##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921328_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2306564 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.83519642203728 31.0 31.0 34.0 28.0 34.0 2 30.834019780071134 33.0 31.0 34.0 28.0 34.0 3 31.020348449035016 33.0 31.0 34.0 28.0 34.0 4 34.4766067622663 37.0 35.0 37.0 32.0 37.0 5 34.49699032847127 37.0 35.0 37.0 32.0 37.0 6 34.52128750817233 37.0 35.0 37.0 32.0 37.0 7 34.516255781326684 37.0 35.0 37.0 32.0 37.0 8 34.49022398684797 37.0 35.0 37.0 32.0 37.0 9 36.144722192837484 39.0 37.0 39.0 32.0 39.0 10-11 36.14433612074063 39.0 37.0 39.0 32.0 39.0 12-13 36.06795909413309 39.0 37.0 39.0 32.0 39.0 14-15 37.411084843082605 41.0 38.0 41.0 32.0 41.0 16-17 37.39368840405035 41.0 38.0 41.0 32.0 41.0 18-19 37.384279170228965 41.0 38.0 41.0 32.0 41.0 20-21 37.412192984890076 41.0 38.0 41.0 32.0 41.0 22-23 37.35785631788235 41.0 38.0 41.0 31.5 41.0 24-25 37.32262360810279 41.0 38.0 41.0 31.0 41.0 26-27 37.28208387020694 41.0 38.0 41.0 31.0 41.0 28-29 37.21727860141752 41.0 38.0 41.0 31.0 41.0 30-31 37.1444226130296 40.0 38.0 41.0 30.0 41.0 32-33 37.07500897438788 40.0 38.0 41.0 30.0 41.0 34-35 36.966612892596956 40.0 38.0 41.0 30.0 41.0 36-37 36.866585102342704 40.0 38.0 41.0 30.0 41.0 38-39 36.77099681604326 40.0 38.0 41.0 30.0 41.0 40-41 36.67654095008853 40.0 37.0 41.0 30.0 41.0 42-43 36.48767257270988 40.0 37.0 41.0 29.5 41.0 44-45 36.23634332279529 40.0 36.5 41.0 29.0 41.0 46-47 36.023926715235305 40.0 36.0 41.0 28.0 41.0 48-49 35.82664907628836 40.0 35.5 41.0 28.0 41.0 50-51 35.65315833421488 39.5 35.0 41.0 28.0 41.0 52-53 35.45621994447151 39.0 35.0 41.0 27.0 41.0 54-55 35.250544749679605 39.0 35.0 41.0 27.0 41.0 56-57 35.00291537542422 38.5 35.0 40.5 26.0 41.0 58-59 34.731052119082754 38.0 35.0 40.0 26.0 41.0 60-61 34.44166626202438 38.0 34.0 40.0 26.0 41.0 62-63 34.11861409438455 37.0 34.0 40.0 25.5 41.0 64-65 33.76350190152972 36.5 34.0 39.5 24.5 41.0 66-67 33.4212393412886 36.0 34.0 39.0 24.0 41.0 68-69 33.06570010630531 35.5 33.0 39.0 23.0 40.0 70-71 33.00803684614864 35.0 34.0 38.5 24.0 40.0 72-73 32.710029073548355 35.0 33.5 37.0 24.0 39.5 74-75 32.37912236556193 35.0 33.0 37.0 23.0 39.0 76-77 32.07719252533205 35.0 33.0 36.5 23.0 39.0 78-79 31.786254359298074 35.0 33.0 36.0 22.0 37.5 80-81 31.505206879150112 35.0 33.0 35.5 20.5 37.0 82-83 31.282231709156996 35.0 33.0 35.0 20.0 37.0 84-85 31.06868831734129 35.0 33.0 35.0 20.0 36.0 86-87 30.899688237568952 35.0 32.5 35.0 19.0 36.0 88-89 30.755046467386123 35.0 32.0 35.0 18.0 36.0 90-91 30.63197986268753 35.0 32.0 35.0 17.5 35.0 92-93 30.513953438968095 35.0 32.0 35.0 16.5 35.0 94-95 30.386913608293547 35.0 32.0 35.0 13.0 35.0 96-97 30.28949099179559 35.0 32.0 35.0 10.0 35.0 98-99 30.204425717214 35.0 32.0 35.0 7.0 35.0 100-101 30.094030558007496 35.0 32.0 35.0 7.0 35.0 102-103 29.224976848680548 33.5 30.0 34.5 3.5 35.0 104-105 29.82563631444868 34.0 31.0 35.0 2.0 35.0 106-107 29.895881276218653 35.0 31.0 