##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921330_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2293973
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.15535274390762	33.0	30.0	34.0	16.0	34.0
2	29.9308296130774	33.0	31.0	34.0	26.0	34.0
3	30.188120784333556	33.0	31.0	34.0	26.0	34.0
4	32.36874235224216	37.0	35.0	37.0	19.0	37.0
5	31.976791357178136	37.0	33.0	37.0	19.0	37.0
6	32.12692607977513	37.0	35.0	37.0	17.0	37.0
7	32.34717976192396	37.0	35.0	37.0	17.0	37.0
8	32.60020192042365	37.0	35.0	37.0	17.0	37.0
9	34.33924156910304	39.0	37.0	39.0	17.0	39.0
10-11	34.54308376776885	39.0	37.0	39.0	21.5	39.0
12-13	34.54990185150392	39.0	37.0	39.0	23.5	39.0
14-15	35.96886798580454	41.0	38.0	41.0	25.0	41.0
16-17	35.97446264624736	41.0	38.0	41.0	25.0	41.0
18-19	35.9983502421345	41.0	38.0	41.0	25.0	41.0
20-21	35.98788935179272	41.0	38.0	41.0	25.0	41.0
22-23	35.93436256660388	41.0	38.0	41.0	24.5	41.0
24-25	35.876726099217386	41.0	38.0	41.0	24.0	41.0
26-27	35.79183952906159	41.0	38.0	41.0	23.5	41.0
28-29	35.72336466035128	40.0	37.5	41.0	23.0	41.0
30-31	35.637325068778054	40.0	37.0	41.0	22.5	41.0
32-33	35.51884612416973	40.0	37.0	41.0	20.5	41.0
34-35	35.441979700720104	40.0	37.0	41.0	19.0	41.0
36-37	35.3669112496093	40.0	37.0	41.0	18.0	41.0
38-39	35.277668045787806	40.0	36.0	41.0	17.0	41.0
40-41	35.15111598959534	40.0	36.0	41.0	15.5	41.0
42-43	35.025365381371095	40.0	36.0	41.0	14.0	41.0
44-45	34.89164606558141	40.0	35.0	41.0	9.5	41.0
46-47	34.70924221863117	40.0	35.0	41.0	7.5	41.0
48-49	34.51994639867165	40.0	35.0	41.0	4.0	41.0
50-51	34.348907986275336	40.0	35.0	41.0	2.0	41.0
52-53	34.167568450020994	39.0	35.0	41.0	2.0	41.0
54-55	33.964006769042186	39.0	35.0	41.0	2.0	41.0
56-57	33.72002416767765	39.0	35.0	41.0	2.0	41.0
58-59	33.65475378306545	39.0	35.0	41.0	2.0	41.0
60-61	33.41318511595385	38.0	34.5	41.0	2.0	41.0
62-63	33.120196052874206	37.0	34.0	40.0	2.0	41.0
64-65	32.81868618331602	37.0	34.0	40.0	2.0	41.0
66-67	32.50176658574448	36.0	34.0	39.0	2.0	41.0
68-69	32.1636089439588	36.0	34.0	39.0	2.0	41.0
70-71	31.82966822190148	35.0	33.5	38.5	2.0	40.5
72-73	31.485387578668103	35.0	33.0	37.0	2.0	39.5
74-75	31.150321080500945	35.0	33.0	37.0	2.0	39.0
76-77	30.85158194974396	35.0	33.0	36.0	2.0	39.0
78-79	30.597552586713096	35.0	33.0	36.0	2.0	37.0
80-81	30.374907420444792	35.0	33.0	35.5	2.0	37.0
82-83	30.180019555591983	35.0	33.0	35.0	2.0	36.5
84-85	30.009475046131755	35.0	32.0	35.0	2.0	36.0
86-87	29.870367480349593	35.0	32.0	35.0	2.0	36.0
88-89	29.751082728523834	35.0	32.0	35.0	2.0	36.0
90-91	29.65411537101788	35.0	32.0	35.0	2.0	35.0
92-93	29.591142092779645	35.0	32.0	35.0	2.0	35.0
94-95	29.522348998876623	35.0	32.0	35.0	2.0	35.0
96-97	29.460705727573952	35.0	32.0	35.0	2.0	35.0
98-99	29.405728620171203	35.0	32.0	35.0	2.0	35.0
100-101	29.35201547707841	35.0	32.0	35.0	2.0	35.0
102-103	28.673617562194497	34.0	30.0	35.0	2.0	35.0
104-105	29.22451070696996	35.0	31.0	35.0	2.0	35.0
106-107	29.28720499325842	35.0	32.0	35.0	2.0	35.0
108-109	29.357255294635117	35.0	32.0	35.0	2.0	35.0
110-111	29.46782263784273	35.0	32.0	35.0	2.0	35.0
112-113	29.4364765845108	35.0	32.0	35.0	2.0	35.0
114-115	29.371985415695825	35.0	32.0	35.0	2.0	35.0
116-117	29.307485528382415	35.0	32.0	35.0	2.0	35.0
118-119	29.22819252885714	35.0	32.0	35.0	2.0	35.0
120-121	29.134828090827572	35.0	32.0	35.0	2.0	35.0
