515 9 0.27357671655928717 1.4189656638366313 >10 1.4734342705727064 14.892373760100122 >50 0.06820362081410733 2.650922003469671 >100 0.023212584985634336 2.2794040333946453 >500 6.059039950670781E-4 0.2284971351094746 >1k 1.5147599876674567E-4 0.13923703170948012 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.35924921522616E-5 0.0 4 0.0 0.0 0.0 4.35924921522616E-5 4.35924921522616E-5 5 0.0 0.0 0.0 4.35924921522616E-5 4.35924921522616E-5 6 0.0 0.0 0.0 4.35924921522616E-5 1.743699686090464E-4 7 0.0 0.0 0.0 8.71849843045232E-5 1.743699686090464E-4 8 0.0 0.0 0.0 8.71849843045232E-5 1.743699686090464E-4 9 0.0 0.0 0.0 8.71849843045232E-5 1.743699686090464E-4 10-11 0.0 0.0 0.0 1.307774764567848E-4 1.743699686090464E-4 12-13 0.0 0.0 0.0 1.307774764567848E-4 1.743699686090464E-4 14-15 0.0 2.17962460761308E-5 0.0 1.307774764567848E-4 2.397587068374388E-4 16-17 0.0 4.35924921522616E-5 0.0 1.743699686090464E-4 3.051474450658312E-4 18-19 0.0 4.35924921522616E-5 0.0 1.743699686090464E-4 3.051474450658312E-4 20-21 0.0 4.35924921522616E-5 0.0 1.961662146851772E-4 3.26943691141962E-4 22-23 0.0 4.35924921522616E-5 0.0 2.615549529135696E-4 3.705361832942236E-4 24-25 0.0 4.35924921522616E-5 0.0 4.35924921522616E-4 4.141286754464852E-4 26-27 0.0 4.35924921522616E-5 0.0 8.500535969691011E-4 5.449061519032701E-4 28-29 0.0 4.35924921522616E-5 0.0 0.0021360321154608184 5.667023979794008E-4 30-31 0.0 4.35924921522616E-5 0.0 0.00950316328919303 5.667023979794008E-4 32-33 0.0 4.35924921522616E-5 0.0 0.02811715743820873 5.667023979794008E-4 34-35 0.0 4.35924921522616E-5 0.0 0.050654475880927974 6.102948901316624E-4 36-37 0.0 6.538873822839239E-5 0.0 0.08136538660219628 6.102948901316624E-4 38-39 0.0 8.71849843045232E-5 0.0 0.14134865580370823 6.102948901316624E-4 40-41 0.0 8.71849843045232E-5 0.0 0.21876892186612484 6.102948901316624E-4 42-43 0.0 1.307774764567848E-4 0.0 0.28679500586972906 6.102948901316624E-4 44-45 0.0 1.307774764567848E-4 0.0 0.3593765053032446 6.53887382283924E-4 46-47 0.0 1.307774764567848E-4 0.0 0.4318272272603034 7.846648587407088E-4 48-49 0.0 1.307774764567848E-4 0.0 0.51724671563266 7.846648587407088E-4 50-51 0.0 1.307774764567848E-4 0.0 0.6111013512364792 8.282573508929704E-4 52-53 0.0 1.307774764567848E-4 0.0 0.7091408660869156 8.936460891213627E-4 54-55 0.0 1.307774764567848E-4 0.0 0.8153103807237487 9.154423351974936E-4 56-57 0.0 1.307774764567848E-4 0.0 0.9157692788886356 9.154423351974936E-4 58-59 0.0 1.307774764567848E-4 0.0 1.0407489538891697 9.372385812736245E-4 60-61 0.0 1.5257372253291558E-4 0.0 1.1709161354558226 9.590348273497552E-4 62-63 0.0 1.743699686090464E-4 0.0 1.3160355418307015 0.0011334047959588017 64-65 0.0 1.743699686090464E-4 0.0 1.4454834472768425 0.0011334047959588017 66-67 0.0 1.743699686090464E-4 0.0 1.5876385641853674 0.0011334047959588017 68-69 0.0 1.743699686090464E-4 0.0 1.7479499540753096 0.0011334047959588017 70-71 0.0 1.743699686090464E-4 0.0 1.9206852042286462 0.0011769972881110632 72-73 0.0 1.743699686090464E-4 0.0 2.089170186397137 0.0011987935341871941 74-75 0.0 1.743699686090464E-4 0.0 2.263104230084661 0.0012423860263394556 76-77 0.0 1.743699686090464E-4 0.0 2.4669427233886365 0.0012859785184917171 78-79 0.0 1.743699686090464E-4 0.0 2.6849705728881723 0.001307774764567848 80-81 0.0 1.961662146851772E-4 0.0 2.89983796670667 0.001394959748872371 82-83 0.0 2.17962460761308E-4 0.0 3.127281794511095 0.0015911259635575485 84-85 0.0 2.17962460761308E-4 0.0 3.372642136590099 0.0016129222096336793 86-87 0.0 2.17962460761308E-4 0.0 3.6242580012929535 0.001656514701785941 88-89 0.0 2.17962460761308E-4 0.0 3.909505473691277 0.0017872921782427257 90-91 0.0 2.17962460761308E-4 0.0 4.21092576067809 0.0017872921782427257 92-93 0.0 2.397587068374388E-4 0.0 4.509381758198549 0.0018308846703949872 94-95 0.0 2.615549529135696E-4 0.0 4.815836978028948 0.0018308846703949872 96-97 0.0 2.615549529135696E-4 0.0 5.148316915674247 0.0018962734086233795 98-99 0.0 2.615549529135696E-4 0.0 5.521250686036845 0.0019180696546995104 100-101 0.0 2.833511989897004E-4 0.0 5.917310273486217 0.0019180696546995104 102-103 0.0 3.051474450658312E-4 0.0 6.334076294707915 0.0020270508850801644 104-105 0.0 3.051474450658312E-4 0.0 6.774927167843737 0.0020488471311562954 106-107 0.0 3.26943691141962E-4 0.0 7.24134503762686 0.0020488471311562954 108-109 0.0 3.487399372180928E-4 0.0 7.72648588278938 0.0020706433772324263 110-111 0.0 3.487399372180928E-4 0.0 8.241552973814425 0.0021796246076130803 112-113 0.0 3.487399372180928E-4 0.0 8.763900011028902 0.002310402084069865 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2085 0.0 59.077877 1 GTATCAA 3865 0.0 58.197304 1 TCAACGC 4900 0.0 44.688255 4 CAACGCA 5115 0.0 42.80893 5 TATCAAC 5255 0.0 42.46197 2 AACGCAG 5365 0.0 40.92323 6 ATCAACG 5400 0.0 40.440258 3 GTACATG 12555 0.0 39.52846 1 TACATGG 13045 0.0 37.403427 2 ACATGGG 13330 0.0 36.24662 3 ATGGGGG 7810 0.0 34.51273 5 CATGGGG 12535 0.0 33.70355 4 ACGCAGA 6665 0.0 32.669827 7 CGCAGAG 7140 0.0 29.995817 8 TGGGGGG 5665 0.0 27.937977 6 GCAGAGT 8285 0.0 25.63381 9 GTAAGTA 1565 0.0 23.19301 2 AGAGTAC 7435 0.0 22.56321 10-11 CAGAGTA 8000 0.0 20.932503 10-11 GTATAAG 1025 0.0 20.319113 1 >>END_MODULE