FastQCFastQC Report
Mon 15 Aug 2016
SRR2921329_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921329_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2752592
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45950.059.316821
GGTATCA23400.055.441951
TCAACGC57400.046.96184
CAACGCA59250.045.495485
ATCAACG62950.043.010463
TATCAAC63100.042.908222
AACGCAG64500.042.252116
GTACATG137350.040.0784531
TACATGG144050.037.673852
ACATGGG147400.036.5754053
ACGCAGA77650.034.9352157
CATGGGG135950.034.00954
CGCAGAG82900.032.722798
ATGGGGG80600.031.6723775
GCAGAGT97850.027.357979
AGAGTAC85800.024.30126810-11
TACCGTA1653.220706E-421.6325327
CAGAGTA91150.021.56964510-11
TGGGGGG56950.020.5833056
ATGGGGA50350.020.5640285
Produced by FastQC (version 0.11.5)