122-123	29.022333305579448	35.0	32.0	35.0	2.0	35.0
124-125	28.491976801819376	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	159789.0
3	9944.0
4	7340.0
5	6639.0
6	6794.0
7	7133.0
8	7535.0
9	7631.0
10	7943.0
11	7883.0
12	8168.0
13	9196.0
14	10385.0
15	9104.0
16	6608.0
17	5815.0
18	5331.0
19	5001.0
20	4752.0
21	4952.0
22	5667.0
23	7079.0
24	9231.0
25	12063.0
26	14279.0
27	16801.0
28	20219.0
29	23734.0
30	29287.0
31	36215.0
32	46262.0
33	62546.0
34	96572.0
35	163986.0
36	371108.0
37	804724.0
38	275993.0
39	264.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.62407599723182	17.680003919547783	12.198367613336611	27.497552469883786
2	15.390808871769632	19.64901069018685	38.21753786988775	26.742642568155773
3	18.287529975287416	24.176352555152132	28.584643324049587	28.95147414551087
4	13.66574725112776	16.376293554876725	31.81392866579521	38.144030528200304
5	14.314818308545316	37.39466402540641	31.74971264066632	16.540805025381953
6	33.22133381482659	34.47448107543961	17.066668062299296	15.237517047434501
7	29.709229265263872	31.438915297751567	19.93174391804038	18.92011151894418
8	27.69530577323408	35.32377848742793	18.25022070604135	18.73069503329664
9	28.267655295804023	16.0039427748346	17.801950984734606	37.92645094462677
10-11	26.888581942684663	25.278763655370373	25.814330774326173	22.018323627618795
12-13	26.63093128124202	22.707410152853498	26.221411528096638	24.440247037807847
14-15	24.25599603831431	23.768522994821648	23.759477552700055	28.216003414163985
16-17	24.29416126519362	26.657680800951013	24.302247672487862	24.745910261367506
18-19	24.603907718181514	25.96891942494528	25.63526249001187	23.791910366861334
20-21	25.4859364081443	25.020804516879668	25.226473894854035	24.266785180121996
22-23	25.22551486002669	24.525484824799594	25.137893950800645	25.111106364373075
24-25	24.71123243386038	25.011453927313003	25.062239180670392	25.215074458156224
26-27	24.428578714745118	25.031397492472667	25.36435694753164	25.17566684525058
28-29	24.645800103139837	25.410259841767974	25.076493925604183	24.86744612948801
30-31	25.21795156263827	25.186913708225863	25.06930116439906	24.525833564736814
32-33	24.809838651108798	25.225427675042383	25.207009847108054	24.75772382674077
34-35	24.888414118213248	25.373053649716017	25.304896788235954	24.433635443834778
36-37	24.969452561124303	25.36062978945262	25.251299819134747	24.418617830288326
38-39	24.842794575175905	25.486350536819746	25.172920518244986	24.497934369759363
40-41	24.798962324316808	25.47257530929963	25.11965049283492	24.608811873548643
42-43	24.628624661231846	25.477544853404986	24.978040282078297	24.915790203284867
44-45	24.31432279281404	25.4874839416157	25.424470994209607	24.773722271360647
46-47	24.366045284752698	25.357229575064743	25.02788829685441	25.24883684332815
48-49	24.428273567300053	25.64777353525957	24.925554921526974	24.998397975913406
50-51	24.423936114330903	25.63807420575569	25.13774137707811	24.8002483028353
52-53	24.582503804534753	25.501389946612274	25.05206033375284	24.86404591510013
54-55	24.163144030029997	25.15343467425292	25.52351313637955	25.159908159337537
56-57	24.36778898443879	25.31747322222188	25.0739219685672	25.240815824772135
58-59	24.388865954394408	25.44966745467362	25.211870409983028	24.949596180948948
60-61	24.839481545772337	25.239006736347815	25.251278022888673	24.67023369499118