35.0 2.0 35.0 108-109 29.82092280985917 35.0 31.0 35.0 2.0 35.0 110-111 29.723039117926056 35.0 31.0 35.0 2.0 35.0 112-113 29.62229120891508 34.5 31.0 35.0 2.0 35.0 114-115 29.48598304664427 34.0 31.0 35.0 2.0 35.0 116-117 29.341071394507154 34.0 31.0 35.0 2.0 35.0 118-119 29.328809432558558 34.5 31.0 35.0 2.0 35.0 120-121 29.487719829148467 35.0 32.0 35.0 2.0 35.0 122-123 29.338537972499353 35.0 32.0 35.0 2.0 35.0 124-125 28.753303181702307 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 67459.0 3 10521.0 4 6572.0 5 5709.0 6 5999.0 7 6696.0 8 7003.0 9 7353.0 10 7993.0 11 8371.0 12 8352.0 13 8162.0 14 8076.0 15 7350.0 16 6693.0 17 6286.0 18 5966.0 19 5759.0 20 5902.0 21 6261.0 22 7292.0 23 9353.0 24 12562.0 25 15951.0 26 18348.0 27 20740.0 28 25345.0 29 30267.0 30 37401.0 31 46666.0 32 60732.0 33 81685.0 34 127572.0 35 208357.0 36 442483.0 37 757430.0 38 201825.0 39 72.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.664005397698695 17.501110066569307 12.390294202330447 25.444590333401557 2 16.03700690985278 19.863355233034092 39.39717431421793 24.7024635428952 3 19.153706701169725 24.78709118493983 28.66239801168034 27.396804102210105 4 13.717624786391125 15.399221490234371 35.39518021195897 35.487973511415525 5 14.506199871831448 37.87558458473996 32.772863301042605 14.845352242385982 6 33.154709603439706 34.207282747948845 17.81849770446244 14.819509944149004 7 29.64864910569839 31.74559331683587 20.687724854555935 17.91803272290981 8 27.364873214775848 35.042925163785114 19.518971062208642 18.073230559230396 9 28.40421205254746 15.75435301218297 18.404749702341462 37.4366852329281 10-11 26.60389654311224 25.348082166122083 26.84700774209661 21.201013548669067 12-13 26.494898619506586 22.773404532763493 27.014217701247254 23.717479146482667 14-15 24.17590183836282 23.84024887270205 24.188822407214708 27.795026881720432 16-17 24.53431990490967 26.74996536868423 24.681668295079188 24.034046431326914 18-19 24.61663231301395 26.432599347381764 25.79010510743574 23.160663232168545 20-21 25.556956476685283 25.40420886230993 25.34040865948468 23.698426001520108 22-23 25.53156065514634 24.94562992972647 25.23547356591352 24.28733584921367 24-25 24.882490690088897 25.201362461970167 25.247538060182627 24.668608787758306 26-27 24.484844385652515 25.486800214529843 25.408866221911786 24.619489177905855 28-29 24.665394414998723 25.81633920602564 25.214277863493017 24.303988515482615 30-31 25.260040508675896 25.509995037763076 25.040826267727965 24.189138185833063 32-33 24.92822016356524 25.522772266924736 25.072495250086934 24.47651231942309 34-35 24.826376957818624 25.68961977556424 25.319014386946716 24.164988879670425 36-37 24.83446457965829 25.709580751996796 25.268933408400486 24.187021259944427 38-39 24.99925207305837 25.766863584629558 25.13123407945369 24.102650262858383 40-41 24.919613938786924 25.460550562138774 25.192466950229147 24.427368548845156 42-43 24.612693414823337 25.511353954709055 25.151525531168584 