FastQCFastQC Report
Mon 15 Aug 2016
SRR2921328_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921328_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2306564
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19200.058.2761231
GTATCAA36850.057.9817051
TCAACGC48250.044.2756774
CAACGCA49300.043.330815
TATCAAC51400.041.214972
GTACATG125400.040.72151
ATCAACG52750.040.3857963
AACGCAG53100.040.0040636
TACATGG129050.039.148742
ACATGGG133750.037.3281333
CATGGGG120950.035.3253674
ACGCAGA62950.033.455857
ATGGGGG73550.031.9568275
CGCAGAG68700.030.655028
TGGGGGG50950.026.9772826
GCAGAGT80700.025.6532259
ATGGGGA42800.023.078825
AGAGTAC70600.022.96113410-11
CAGAGTA75700.021.06058710-11
AGTACAT83400.020.9059372
Produced by FastQC (version 0.11.5)