##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921328_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2306564
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.08079073461651	31.0	30.0	34.0	16.0	34.0
2	29.8685685721272	33.0	31.0	34.0	26.0	34.0
3	30.135530165215446	33.0	31.0	34.0	26.0	34.0
4	32.28604539045958	37.0	35.0	37.0	19.0	37.0
5	31.892194190146036	37.0	33.0	37.0	19.0	37.0
6	32.04951954508958	37.0	35.0	37.0	17.0	37.0
7	32.27250360276151	37.0	35.0	37.0	17.0	37.0
8	32.53603238410033	37.0	35.0	37.0	17.0	37.0
9	34.270674475106695	39.0	37.0	39.0	17.0	39.0
10-11	34.47906322998192	39.0	37.0	39.0	20.0	39.0
12-13	34.49005793899497	39.0	37.0	39.0	22.5	39.0
14-15	35.89971598446867	40.5	38.0	41.0	25.0	41.0
16-17	35.91480639600722	41.0	38.0	41.0	25.0	41.0
18-19	35.938034236205894	41.0	38.0	41.0	25.0	41.0
20-21	35.92569792123696	41.0	38.0	41.0	25.0	41.0
22-23	35.87291312965952	41.0	38.0	41.0	24.0	41.0
24-25	35.81217018040687	41.0	38.0	41.0	23.0	41.0
26-27	35.731062740942804	40.5	38.0	41.0	23.0	41.0
28-29	35.661836393874175	40.0	37.0	41.0	22.0	41.0
30-31	35.57293034140826	40.0	37.0	41.0	20.5	41.0
32-33	35.457919442079216	40.0	37.0	41.0	18.5	41.0
34-35	35.37833808209961	40.0	37.0	41.0	18.0	41.0
36-37	35.30254634165798	40.0	36.5	41.0	17.0	41.0
38-39	35.207740604639625	40.0	36.0	41.0	16.0	41.0
40-41	35.08524064365871	40.0	36.0	41.0	15.0	41.0
42-43	34.9565568091759	40.0	35.5	41.0	11.0	41.0
44-45	34.819140288324974	40.0	35.0	41.0	8.5	41.0
46-47	34.62973258058307	40.0	35.0	41.0	6.0	41.0
48-49	34.437536526192204	40.0	35.0	41.0	2.0	41.0
50-51	34.26643830390139	40.0	35.0	41.0	2.0	41.0
52-53	34.08447760391647	39.0	35.0	41.0	2.0	41.0
54-55	33.88414195313895	39.0	35.0	41.0	2.0	41.0
56-57	33.64460838719411	39.0	35.0	41.0	2.0	41.0
58-59	33.575692892111384	38.5	35.0	41.0	2.0	41.0
60-61	33.335310877998616	38.0	34.5	41.0	2.0	41.0
62-63	33.04989044310065	37.0	34.0	40.0	2.0	41.0
64-65	32.74881707162689	37.0	34.0	40.0	2.0	41.0
66-67	32.430535419784576	36.0	34.0	39.0	2.0	41.0
68-69	32.097410260456684	36.0	34.0	39.0	2.0	41.0
70-71	31.763373572118528	35.0	33.5	38.5	2.0	40.5
72-73	31.42095580265711	35.0	33.0	37.0	2.0	39.5
74-75	31.088782708825768	35.0	33.0	37.0	2.0	39.0
76-77	30.79457235957901	35.0	33.0	36.0	2.0	39.0
78-79	30.539647935197117	35.0	33.0	36.0	2.0	37.5
80-81	30.312015404731888	35.0	33.0	35.5	2.0	37.0
82-83	30.112051085510743	35.0	32.5	35.0	2.0	37.0
84-85	29.944663794284487	35.0	32.0	35.0	2.0	36.0
86-87	29.804987852060467	35.0	32.0	35.0	2.0	36.0
88-89	29.687432258545613	35.0	32.0	35.0	2.0	36.0
90-91	29.587881151357603	35.0	32.0	35.0	2.0	35.0
92-93	29.521864340204736	35.0	32.0	35.0	2.0	35.0
94-95	29.455472945905683	35.0	32.0	35.0	2.0	35.0
96-97	29.39435519673419	35.0	32.0	35.0	2.0	35.0
98-99	29.340079876387563	35.0	32.0	35.0	2.0	35.0
100-101	29.28635450826424	35.0	32.0	35.0	2.0	35.0
102-103	28.609676991403663	34.0	30.0	35.0	2.0	35.0
104-105	29.160586482750965	35.0	31.0	35.0	2.0	35.0
106-107	29.222738237482247	35.0	32.0	35.0	2.0	35.0
108-109	29.29037672486001	35.0	32.0	35.0	2.0	35.0
110-111	29.399112272627164	35.0	32.0	35.0	2.0	35.0
112-113	29.3614027185025	35.0	32.0	35.0	2.0	35.0
114-115	29.298568346683638	35.0	32.0	35.0	2.0	35.0
116-117	29.233377439342675	35.0	32.0	35.0	2.0	35.0
118-119	29.155891620609705	35.0	32.0	35.0	2.0	35.0
120-121	29.05778140125312	35.0	32.0	35.0	2.0	35.0
