FastQCFastQC Report
Mon 15 Aug 2016
SRR2921328_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921328_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2306564
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23900.061.7725871
GTATCAA44500.060.3091281
TCAACGC62650.040.9078564
TATCAAC66300.040.363812
GTACATG119650.039.8145981
CAACGCA65900.038.4381835
TACATGG123950.038.1316832
ATCAACG67600.037.824223
AACGCAG67750.036.8551336
ACATGGG128600.036.7065473
CATGGGG118550.034.639934
ATGGGGG74200.032.3713265
ACGCAGA82150.030.312427
CGCAGAG88300.027.8572038
TGGGGGG50450.027.0500226
GTAAGTA12400.025.4749582
AGAGTAC86900.024.50083510-11
GCAGAGT99600.023.7399259
GTGGTAT12250.023.4208071
TAAGTAC16650.022.5520293
Produced by FastQC (version 0.11.5)