FastQCFastQC Report
Mon 15 Aug 2016
SRR2921329_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921329_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2752592
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26000.065.973451
GTATCAA47100.053.1684151
GTACATG136450.040.6914181
TACATGG142000.039.0732352
ACATGGG148700.037.072243
TATCAAC69200.036.687792
TCAACGC65950.036.2366144
CATGGGG136000.034.9689064
CAACGCA68150.034.8919375
ATCAACG71600.033.7101023
AACGCAG72100.032.7324076
ATGGGGG79800.031.8891565
ACGCAGA90400.026.1655277
TGGGGGG52650.025.0156576
CGCAGAG97900.024.03588
ATGGGGA50150.023.4106435
AGAGTAC96750.022.53402710-11
GTAAGTA10250.022.0941752
GCAGAGT108650.020.5603249
CAGAGTA104350.020.3229129
Produced by FastQC (version 0.11.5)