122-123	28.939881789536297	35.0	32.0	35.0	2.0	35.0
124-125	28.416857498859777	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	164510.0
3	9538.0
4	7034.0
5	6602.0
6	6794.0
7	7039.0
8	7455.0
9	7710.0
10	8033.0
11	7863.0
12	8368.0
13	9431.0
14	10934.0
15	9226.0
16	6733.0
17	6047.0
18	5476.0
19	4923.0
20	4866.0
21	5004.0
22	5915.0
23	7320.0
24	9398.0
25	12100.0
26	14545.0
27	17151.0
28	20596.0
29	24408.0
30	29865.0
31	36799.0
32	47243.0
33	63566.0
34	96809.0
35	165633.0
36	372741.0
37	799873.0
38	278734.0
39	282.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.70830160734378	17.782855869880947	12.040415502413495	27.468427020361773
2	15.329251648772807	20.080344616494493	38.15909725461769	26.431306480115012
3	18.258413813794025	24.32007089332878	28.786801493476876	28.63471379940032
4	13.847075790859446	16.424908571037072	31.8469387666608	37.88107687144268
5	14.268452644654662	37.54380250002172	31.939123863132934	16.24862099219069
6	32.94181181721766	34.647225059806146	17.071483257531888	15.3394798654443
7	29.492596342946847	31.59723411775165	19.999262778328287	18.91090676097322
8	27.5560756659446	35.30048370844296	18.45662493257739	18.68681569303505
9	28.067633290105398	16.036219545876893	18.050632615021208	37.8455145489965
10-11	26.739050415282357	25.29162112514053	25.915249188923667	22.05407927065345
12-13	26.47627537547405	22.831989120497646	26.405133494053256	24.28660200997505
14-15	24.1719067842904	23.874949925516916	23.960314996679045	27.992828293513643
16-17	24.157556434592752	26.85698294085922	24.361366083923965	24.62409454062406
18-19	24.37660520150319	26.12684928751164	25.859438541484213	23.637106969500955
20-21	25.27608165218914	25.032320802717813	25.586239965593844	24.105357579499202
22-23	25.184950428429474	24.571895685530514	25.35594069793858	24.887213188101438
24-25	24.626609103410964	25.13151596920788	25.33528226400828	24.90659266337288
26-27	24.262539430945772	25.242178409096823	25.619059345415952	24.876222814541457
28-29	24.457916624034713	25.39931690601258	25.432981699185454	24.709784770767254
30-31	24.99208779812743	25.34902565027461	25.209554124663352	24.449332426934607
32-33	24.603674556613214	25.45645817761831	25.33712483156765	24.602742434200827
34-35	24.736426129949137	25.540565967386986	25.473669926349324	24.249337976314553
36-37	24.819319992855174	25.452599624376344	25.475750943828135	24.252329438940347
38-39	24.688107505362954	25.610800307296916	25.42793089634625	24.273161290993876
40-41	24.639745526245964	25.598422588751056	25.305692796731417	24.45613908827156
42-43	24.457786560442287	25.551426277354537	25.250090610969387	24.740696551233786
44-45	24.28315451034526	25.55127453649671	25.56477947284359	24.60079148031444
46-47	24.292107220957234	25.504126484242363	25.19093335368106	25.012832941119346
48-49	24.337802896429494	25.694019329184016	25.162622845063048	24.805554929323446
50-51	24.302815790066955	25.785454034659345	25.274455857283822	24.637274317989878
52-53	24.43784347627033	25.572149743081052	25.26715061884257	24.722856161806046
54-55	24.055001291965016	25.332550928567343	25.609911539415336	25.002536240052304
56-57	24.131587070638403	25.51184359072629	25.268798091013302	25.087771247622005
58-59	24.300387936341675	25.580235363076852	25.38084787588812	24.738528824693354
