##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921327_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2327244 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.618810490004485 31.0 31.0 34.0 28.0 34.0 2 30.774891244751302 31.0 31.0 34.0 28.0 34.0 3 30.845808604512463 33.0 31.0 34.0 27.0 34.0 4 34.34999724996605 37.0 35.0 37.0 32.0 37.0 5 34.367836806110574 37.0 35.0 37.0 32.0 37.0 6 34.57131998191853 37.0 35.0 37.0 32.0 37.0 7 34.57945664485546 37.0 35.0 37.0 32.0 37.0 8 34.57365837015801 37.0 35.0 37.0 32.0 37.0 9 36.240416991084736 39.0 37.0 39.0 32.0 39.0 10-11 36.233327919204 39.0 37.0 39.0 32.0 39.0 12-13 36.16026660719719 39.0 37.0 39.0 32.0 39.0 14-15 37.51406599393961 41.0 38.0 41.0 32.0 41.0 16-17 37.499074012007334 41.0 38.0 41.0 32.0 41.0 18-19 37.49748071109003 41.0 38.5 41.0 32.0 41.0 20-21 37.52451204085176 41.0 38.5 41.0 32.0 41.0 22-23 37.47167035343092 41.0 38.0 41.0 32.0 41.0 24-25 37.44827358025201 41.0 38.0 41.0 32.0 41.0 26-27 37.407186569177966 41.0 38.0 41.0 31.0 41.0 28-29 37.338919339785605 41.0 38.0 41.0 31.0 41.0 30-31 37.26881839635208 41.0 38.0 41.0 31.0 41.0 32-33 37.19509127534543 40.5 38.0 41.0 31.0 41.0 34-35 37.09668797083589 40.0 38.0 41.0 30.0 41.0 36-37 37.00025330390797 40.0 38.0 41.0 30.0 41.0 38-39 36.91665313134334 40.0 38.0 41.0 30.0 41.0 40-41 36.82197010713101 40.0 38.0 41.0 30.0 41.0 42-43 36.654595306723316 40.0 37.0 41.0 30.0 41.0 44-45 36.439339837163615 40.0 37.0 41.0 30.0 41.0 46-47 36.24906391422644 40.0 36.5 41.0 29.0 41.0 48-49 36.0655034452769 40.0 36.0 41.0 28.5 41.0 50-51 35.9011738777713 40.0 35.5 41.0 28.0 41.0 52-53 35.719422415526694 39.5 35.0 41.0 28.0 41.0 54-55 35.514107244448795 39.0 35.0 41.0 28.0 41.0 56-57 35.27621190558446 39.0 35.0 41.0 27.0 41.0 58-59 35.01449396797241 38.5 35.0 40.5 26.5 41.0 60-61 34.73580316460156 38.0 35.0 40.0 26.0 41.0 62-63 34.4200902440827 37.5 34.0 40.0 26.0 41.0 64-65 34.074355546732534 37.0 34.0 40.0 26.0 41.0 66-67 33.73308729123375 36.0 34.0 39.0 25.5 41.0 68-69 33.37783360919612 36.0 34.0 39.0 25.0 40.5 70-71 33.30687370984735 35.5 34.0 39.0 26.0 40.0 72-73 33.00233108346181 35.0 34.0 37.5 25.0 40.0 74-75 32.66650510217235 35.0 34.0 37.0 25.0 39.0 76-77 32.354693147774796 35.0 33.5 37.0 24.0 39.0 78-79 32.05478003165976 35.0 33.0 36.0 24.0 38.5 80-81 31.771343915807712 35.0 33.0 36.0 23.0 37.0 82-83 31.54240573828958 35.0 33.0 35.0 22.5 37.0 84-85 31.32293197447281 35.0 33.0 35.0 21.0 36.5 86-87 31.150380235162277 35.0 33.0 35.0 20.0 36.0 88-89 31.0038689540074 35.0 33.0 35.0 20.0 36.0 90-91 30.881264491389814 35.0 33.0 35.0 19.5 35.5 92-93 30.768329620787505 35.0 33.0 35.0 18.5 35.0 94-95 30.645351110584024 35.0 32.0 35.0 18.0 35.0 96-97 30.551155787704253 35.0 32.0 35.0 17.0 35.0 98-99 30.46448803821172 35.0 32.0 35.0 15.5 35.0 100-101 30.365542246537103 35.0 32.0 35.0 11.0 35.0 102-103 29.538663328812966 34.0 30.5 35.0 8.0 35.0 104-105 30.118439665114614 34.5 31.5 35.0 7.0 35.0 106-107 