24.72442709929903 44-45 24.535532050180034 25.487805433243633 25.419364713161407 24.557297803414926 46-47 24.542609592998364 25.33606062478388 25.385099839896284 24.736229942321472 48-49 24.40577476858933 25.58005778410718 25.400249355744275 24.613918091559214 50-51 24.518504740486186 25.557197357450477 25.429899900668097 24.494398001395243 52-53 24.547350831721168 25.502748412909693 25.359149366421235 24.590751388947904 54-55 24.23222863168328 25.21581798816727 25.700231031094134 24.851722349055315 56-57 24.396692638215033 25.4044234012756 25.359114105715907 24.839769854793463 58-59 24.531408933992722 25.576313897697776 25.401711425080492 24.490565743229006 60-61 24.931474064201495 25.427436215165798 25.423794216679834 24.21729550395287 62-63 24.701148752501176 25.411992204351776 25.45857965717358 24.428279385973468 64-65 24.5144347737866 25.14195446141199 25.559715607652674 24.783895157148734 66-67 24.576677195089434 25.481312230240782 25.31556002610066 24.626450548569125 68-69 24.37177988004667 25.374252567966398 25.485248203149762 24.768719348837166 70-71 24.393895938379266 25.19023435120445 25.66503709301577 24.750832617400516 72-73 24.41298666669557 25.68490218160652 25.299958702634033 24.602152449063873 74-75 24.256520404500073 25.60653766044123 25.46194327911441 24.67499865594428 76-77 24.254704771649756 25.121345279619845 25.55480644371075 25.069143505019653 78-79 24.228159345006887 25.273914606192065 25.609709568790706 24.888216480010335 80-81 24.520901863411126 25.496505697670337 25.27289549523536 24.70969694368318 82-83 24.45718376159074 25.430466820772708 25.53964032144182 24.572709096194732 84-85 24.38212728890022 25.522276717394654 25.292581841942607 24.803014151762515 86-87 24.09907190276734 25.721632398400008 25.43501509179623 24.74428060703642 88-89 24.188589954399273 25.330700118039438 25.561923013138255 24.918786914423034 90-91 24.472072122069545 25.11544933431552 25.62964382509193 24.782834718523013 92-93 24.574008646975713 25.28474036201271 25.50096895296497 24.640282038046607 94-95 24.493183149961595 25.24251744237345 25.52881018548327 24.735489222181684 96-97 24.1418654912327 25.158807809218604 25.693662928324727 25.00566377122397 98-99 24.032859200498095 25.22422525977872 25.529681501708733 25.213234038014456 100-101 24.067714816794854 25.327671570954784 25.6794396684977 24.925173943752657 102-103 23.922593389402962 25.278982387407662 25.72098812964519 25.077436093544186 104-105 24.063611770319348 25.282899513251767 25.592837584169637 25.06065113225925 106-107 24.103299856527975 25.064917946923586 25.818828957848535 25.012953238699897 108-109 24.07280304649653 25.07677592702352 25.815658863842017 25.034762162637925 110-111 23.888358472470088 25.580654224571663 25.693819362425014 24.837167940533238 112-113 23.856648956695693 25.675830355416313 25.647062853198594 24.820457834689403 114-115 24.00660744247753 25.717583861191677 25.54474547906126 24.73106321726953 116-117 23.78007869599893 25.485108090478505 