60-61	24.72333306164494	25.343714720250553	25.47241264495587	24.460539573148633
62-63	24.4417237067777	25.405299831264166	25.480498264951677	24.672478197006456
64-65	24.223325257829394	25.17998633465189	25.507313042256797	25.089375365261922
66-67	24.246064709238503	25.524156277475935	25.268863122809513	24.960915890476052
68-69	24.049603652879348	25.40863813013643	25.417439099890572	25.124319117093652
70-71	24.12475873203605	25.235892002129575	25.533802660580847	25.105546605253526
72-73	24.16893699893001	25.624890529809708	25.108624776940943	25.097547694319346
74-75	23.963761681878324	25.63119861404236	25.357414751986074	25.047624952093244
76-77	23.966016117480375	25.149638163085868	25.358541969787098	25.52580374964666
78-79	23.87824486985837	25.31271623072241	25.48078006940193	25.32825883001729
80-81	24.21398235644014	25.517327938873578	25.113350420799073	25.1553392838872
82-83	24.118537356865016	25.495152096364983	25.31995643736744	25.066354109402557
84-85	24.03761612511077	25.533477501599783	25.062603942487616	25.366302430801834
86-87	23.693944759391027	25.719771920484323	25.246080316869595	25.340203003255056
88-89	23.702117088448446	25.454463869201117	25.267302359700405	25.576116682650035
90-91	23.96360634643165	25.17723393665708	25.401117510262054	25.458042206649225
92-93	23.985150983069953	25.314582384947215	25.37449871019488	25.32576792178795
94-95	23.9387607652173	25.323484903170762	25.19604406647907	25.541710265132867
96-97	23.627955957589464	25.24942560304906	25.33394579218872	25.788672647172756
98-99	23.45720092128438	25.293526622408883	25.305362417016664	25.94391003929007
100-101	23.556012319623473	25.36528359932783	25.378029831385557	25.700674249663138
102-103	23.405810547637092	25.224966660365812	25.411499529169795	25.957723262827304
104-105	23.421346700989027	25.331765343449398	25.32008128979813	25.926806665763447
106-107	23.492423886062287	25.059217010971107	25.473297122097705	25.9750619808689
108-109	23.38683175030392	25.03349160737171	25.561510100808654	26.01816654151572
110-111	23.216740680557837	25.567156821728044	25.41012568993765	25.805976807776464
112-113	23.24001305853754	25.588317031116343	25.318041722287564	25.853628188058547
114-115	23.230682321195307	25.636176580377736	25.226149149835276	25.906991948591685
116-117	22.977952055057322	25.391348909178525	25.498132160206094	26.132566875558062
118-119	23.056658089661855	25.243512511082542	25.49789621318373	26.20193318607187
120-121	23.033188341267937	25.26176393187613	25.603809650982868	26.101238075873063
122-123	22.895488085656073	25.49205482789814	25.40751211833031	26.204944968115473
124-125	22.70713846284577	25.398309431226835	25.26605626298199	26.628495842945405
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	42.0
1	38.5
2	67.5
3	85.0
4	72.5
5	103.0
6	208.5
7	506.0
8	713.0
9	767.0
10	759.5
11	650.5
12	653.5
13	740.5
14	803.5
15	860.5
16	907.5
17	960.0
18	1042.0
19	1127.5
20	1203.0
21	1319.0
22	1404.5
23	1513.5
24	1755.0
25	2018.0
26	2470.0
27	3069.5
28	3865.0
29	5173.0
30	6967.5
31	9224.5
32	12406.5
33	15988.0
34	19679.0
35	24023.5
36	29302.0
37	34818.5
38	39797.5
39	45353.0
40	50859.0
41	55711.5
42	60862.5
43	67534.0
44	74567.5
45	80898.5
46	87424.5
47	93927.5
48	100552.0
49	106103.5
50	108740.5
51	110202.5
52	109466.0
53	107210.5
54	106119.0
55	103566.0
56	97029.0
57	87791.5
58	79889.0
59	71301.0
60	60195.0
61	50120.5