30.183755549482562 35.0 32.0 35.0 6.0 35.0 108-109 30.112094391477644 35.0 32.0 35.0 2.0 35.0 110-111 30.026376692774804 35.0 32.0 35.0 2.0 35.0 112-113 29.930251404665775 35.0 32.0 35.0 2.0 35.0 114-115 29.803730077293142 35.0 31.5 35.0 2.0 35.0 116-117 29.668754758847804 35.0 31.0 35.0 2.0 35.0 118-119 29.66224770587012 35.0 31.5 35.0 2.0 35.0 120-121 29.814056884452167 35.0 32.0 35.0 2.0 35.0 122-123 29.67882933633087 35.0 32.0 35.0 2.0 35.0 124-125 29.136461411008042 34.5 31.0 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 67119.0 3 9692.0 4 6096.0 5 5211.0 6 5721.0 7 6172.0 8 6554.0 9 7022.0 10 7511.0 11 8051.0 12 8010.0 13 7837.0 14 7879.0 15 7239.0 16 6354.0 17 6062.0 18 5710.0 19 5506.0 20 5733.0 21 5973.0 22 6876.0 23 8805.0 24 11950.0 25 15455.0 26 17287.0 27 20183.0 28 24247.0 29 29403.0 30 35922.0 31 44519.0 32 56807.0 33 76648.0 34 118219.0 35 196606.0 36 431184.0 37 797476.0 38 240111.0 39 94.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.70234565036143 17.663331700231215 12.409298367757406 25.22502428164995 2 16.08033808830898 19.633978321986657 39.83199582632492 24.45368776337944 3 19.101003364028447 24.96961716771839 28.73008355923492 27.199295909018247 4 13.354376932548032 15.854812058025324 35.84870955750425 34.9421014519224 5 14.620492453509648 37.67830576328231 33.0656687760685 14.635533007139538 6 32.92155917125937 34.56887073300099 18.174800280934853 14.334769814804785 7 29.435616089842792 31.683748698380555 21.037264456839523 17.84337075493713 8 27.161112987692775 34.97674614491393 19.649657960760248 18.212482906633042 9 28.206412997889153 15.842466601117994 18.70371770983024 37.24740269116262 10-11 26.402251096240555 25.29917784107219 27.205072186000777 21.093498876686475 12-13 26.421133985066668 22.802258541994643 27.234171287450152 23.54243618548853 14-15 24.11047626905386 23.902491010223937 24.445637638112526 27.541395082609682 16-17 24.310061290165944 26.816327600513425 24.95003495726705 23.923576152053585 18-19 24.37891394972182 26.51582897632368 25.997671773324054 23.107585300630443 20-21 25.373274788514145 25.619460394154803 25.47544031084102 23.531824506490036 22-23 25.227975999405274 25.138487281859167 25.469713709155865 24.163823009579698 24-25 24.595333595175457 25.335675015534342 25.527974278738103 24.541017110552094 26-27 24.35720151310907 25.519298672312534 25.628166723890256 24.49533309068814 28-29 24.469301806820035 25.917649846944663 25.459375501563215 24.15367284467209 30-31 25.03634337712587 25.61188582056105 25.325908670371692 24.02586213194139 32-33 24.74468671184847 25.630252642516705 25.31801088333993 24.307049762294895 34-35 24.71752948036941 25.764667560786002 25.49881674578663 24.018986213057953 36-37 24.718873465557056 25.67213392314614 25.493840741947153 24.115151869349642 38-39 24.66849371580326 25.8651715087766 25.372964041653283 24.093370733766857 40-41 24.715816370051403 25.536572856614598 25.494738963598067 24.252871809735932 