25.766186907658263 24.968626305864305 118-119 23.817005418862642 25.348886643785722 25.808872239516468 25.025235697835168 120-121 23.789628821528733 25.396743494707486 25.952487906911404 24.861139776852383 122-123 23.762947374171393 25.547078788840498 25.78395278908459 24.906021047903522 124-125 23.63319986325658 25.50698360444646 25.745655935949973 25.11416059634699 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 404.0 1 360.5 2 278.0 3 224.0 4 180.5 5 158.5 6 168.5 7 177.5 8 192.5 9 199.5 10 222.5 11 262.5 12 273.5 13 296.5 14 342.5 15 370.0 16 405.0 17 454.5 18 490.0 19 541.0 20 602.0 21 675.0 22 787.0 23 939.0 24 1131.5 25 1397.0 26 1763.0 27 2344.5 28 3233.5 29 4593.0 30 6782.0 31 9581.0 32 13063.0 33 16960.0 34 21057.0 35 25823.0 36 31475.5 37 37354.5 38 42750.0 39 48492.0 40 53987.5 41 58643.5 42 63485.5 43 70364.0 44 77633.5 45 83900.0 46 89903.0 47 95908.0 48 102204.0 49 107193.5 50 108862.0 51 108995.0 52 107876.5 53 105933.5 54 105044.5 55 102085.0 56 95189.0 57 86150.5 58 78123.5 59 69120.5 60 58210.0 61 48517.5 62 39351.5 63 29925.0 64 21866.0 65 16116.5 66 12052.5 67 9070.0 68 6801.0 69 5026.0 70 3692.0 71 2717.5 72 1933.5 73 1277.5 74 920.5 75 634.5 76 407.0 77 265.5 78 172.0 79 103.5 80 62.5 81 46.0 82 35.0 83 31.5 84 28.5 85 18.0 86 10.5 87 11.0 88 10.5 89 10.5 90 8.0 91 3.0 92 3.5 93 4.5 94 3.0 95 3.0 96 2.5 97 2.5 98 3.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017168394200204287 2 0.012659522996110232 3 0.01278958658853602 4 0.015737694683520594 5 0.009798123962742851 6 0.034076661215557 7 0.015217440313817436 8 0.01478389500573147 9 0.009971542085977237 10-11 0.009191160531422497 12-13 0.0077387837493345085 14-15 0.007110143052609856 16-17 0.0054409936164788834 18-19 0.007023433990992663 20-21 0.006286406967246519 22-23 0.006416470559672309 24-25 0.006546534152098099 26-27 0.005419316351074585 28-29 0.006698275009928187 30-31 0.006264729701842222 32-33 0.008931033346570917 34-35 0.008779292488740828 36-37 0.007998910934186088 38-39 0.008194006322824772 40-41 0.008410778976867756 42-43 0.00810729726120758 44-45 0.008172329057420474 46-47 0.010686891844319082 48-49 0.009559674043295567 50-51 0.0067199522753324855 52-53 0.006156343374820729 54-55 0.007131820318014155 56-57 0.008410778976867756 58-59 0.007890524607164596 60-61 0.005982925251586342 62-63 0.006459825090480906 64-65 0.00444383940788116 66-67 0.004725643858137038 68-69 0.007240206645035646 70-71 0.005744475332139061 72-73 0.0052675754932444965 74-75 0.004855707450562828 76-77 0.0055927344743089726 78-79 0.004205389488433878 80-81 0.004118680426816685 82-83 0.006221375171033624 84-85 0.010556828251893292 86-87 0.0077387837493345085 88-89 0.005701120801330464 90-91 0.0061129888440121325 92-93 0.00903941967359241 94-95 0.007955556403377492 96-97 0.010340055597850308 98-99 0.00771710648393021 100-101 0.009321224123848287 102-103 0.006329761498055116 104-105 0.00587453892456485 106-107 0.008280715384441965 108-109 0.007673751953121612 110-111 0.008670906161719337 112-113 0.006156343374820729 114-115 0.0032082352798361545 116-117 0.00405364863060379 118-119 0.16364167653704817 120-121 0.012486104872875846 122-123 0.0027530127063458893 124-125 0.002362821929068519 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2306564.