62-63	24.613781417654003	25.336850084983563	25.296984750910323	24.752383746452118
64-65	24.39492531080357	24.95365028271911	25.34369410625147	25.30773030022585
66-67	24.300482176555697	25.37466657192565	25.100448000041848	25.224403251476808
68-69	24.10544936666648	25.264333974288277	25.282076118594247	25.348140540451
70-71	24.19710258141661	25.10339049326213	25.391711236357185	25.30779568896408
72-73	24.243702955527375	25.548317264414184	24.89591202686344	25.312067753195
74-75	24.10115550618948	25.47933214558323	25.086999716212876	25.332512632014414
76-77	24.097689903063372	24.932442535287034	25.255550087119595	25.714317474529995
78-79	23.980665857880627	25.00772676923399	25.444261985646737	25.56734538723865
80-81	24.21990145481224	25.315664133797565	24.981200737759337	25.483233673630856
82-83	24.260093732576628	25.391929198817948	25.015834972774307	25.332142095831117
84-85	24.184046630017004	25.437461556870982	24.852254145972946	25.526237667139064
86-87	23.85232956098437	25.59783833549915	24.99312328436298	25.556708819153496
88-89	23.823122591242356	25.288353437464174	25.124576444448127	25.76394752684535
90-91	24.094604550190283	25.015104818284463	25.20608377616098	25.684206855364284
92-93	24.08307272208917	25.252052558756322	25.16898681148718	25.49588790766733
94-95	24.10808068016538	25.07071251757051	25.032133136789188	25.78907366547492
96-97	23.7347022580108	25.127813577010293	25.090912419800464	26.046571745178436
98-99	23.57994701331424	25.1141851090194	25.141256052546403	26.16461182511996
100-101	23.64312919114567	25.167907381647474	25.21208837244379	25.976875054763067
102-103	23.495633993948488	25.069606311844122	25.264181400565743	26.170578293641643
104-105	23.591084897486784	25.187289734292804	25.125235808188634	26.09638956003178
106-107	23.581820254445297	24.96076126523859	25.257430293894362	26.199988186421752
108-109	23.586775825530072	24.903447763272812	25.31164919437542	26.1981272168217
110-111	23.35670551422612	25.407432933305696	25.185742686303396	26.050118866164784
112-113	23.347277215745542	25.434783651132083	25.19201536907623	26.025923764046144
114-115	23.403149737420165	25.409868224025693	25.064222751048508	26.12275928750563
116-117	23.131866670793443	25.179809072604108	25.27074348692066	26.417580769681788
118-119	23.174129537580633	25.02940865552803	25.375708243469013	26.420753563422323
120-121	23.1537080157343	25.070015098282695	25.47712600363693	26.299150882346083
122-123	23.070836793544718	25.25511838827168	25.245309915058623	26.42873490312498
124-125	22.80387467676153	25.16748242785875	25.114364953015993	26.914277942363725
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	61.0
1	59.0
2	84.0
3	96.0
4	83.5
5	110.5
6	198.0
7	438.0
8	644.0
9	716.0
10	706.0
11	665.5
12	686.0
13	713.0
14	757.0
15	797.5
16	863.0
17	938.0
18	989.0
19	1090.0
20	1166.5
21	1237.5
22	1371.5
23	1538.5
24	1752.5
25	2037.0
26	2406.0
27	2991.5
28	3834.5
29	4970.0
30	6560.0
31	8620.0
32	11465.5
33	14755.5
34	18169.0
35	22151.5
36	27333.5
37	32338.5
38	37234.0
39	43005.0
40	48401.0
41	52892.5
42	57906.0
43	65142.0
44	72119.5
45	78908.5
46	85028.0
47	90953.0
48	98528.5
49	104758.0
50	107965.0
51	109740.0
52	110255.5
53	109163.0
54	108110.5
55	104930.5
56	98225.5
57	89999.5
58	81762.5
59	72909.5
60	62173.5
61	51472.0
62	42059.0
63	32676.5
64	24202.0
65	18134.0
66	13876.5
67	10757.5
68	8267.0
69	6161.5
70	4694.0