42-43 24.508222363936078 25.60589119011563 25.369477815001538 24.516408630946753 44-45 24.365499575216358 25.581429327131534 25.63617635418318 24.416894743468927 46-47 24.419857379457106 25.413942503139214 25.583730910084768 24.58246920731891 48-49 24.32410887827836 25.671937599670652 25.550903959925037 24.453049562125948 50-51 24.290629663800296 25.711046242553497 25.611200898629583 24.387123195016628 52-53 24.401843146877216 25.626819050434722 25.51981924337815 24.451518559309907 54-55 24.19464833532366 25.367615935617415 25.81802844669886 24.619707282360064 56-57 24.35183842290441 25.540611822845328 25.50071173421427 24.60683802003599 58-59 24.384380241982875 25.614341323869056 25.626846343603134 24.374432090544936 60-61 24.727022397699468 25.5980583675746 25.574380971452193 24.100538263273737 62-63 24.576125883393367 25.632330456799128 25.573244192572233 24.21829946723527 64-65 24.349295694758162 25.402514619267375 25.639929329912558 24.608260356061905 66-67 24.436959825846543 25.54299413486838 25.540781120885136 24.47926491839994 68-69 24.235164543803826 25.50063158401357 25.661927763290176 24.602276108892433 70-71 24.215108665361907 25.371752183974923 25.804411157732847 24.60872799293032 72-73 24.27382321578472 25.746706306226557 25.465351946480148 24.514118531508572 74-75 24.17355118867984 25.71082407787583 25.606683395351503 24.50894133809282 76-77 24.245873024603718 25.275661673960347 25.61249316754386 24.865972133892075 78-79 24.090038540555245 25.452966471134975 25.751506450569128 24.705488537740653 80-81 24.333111246564226 25.640644414016435 25.43795174264347 24.58829259677587 82-83 24.404824444079185 25.492032490978605 25.69350577917006 24.409637285772146 84-85 24.35394066595717 25.64965837083315 25.40393547689816 24.59246548631152 86-87 24.072295095105506 25.779718603645307 25.643560463388887 24.5044258378603 88-89 24.059526643593856 25.446903175848668 25.752945908386565 24.740624272170912 90-91 24.30349291310431 25.348973319719892 25.77759471826023 24.569939048915565 92-93 24.38990745557294 25.44014521424064 25.654601792101527 24.515345538084894 94-95 24.34289191372074 25.342001739095682 25.64850285004135 24.666603497142223 96-97 24.09674538050993 25.308619560118963 25.81366561589298 24.78096944347814 98-99 24.022367134707736 25.346733560370854 25.633509878280673 24.99738942664074 100-101 24.021379955088857 25.448687054946856 25.74331033057239 24.786622659391902 102-103 23.921075463160513 25.34421464572566 25.850658477965577 24.884051413148246 104-105 24.02882186666621 25.435580945047143 25.683763034340274 24.851834153946374 106-107 24.04305341110232 25.240915265293633 25.889071331545388 24.826959992058658 108-109 24.00237981105469 25.22954240204067 25.863557069807147 24.90452071709749 110-111 23.897435941510572 25.606593403760037 25.801668462213794 24.6943021925156 112-113 23.861916519775136 25.638344318327338 25.811906937090317 24.687832224807206 114-115 23.948530939424874 25.729674634735666 