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.34647435920465 #Duplication Level Percentage of deduplicated Percentage of total 1 78.6914096783849 45.126749074111686 2 11.011238693915857 12.629114348474557 3 3.8073051142518075 6.550065753363301 4 1.8569546106067616 4.2595919985347 5 1.1021813632786848 3.160310764422716 6 0.7080001190369954 2.436078640360134 7 0.4937212476832301 1.9819221009602614 8 0.38418115142710796 1.7625147639683494 9 0.29770937340069653 1.5365324653396133 >10 1.555011849887678 15.756726139007249 >50 0.0716084340543524 2.7680121403692546 >100 0.020375954744367493 1.8579201970228247 >500 1.5120466382452738E-4 0.05202632626203163 >1k 1.5120466382448605E-4 0.12243528780328303 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.335453080859668E-5 2 0.0 0.0 0.0 0.0 4.335453080859668E-5 3 0.0 0.0 0.0 0.0 4.335453080859668E-5 4 0.0 0.0 0.0 0.0 8.670906161719336E-5 5 0.0 0.0 0.0 0.0 8.670906161719336E-5 6 0.0 0.0 0.0 0.0 1.3006359242579004E-4 7 0.0 0.0 0.0 0.0 1.3006359242579004E-4 8 0.0 0.0 0.0 0.0 1.3006359242579004E-4 9 0.0 0.0 0.0 0.0 2.601271848515801E-4 10-11 0.0 0.0 0.0 0.0 2.601271848515801E-4 12-13 0.0 4.335453080859668E-5 0.0 0.0 2.601271848515801E-4 14-15 0.0 4.335453080859668E-5 0.0 2.167726540429834E-5 3.4683624646877345E-4 16-17 0.0 4.335453080859668E-5 0.0 4.335453080859668E-5 4.335453080859668E-4 18-19 0.0 4.335453080859668E-5 0.0 4.335453080859668E-5 4.335453080859668E-4 20-21 0.0 4.335453080859668E-5 0.0 4.335453080859668E-5 5.419316351074585E-4 22-23 0.0 4.335453080859668E-5 0.0 6.503179621289502E-5 7.153497583418453E-4 24-25 0.0 4.335453080859668E-5 0.0 2.3844991944728175E-4 8.670906161719336E-4 26-27 0.0 4.335453080859668E-5 0.0 7.587042891504419E-4 9.104451469805304E-4 28-29 0.0 4.335453080859668E-5 0.0 0.002579594583111503 9.104451469805304E-4 30-31 0.0 4.335453080859668E-5 0.0 0.010990373559979259 9.754769431934253E-4 32-33 0.0 4.335453080859668E-5 0.0 0.030239785238996186 0.0011705723318321105 34-35 0.0 4.335453080859668E-5 0.0 0.054778449676661914 0.0011705723318321105 36-37 0.0 4.335453080859668E-5 0.0 0.09130464188290462 0.001213926862640707 38-39 0.0 4.335453080859668E-5 0.0 0.15197930774953566 0.001213926862640707 40-41 0.0 4.335453080859668E-5 0.0 0.22457646958853084 0.0012356041280450055 42-43 0.0 4.335453080859668E-5 0.0 0.29509261394871333 0.001278958658853602 44-45 0.0 4.335453080859668E-5 0.0 0.36994421138975553 0.001322313189662199 46-47 0.0 4.335453080859668E-5 0.0 0.4516891792293645 0.0013873449858750938 48-49 0.0 4.335453080859668E-5 0.0 0.5428637575198434 0.0014306995166836905 50-51 0.0 4.335453080859668E-5 0.0 0.6430560782185103 0.0014740540474922873 52-53 0.0 4.335453080859668E-5 0.0 