FastQCFastQC Report
Mon 15 Aug 2016
SRR2921327_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921327_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2327244
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33500.057.90631
GGTATCA18300.053.977161
TCAACGC43100.044.0400474
CAACGCA44500.042.921025
GTACATG116950.042.587121
AACGCAG47450.040.2517246
TACATGG123350.039.652182
TATCAAC48000.039.5442962
ATCAACG48400.039.340423
ACATGGG125800.038.5015453
CATGGGG111650.037.625334
ATGGGGG67850.034.376475
ACGCAGA59250.032.2305037
TGGGGGG46050.031.6557166
GTATACG2109.276846E-1131.1692561
CGCAGAG63350.030.3323678
ATGGGGA40550.025.6786525
GCAGAGT76850.024.5389759
AGAGTAC64300.023.77733610-11
GTATAGG6650.022.370281
Produced by FastQC (version 0.11.5)