25.706062411467588 24.615732014371872 116-117 23.805040520411033 25.601629109810002 25.837624865152186 24.755705504626775 118-119 23.84781423781129 25.432836925720043 25.868089290106344 24.85125954636233 120-121 23.76990672021824 25.471706221545716 26.09341386123977 24.664973196996275 122-123 23.706998883848243 25.676904682358682 25.847711351768275 24.7683850820248 124-125 23.690894637332416 25.58776641457546 25.847241320041253 24.874097628050876 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 252.0 1 211.0 2 163.5 3 149.5 4 128.5 5 113.0 6 120.5 7 159.5 8 218.5 9 224.5 10 198.0 11 207.5 12 230.0 13 257.5 14 286.0 15 328.0 16 373.5 17 393.5 18 436.0 19 466.5 20 538.0 21 676.5 22 745.0 23 853.0 24 1074.0 25 1372.5 26 1826.0 27 2467.0 28 3437.0 29 4897.0 30 7251.5 31 10143.5 32 13924.5 33 18318.0 34 23444.0 35 28973.0 36 34602.0 37 40712.5 38 46337.0 39 52351.0 40 58110.5 41 63293.5 42 68499.5 43 74631.5 44 81239.5 45 86453.5 46 91748.0 47 96927.5 48 102065.0 49 106780.0 50 108418.0 51 109235.5 52 107757.5 53 104322.5 54 102252.0 55 98436.0 56 92206.5 57 84024.5 58 75541.0 59 66462.5 60 55950.0 61 46656.5 62 38283.5 63 29335.0 64 21047.5 65 15241.5 66 11383.5 67 8674.5 68 6474.0 69 4760.0 70 3549.0 71 2600.0 72 1887.0 73 1335.5 74 941.0 75 628.0 76 415.5 77 257.0 78 159.5 79 115.0 80 92.5 81 60.0 82 46.5 83 36.0 84 31.5 85 27.5 86 19.5 87 18.0 88 14.5 89 13.0 90 11.5 91 10.0 92 8.5 93 4.0 94 2.5 95 4.0 96 3.5 97 1.5 98 1.5 99 1.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016500203674389105 2 0.01194545995177128 3 0.011687644269358949 4 0.014136893252276083 5 0.008679794641215103 6 0.031195697571891903 7 0.014394708934688413 8 0.013664231167853478 9 0.009453241688452092 10-11 0.008593856080410993 12-13 0.007197354467344206 14-15 0.0067032077427205735 16-17 0.0050274058070404306 18-19 0.006488361340710299 20-21 0.005929760695483585 22-23 0.00580085285427742 24-25 0.006101637817091805 26-27 0.004941467246236321 28-29 0.006209061018096942 30-31 0.00580085285427742 32-33 0.008636825360813049 34-35 0.008035255435184279 36-37 0.007648531911565783 38-39 0.0075840779909627015 40-41 0.007884862953777086 42-43 0.007562593350761673 44-45 0.007605562631163728 46-47 0.009882934492472642 48-49 0.008808702482421268 50-51 0.00623054565829797 52-53 0.00588679141508153 54-55 0.006660238462318518 56-57 0.007798924392972976 58-59 0.007670016551766811 60-61 0.005586006452267145 62-63 0.005929760695483585 64-65 0.003974658437190084 66-67 0.0043398973206075515 68-69 0.006810630943725711 70-71 0.005263736849251733 72-73 0.004619197643220909 74-75 0.004447320521612689 76-77 0.004984436526638375 78-79 0.0036523888341746715 80-81 0.0032441806703551496 82-83 0.0058653067748805025 84-85 0.009990357693477779 86-87 0.007326262308550372 88-89 0.005070375087442486 90-91 0.005671945013071255 92-93 0.008572371440209964 94-95 0.007412200869354481 96-97 0.009818480571869558 98-99 0.007261808387947288 100-101 0.00878721784222024 102-103 0.00580085285427742 104-105 0.005242252209050706 106-107 0.007777439752771949 108-109 0.0072403237477462615 110-111 0.008207132556792498 112-113 0.005586006452267145 114-115 0.00255667218392227 116-117 0.0035879349135715896 118-119 0.15681638882729956 120-121 0.01142982858694662 122-123 0.0022129179407058305 124-125 0.0018906483376904181 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2327244.