71	3625.5
72	2702.5
73	2077.0
74	1657.5
75	1268.5
76	959.5
77	665.0
78	481.0
79	347.0
80	253.5
81	211.0
82	195.5
83	185.0
84	166.0
85	158.0
86	154.0
87	143.0
88	130.0
89	111.5
90	109.0
91	114.0
92	92.5
93	81.0
94	85.5
95	70.5
96	53.0
97	46.5
98	36.5
99	16.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3485219747573315
2	0.0
3	0.0
4	0.0212295436781514
5	0.18121398987695148
6	0.04821329632040133
7	0.024019463175896143
8	0.008631313446147797
9	0.006844021267905071
10-11	0.0021578283615369493
12-13	0.05612533364603681
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	1.307774764567848E-4
92-93	3.051474450658312E-4
94-95	6.53887382283924E-5
96-97	3.051474450658312E-4
98-99	2.1796246076130802E-5
100-101	0.0
102-103	0.0
104-105	2.1796246076130802E-5
106-107	1.0898123038065401E-4
108-109	3.2694369114196197E-4
110-111	2.833511989897004E-4
112-113	6.974798744361857E-4
114-115	1.7436996860904641E-4
116-117	5.231099058271392E-4
118-119	2.397587068374388E-4
120-121	1.525737225329156E-4
122-123	0.001656514701785941
124-125	4.3592492152261604E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2293973.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.87579185383573
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.4752280530397	49.59869024200642
2	9.64341740099455	11.741013409252036
3	3.3496018561085	6.117289961570486
4	1.6097328119582337	3.9197503840423655
5	0.9593213834196486	2.919972442899413
6	0.6275418970122973	2.29212659412491
7	0.42368953681260246	1.805470523755644
8	0.31948305590137205	1.555902720950343
9	0.24644677797252676	1.3502378483013606
>10	1.2612817775038485	13.441896060429537
>50	0.062153742984395764	2.560533597790879
>100	0.021170387281457294	2.139544837122201
>500	5.731193912637038E-4	0.2256016219887233
>1k	3.5819961953927455E-4	0.3319697557657283
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.35924921522616E-5
2	0.0	0.0	0.0	0.0	4.35924921522616E-5
3	0.0	0.0	0.0	0.0	4.35924921522616E-5
4	0.0	0.0	0.0	0.0	4.35924921522616E-5
5	0.0	0.0	0.0	0.0	4.35924921522616E-5
6	0.0	0.0	0.0	0.0	8.71849843045232E-5
7	0.0	0.0	0.0	0.0	8.71849843045232E-5
8	0.0	0.0	0.0	0.0	8.71849843045232E-5
9	0.0	0.0	0.0	0.0	1.307774764567848E-4
10-11	0.0	0.0	0.0	0.0	1.743699686090464E-4
12-13	0.0	2.17962460761308E-5	0.0	0.0	1.961662146851772E-4
14-15	0.0	8.71849843045232E-5	0.0	0.0	2.17962460761308E-4
16-17	0.0	1.307774764567848E-4	0.0	6.538873822839239E-5	2.17962460761308E-4
18-19	0.0	1.307774764567848E-4	0.0	1.08981230380654E-4	2.615549529135696E-4
20-21	0.0	1.307774764567848E-4	0.0	1.5257372253291558E-4	3.487399372180928E-4
22-23	0.0	1.307774764567848E-4	0.0	2.17962460761308E-4	3.923324293703544E-4
24-25	0.0	1.307774764567848E-4	0.0	3.923324293703544E-4	3.923324293703544E-4
26-27	0.0	1.307774764567848E-4	0.0	8.064611048168396E-4	3.923324293703544E-4
28-29	0.0	1.307774764567848E-4	0.0	0.001983458392927903	3.923324293703544E-4
30-31	0.0	1.307774764567848E-4	0.0	0.008871072152985236	3.923324293703544E-4
32-33	0.0	1.307774764567848E-4	0.0	0.026569623966803447	4.795174136748776E-4
34-35	0.0	1.5257372253291558E-4	0.0	0.04814790758217294	6.102948901316624E-4
36-37	0.0	1.743699686090464E-4	0.0	0.07787798723001535	6.102948901316624E-4
38-39	0.0	1.743699686090464E-4	0.0	0.13457002327403156	6.320911362077933E-4
40-41	0.0	1.961662146851772E-4	0.0	0.20793618756628784	6.53887382283924E-4