0.7446790984338609 0.0015390858437051823 54-55 0.0 4.335453080859668E-5 0.0 0.8508109898533056 0.001647472170726674 56-57 0.0 4.335453080859668E-5 0.0 0.9618852977849304 0.0017341812323438672 58-59 0.0 4.335453080859668E-5 0.0 1.089759486404886 0.0018208902939610607 60-61 0.0 4.335453080859668E-5 0.0 1.222706155129448 0.0018642448247696574 62-63 0.0 4.335453080859668E-5 0.0 1.3688109239544188 0.0018642448247696574 64-65 0.0 4.335453080859668E-5 0.0 1.5111655258644459 0.0019292766209825524 66-67 0.0 4.335453080859668E-5 0.0 1.6674802866948415 0.0019943084171954477 68-69 0.0 4.335453080859668E-5 0.0 1.8424808503037418 0.002037662948004044 70-71 0.0 4.335453080859668E-5 0.0 2.0315066046292234 0.002037662948004044 72-73 0.0 4.335453080859668E-5 0.0 2.2201421681774276 0.002146049275025536 74-75 0.0 4.335453080859668E-5 0.0 2.4108587492044444 0.002167726540429834 76-77 0.0 4.335453080859668E-5 0.0 2.625290258583764 0.002232758336642729 78-79 0.0 4.335453080859668E-5 0.0 2.8664064816757744 0.002276112867451326 80-81 0.0 4.335453080859668E-5 0.0 3.1158250974176305 0.0023411446636642207 82-83 0.0 4.335453080859668E-5 0.0 3.372245469885076 0.002557917317707204 84-85 0.0 4.335453080859668E-5 0.0 3.643124578377188 0.002557917317707204 86-87 0.0 4.335453080859668E-5 0.0 3.933643289325594 0.002579594583111503 88-89 0.0 4.335453080859668E-5 0.0 4.258433756878196 0.002601271848515801 90-91 0.0 4.335453080859668E-5 0.0 4.604381235465393 0.002601271848515801 92-93 0.0 4.335453080859668E-5 0.0 4.957308793512775 0.0026879809101329945 94-95 0.0 4.335453080859668E-5 0.0 5.320836534342858 0.002731335440941591 96-97 0.0 6.503179621289502E-5 0.0 5.7130866518336365 0.002731335440941591 98-99 0.0 8.670906161719336E-5 0.0 6.137657572042224 0.002731335440941591 100-101 0.0 8.670906161719336E-5 0.0 6.603198523864935 0.002731335440941591 102-103 0.0 8.670906161719336E-5 0.0 7.086731605973213 0.002818044502558784 104-105 0.0 1.083863270214917E-4 0.0 7.592137048874429 0.002861399033367381 106-107 0.0 1.3006359242579004E-4 0.0 8.126568350152002 0.002861399033367381 108-109 2.167726540429834E-5 1.3006359242579004E-4 0.0 8.679577067881056 0.0028830762987716793 110-111 4.335453080859668E-5 1.3006359242579004E-4 0.0 9.277999656632115 0.002991462625793171 112-113 4.335453080859668E-5 1.3006359242579004E-4 0.0 9.888301386824732 0.003034817156601768 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1920 0.0 58.276123 1 GTATCAA 3685 0.0 57.981705 1 TCAACGC 4825 0.0 44.275677 4 CAACGCA 4930 0.0 43.33081 5 TATCAAC 5140 0.0 41.21497 2 GTACATG 12540 0.0 40.7215 1 ATCAACG 5275 0.0 40.385796 3 AACGCAG 5310 0.0 40.004063 6 TACATGG 12905 0.0 39.14874 2 ACATGGG 13375 0.0 37.328133 3 CATGGGG 12095 0.0 35.325367 4 ACGCAGA 6295 0.0 33.45585 7 ATGGGGG 7355 0.0 31.956827 5 CGCAGAG 6870 0.0 30.65502 8 TGGGGGG 5095 0.0 26.977282 6 GCAGAGT 8070 0.0 25.653225 9 ATGGGGA 4280 0.0 23.07882 5 AGAGTAC 7060 0.0 22.961134 10-11 CAGAGTA 7570 0.0 21.060587 10-11 AGTACAT 8340 0.0 20.905937 2 >>END_MODULE