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.71095399183004 #Duplication Level Percentage of deduplicated Percentage of total 1 80.34887581498042 48.78056902898542 2 10.493906905454791 12.74190198663227 3 3.4077588096075924 6.206648649160202 4 1.6742177673538088 4.06573431384486 5 0.9832943276474551 2.984836834311605 6 0.6570028263995785 2.3932361019628265 7 0.42908759684857495 1.8235222145517125 8 0.3323574392049798 1.6142189760332784 9 0.253122775686358 1.3830592670080917 >10 1.3517978875348375 14.34032721037167 >50 0.05444539222825122 2.213697674243097 >100 0.013849075918040714 1.276290365070101 >500 1.4169056766089223E-4 0.05290334263617075 >1k 1.4169056766087938E-4 0.12305403518867548 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.296928040205496E-5 7 0.0 0.0 0.0 0.0 4.296928040205496E-5 8 0.0 0.0 0.0 0.0 4.296928040205496E-5 9 0.0 0.0 0.0 0.0 8.593856080410993E-5 10-11 0.0 0.0 0.0 0.0 8.593856080410993E-5 12-13 0.0 0.0 0.0 0.0 8.593856080410993E-5 14-15 0.0 0.0 0.0 4.296928040205496E-5 1.2890784120616488E-4 16-17 0.0 0.0 0.0 4.296928040205496E-5 1.7187712160821985E-4 18-19 0.0 0.0 0.0 4.296928040205496E-5 1.7187712160821985E-4 20-21 0.0 0.0 0.0 4.296928040205496E-5 1.7187712160821985E-4 22-23 4.296928040205496E-5 0.0 0.0 6.445392060308244E-5 2.7930032261335727E-4 24-25 4.296928040205496E-5 0.0 0.0 1.2890784120616488E-4 3.8672352361849465E-4 26-27 4.296928040205496E-5 0.0 0.0 5.80085285427742E-4 4.0820816381952215E-4 28-29 4.296928040205496E-5 0.0 0.0 0.0015683787346750062 4.2969280402054965E-4 30-31 4.296928040205496E-5 0.0 0.0 0.009195426006039762 4.2969280402054965E-4 32-33 4.296928040205496E-5 0.0 0.0 0.027414400896511065 4.726620844226046E-4 34-35 4.296928040205496E-5 0.0 0.0 0.047352147003064574 6.875084864328794E-4 36-37 4.296928040205496E-5 0.0 0.0 0.07760252040611126 6.875084864328794E-4 38-39 4.296928040205496E-5 0.0 0.0 0.13391376237300429 6.875084864328794E-4 40-41 4.296928040205496E-5 0.0 0.0 0.20004348491176688 7.734470472369893E-4 42-43 4.296928040205496E-5 0.0 0.0 0.26413216663143185 7.734470472369893E-4 44-45 4.296928040205496E-5 0.0 0.0 0.3312286979792407 7.734470472369893E-4 46-47 4.296928040205496E-5 0.0 0.0 0.40189167960042005 7.734470472369893E-4 48-49 4.296928040205496E-5 0.0 0.0 0.47973053104874264 8.164163276390443E-4 50-51 4.296928040205496E-5 0.0 0.0 0.5641221977583786 8.164163276390443E-4 52-53 4.296928040205496E-5 0.0 0.0 0.6524240689846015 8.593856080410993E-4 54-55 4.296928040205496E-5 0.0 0.0 0.7480307178791739 9.023548884431542E-4 56-57 