42-43	0.0	2.17962460761308E-4	0.0	0.27099272746453423	6.974798744361856E-4
44-45	0.0	2.17962460761308E-4	0.0	0.3381469616250933	8.500535969691012E-4
46-47	0.0	2.17962460761308E-4	0.0	0.40604226815224065	9.80831073425886E-4
48-49	0.0	2.397587068374388E-4	0.0	0.48518443765467156	0.0010462198116542784
50-51	0.0	2.615549529135696E-4	0.0	0.5724784031895754	0.0010462198116542784
52-53	0.0	2.615549529135696E-4	0.0	0.6646329315994566	0.0011116085498826707
54-55	0.0	2.615549529135696E-4	0.0	0.7642853686595266	0.0011987935341871941
56-57	0.0	2.615549529135696E-4	0.0	0.8582489854937263	0.0012641822724155864
58-59	0.0	2.615549529135696E-4	0.0	0.9751422532000158	0.0012641822724155864
60-61	0.0	2.615549529135696E-4	0.0	1.0985743947291446	0.001307774764567848
62-63	0.0	2.615549529135696E-4	0.0	1.236958761066499	0.0013513672567201096
64-65	0.0	2.615549529135696E-4	0.0	1.3587125916477656	0.0013513672567201096
66-67	0.0	2.615549529135696E-4	0.0	1.491756877696468	0.0013513672567201096
68-69	0.0	2.615549529135696E-4	0.0	1.6428048630040546	0.0013513672567201096
70-71	0.0	3.051474450658312E-4	0.0	1.8059715611299696	0.0014603484871007636
72-73	0.0	3.051474450658312E-4	0.0	1.964800806286735	0.0014821447331768943
74-75	0.0	3.051474450658312E-4	0.0	2.126790507124539	0.0014821447331768943
76-77	0.0	3.051474450658312E-4	0.0	2.317616641521064	0.0014821447331768943
78-79	0.0	3.051474450658312E-4	0.0	2.522893687066064	0.0014821447331768943
80-81	0.0	3.26943691141962E-4	0.0	2.7241602233330555	0.0014821447331768943
82-83	0.0	3.487399372180928E-4	0.0	2.9371095474968536	0.0015911259635575485
84-85	0.0	3.923324293703544E-4	0.0	3.1660137237883794	0.0016129222096336793
86-87	0.0	3.923324293703544E-4	0.0	3.4028299374055404	0.0017001071939382025
88-89	0.0	3.923324293703544E-4	0.0	3.6722533351525932	0.0017872921782427257
90-91	0.0	3.923324293703544E-4	0.0	3.955386571681532	0.0017872921782427257
92-93	0.0	3.923324293703544E-4	0.0	4.235969647419564	0.0017872921782427257
94-95	0.0	3.923324293703544E-4	0.0	4.522982615750054	0.0017872921782427257
96-97	0.0	3.923324293703544E-4	0.0	4.83458174965442	0.0018962734086233795
98-99	0.0	3.923324293703544E-4	0.0	5.185784662679116	0.001961662146851772
100-101	0.0	3.923324293703544E-4	0.0	5.558740229287791	0.0020052546390040334
102-103	0.0	3.923324293703544E-4	0.0	5.948435312882933	0.0021578283615369493
104-105	0.0	4.35924921522616E-4	0.0	6.361495972271687	0.00217962460761308
106-107	0.0	4.35924921522616E-4	0.0	6.803393065219163	0.00217962460761308
108-109	0.0	4.35924921522616E-4	0.0	7.26466702092832	0.002223217099765342
110-111	0.0	4.35924921522616E-4	0.0	7.747889796436139	0.0022886058379937343
112-113	0.0	4.35924921522616E-4	0.0	8.242119676212406	0.002310402084069865
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2275	0.0	60.937256	1
GTATCAA	4110	0.0	56.5567	1
GTACATG	11380	0.0	42.164753	1
TACATGG	11905	0.0	39.794003	2
ACATGGG	12105	0.0	39.185753	3
TCAACGC	5745	0.0	39.00118	4
TATCAAC	5960	0.0	38.494114	2
CAACGCA	5900	0.0	37.572563	5
ATCAACG	6160	0.0	36.373665	3
CATGGGG	11415	0.0	36.125187	4
AACGCAG	6160	0.0	35.793232	6
ATGGGGG	6860	0.0	34.92061	5
TGGGGGG	4950	0.0	29.855639	6
ACGCAGA	7815	0.0	27.823454	7
CGCAGAG	8320	0.0	26.060772	8
GTAAGTA	1115	0.0	25.653479	2
TAAGTAC	1585	0.0	22.558067	3
AGAGTAC	8300	0.0	22.498302	10-11
GCAGAGT	9245	0.0	22.164646	9
ATGGGGA	4175	0.0	21.124437	5
>>END_MODULE