62	40819.0
63	31027.0
64	22621.5
65	16784.0
66	12770.0
67	9758.5
68	7442.5
69	5503.5
70	4031.5
71	3034.0
72	2199.5
73	1582.0
74	1211.5
75	898.5
76	674.5
77	480.0
78	364.0
79	312.0
80	263.0
81	218.5
82	200.5
83	184.0
84	171.5
85	172.5
86	167.0
87	151.0
88	144.5
89	126.0
90	112.0
91	114.0
92	100.0
93	85.5
94	90.5
95	78.5
96	55.0
97	50.5
98	39.0
99	15.5
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35715462480121946
2	0.0
3	0.0
4	0.017428521385055868
5	0.18139535690316852
6	0.05198208243950743
7	0.02640290926243538
8	0.009364578654656883
9	0.008020588199590386
10-11	0.0026446263793243976
12-13	0.05685946715547455
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	3.4683624646877345E-4
92-93	6.069634313203535E-4
94-95	1.5174085783008838E-4
96-97	6.286406967246519E-4
98-99	6.503179621289502E-5
100-101	0.0
102-103	0.0
104-105	6.503179621289502E-5
106-107	2.3844991944728177E-4
108-109	6.936724929375469E-4
110-111	6.503179621289503E-4
112-113	0.0011705723318321103
114-115	4.7689983889456355E-4
116-117	9.754769431934253E-4
118-119	3.901907772773702E-4
120-121	4.335453080859668E-5
122-123	0.0016257949053223755
124-125	1.3006359242579004E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2306564.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.64648002782287
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.4871282268896	49.41907494536701
2	9.637196641605856	11.689241072987025
3	3.255215876474618	5.922521539166095
4	1.6000354417326101	3.88146069843382
5	0.9651850165740374	2.9267536915405623
6	0.6256700639582983	2.2766812222712156
7	0.43589869822443	1.8505005187215322
8	0.32269959329285947	1.5656475551697568
9	0.25130446341926044	1.371665800949303
>10	1.3375080472135168	14.39868148976149
>50	0.06365902589576378	2.586408224832533
>100	0.018069514847038583	1.7574228624225259
>500	7.156497869697768E-5	0.022829262909510966
>1k	3.5782489348352075E-4	0.33111111546766825
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	8.670906161719336E-5
7	0.0	0.0	0.0	0.0	8.670906161719336E-5
8	0.0	0.0	0.0	0.0	8.670906161719336E-5
9	0.0	0.0	0.0	0.0	8.670906161719336E-5
10-11	0.0	0.0	0.0	0.0	8.670906161719336E-5
12-13	0.0	0.0	0.0	4.335453080859668E-5	2.3844991944728175E-4
14-15	0.0	2.167726540429834E-5	0.0	6.503179621289502E-5	2.601271848515801E-4
16-17	0.0	4.335453080859668E-5	0.0	8.670906161719336E-5	3.0348171566017677E-4
18-19	0.0	4.335453080859668E-5	0.0	8.670906161719336E-5	3.9019077727737013E-4
20-21	0.0	4.335453080859668E-5	0.0	1.3006359242579004E-4	3.9019077727737013E-4
22-23	0.0	4.335453080859668E-5	0.0	1.5174085783008838E-4	4.1186804268166844E-4
24-25	0.0	4.335453080859668E-5	0.0	3.251589810644751E-4	4.335453080859668E-4
26-27	0.0	4.335453080859668E-5	0.0	8.454133507676354E-4	5.202543697031602E-4
28-29	0.0	4.335453080859668E-5	0.0	0.002601271848515801	5.202543697031602E-4
30-31	0.0	4.335453080859668E-5	0.0	0.010947019029170663	5.202543697031602E-4
32-33	0.0	4.335453080859668E-5	0.0	0.028982503845546882	5.852861659160552E-4
34-35	0.0	4.335453080859668E-5	0.0	0.052350595951380495	6.069634313203535E-4
36-37	0.0	4.335453080859668E-5	0.0	0.08662235255557618	6.069634313203535E-4
38-39	0.0	4.335453080859668E-5	0.0	0.14346014244564642	6.069634313203535E-4
40-41	0.0	4.335453080859668E-5	0.0	0.21237216916591084	6.069634313203535E-4
