FastQCFastQC Report
Mon 15 Aug 2016
SRR2921330_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921330_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2293973
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22750.060.9372561
GTATCAA41100.056.55671
GTACATG113800.042.1647531
TACATGG119050.039.7940032
ACATGGG121050.039.1857533
TCAACGC57450.039.001184
TATCAAC59600.038.4941142
CAACGCA59000.037.5725635
ATCAACG61600.036.3736653
CATGGGG114150.036.1251874
AACGCAG61600.035.7932326
ATGGGGG68600.034.920615
TGGGGGG49500.029.8556396
ACGCAGA78150.027.8234547
CGCAGAG83200.026.0607728
GTAAGTA11150.025.6534792
TAAGTAC15850.022.5580673
AGAGTAC83000.022.49830210-11
GCAGAGT92450.022.1646469
ATGGGGA41750.021.1244375
Produced by FastQC (version 0.11.5)