42-43	0.0	4.335453080859668E-5	0.0	0.27775080162527466	6.069634313203535E-4
44-45	0.0	4.335453080859668E-5	0.0	0.34711805091902936	6.719952275332486E-4
46-47	0.0	4.335453080859668E-5	0.0	0.42268499811841337	6.936724929375469E-4
48-49	0.0	4.335453080859668E-5	0.0	0.5070962696027511	7.370270237461436E-4
50-51	0.0	4.335453080859668E-5	0.0	0.6005686380260855	7.370270237461436E-4
52-53	0.0	4.335453080859668E-5	0.0	0.6951031924542306	8.454133507676354E-4
54-55	0.0	4.335453080859668E-5	0.0	0.7941032635556611	9.53799677789127E-4
56-57	0.0	4.335453080859668E-5	0.0	0.8977856239844201	0.0010405087394063203
58-59	0.0	4.335453080859668E-5	0.0	1.018029415182063	0.001083863270214917
60-61	0.0	4.335453080859668E-5	0.0	1.1450148359204428	0.001083863270214917
62-63	0.0	4.335453080859668E-5	0.0	1.282860566626376	0.0012572813934493038
64-65	0.0	4.335453080859668E-5	0.0	1.4163708442514493	0.0012572813934493038
66-67	0.0	4.335453080859668E-5	0.0	1.562974190180719	0.0013006359242579005
68-69	0.0	4.335453080859668E-5	0.0	1.7253585853243179	0.0013439904550664973
70-71	0.0	4.335453080859668E-5	0.0	1.9034156433552245	0.0013873449858750938
72-73	0.0	4.335453080859668E-5	0.0	2.0801720654618734	0.001777535763152464
74-75	0.0	4.335453080859668E-5	0.0	2.2585976370046525	0.001777535763152464
76-77	0.0	4.335453080859668E-5	0.0	2.4589822784019866	0.001777535763152464
78-79	0.0	4.335453080859668E-5	0.0	2.684534224933711	0.001777535763152464
80-81	0.0	4.335453080859668E-5	0.0	2.9161124512478303	0.0017992130285567625
82-83	0.0	4.335453080859668E-5	0.0	3.1560147474772	0.0019292766209825524
84-85	0.0	4.335453080859668E-5	0.0	3.4078178624135296	0.001972631151791149
86-87	0.0	4.335453080859668E-5	0.0	3.6786102618440246	0.0019943084171954472
88-89	0.0	4.335453080859668E-5	0.0	3.981051468764795	0.002037662948004044
90-91	0.0	4.335453080859668E-5	0.0	4.3034791143883275	0.0020810174788126407
92-93	0.0	4.335453080859668E-5	0.0	4.63260503502179	0.0021243720096212376
94-95	0.0	4.335453080859668E-5	0.0	4.972027656722293	0.002297790132855624
96-97	0.0	4.335453080859668E-5	0.0	5.3388286646284255	0.002319467398259923
98-99	0.0	4.335453080859668E-5	0.0	5.73801550704858	0.002341144663664221
100-101	0.0	4.335453080859668E-5	0.0	6.176351490788896	0.002341144663664221
102-103	0.0	4.335453080859668E-5	0.0	6.629211242350093	0.002471208256090011
104-105	0.0	4.335453080859668E-5	0.0	7.102881168699415	0.0025145627868986076
106-107	0.0	4.335453080859668E-5	0.0	7.6060104987331805	0.0026012718485158006
108-109	0.0	4.335453080859668E-5	0.0	8.128757753957835	0.0027096581755372924
110-111	0.0	4.335453080859668E-5	0.0	8.693797353986277	0.002861399033367381
112-113	0.0	4.335453080859668E-5	0.0	9.273078917385341	0.0029047535641759776
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2390	0.0	61.772587	1
GTATCAA	4450	0.0	60.309128	1
TCAACGC	6265	0.0	40.907856	4
TATCAAC	6630	0.0	40.36381	2
GTACATG	11965	0.0	39.814598	1
CAACGCA	6590	0.0	38.438183	5
TACATGG	12395	0.0	38.131683	2
ATCAACG	6760	0.0	37.82422	3
AACGCAG	6775	0.0	36.855133	6
ACATGGG	12860	0.0	36.706547	3
CATGGGG	11855	0.0	34.63993	4
ATGGGGG	7420	0.0	32.371326	5
ACGCAGA	8215	0.0	30.31242	7
CGCAGAG	8830	0.0	27.857203	8
TGGGGGG	5045	0.0	27.050022	6
GTAAGTA	1240	0.0	25.474958	2
AGAGTAC	8690	0.0	24.500835	10-11
GCAGAGT	9960	0.0	23.739925	9
GTGGTAT	1225	0.0	23.420807	1
TAAGTAC	1665	0.0	22.552029	3
>>END_MODULE