4.296928040205496E-5 0.0 0.0 0.8467741242430962 9.023548884431542E-4 58-59 4.296928040205496E-5 8.593856080410993E-5 0.0 0.9575704137597949 9.023548884431542E-4 60-61 4.296928040205496E-5 8.593856080410993E-5 0.0 1.0740171636493638 9.023548884431542E-4 62-63 4.296928040205496E-5 8.593856080410993E-5 0.0 1.2064914551288992 9.023548884431542E-4 64-65 4.296928040205496E-5 8.593856080410993E-5 0.0 1.332262538865714 9.238395286441817E-4 66-67 4.296928040205496E-5 8.593856080410993E-5 0.0 1.465016130667863 9.453241688452092E-4 68-69 4.296928040205496E-5 8.593856080410993E-5 0.0 1.6189106084278229 9.453241688452092E-4 70-71 4.296928040205496E-5 8.593856080410993E-5 0.0 1.788811143137548 9.88293449247264E-4 72-73 4.296928040205496E-5 8.593856080410993E-5 0.0 1.953684272040233 0.0013105630522626762 74-75 4.296928040205496E-5 8.593856080410993E-5 0.0 2.1251317008444324 0.0013750169728657588 76-77 4.296928040205496E-5 8.593856080410993E-5 0.0 2.314475834936087 0.0014179862532678138 78-79 4.296928040205496E-5 8.593856080410993E-5 0.0 2.5269159572438475 0.0014609555336698688 80-81 4.296928040205496E-5 8.593856080410993E-5 0.0 2.744834662802869 0.0015039248140719238 82-83 4.296928040205496E-5 1.0742320100513741E-4 0.0 2.978114886105625 0.0015468940944739786 84-85 4.296928040205496E-5 1.2890784120616488E-4 0.0 3.2157779760093916 0.0015468940944739786 86-87 4.296928040205496E-5 1.2890784120616488E-4 0.0 3.4700916620689535 0.0015468940944739786 88-89 4.296928040205496E-5 1.2890784120616488E-4 0.0 3.756331523467243 0.0015468940944739786 90-91 4.296928040205496E-5 1.2890784120616488E-4 0.0 4.0577825101278595 0.0015468940944739786 92-93 4.296928040205496E-5 1.2890784120616488E-4 0.0 4.362563616019635 0.0015468940944739786 94-95 4.296928040205496E-5 1.2890784120616488E-4 0.0 4.683716017744594 0.0015898633748760336 96-97 4.296928040205496E-5 1.2890784120616488E-4 0.0 5.030413656668575 0.001611348015077061 98-99 4.296928040205496E-5 1.2890784120616488E-4 0.0 5.405320628176504 0.0016328326552780886 100-101 4.296928040205496E-5 1.2890784120616488E-4 0.0 5.805192751598027 0.0016328326552780886 102-103 4.296928040205496E-5 1.7187712160821985E-4 0.0 6.224938167205501 0.0016543172954791162 104-105 4.296928040205496E-5 1.7187712160821985E-4 0.0 6.665287352765761 0.0016758019356801436 106-107 4.296928040205496E-5 1.7187712160821985E-4 0.0 7.135091980041629 0.0016758019356801436 108-109 4.296928040205496E-5 1.7187712160821985E-4 0.0 7.6267250017617405 0.0016758019356801436 110-111 4.296928040205496E-5 1.7187712160821985E-4 0.0 8.144375922765295 0.0017187712160821986 112-113 4.296928040205496E-5 1.7187712160821985E-4 0.0 8.682759521562843 0.0017617404964842534 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3350 0.0 57.9063 1 GGTATCA 1830 0.0 53.97716 1 TCAACGC 4310 0.0 44.040047 4 CAACGCA 4450 0.0 42.92102 5 GTACATG 11695 0.0 42.58712 1 AACGCAG 4745 0.0 40.251724 6 TACATGG 12335 0.0 39.65218 2 TATCAAC 4800 0.0 39.544296 2 ATCAACG 4840 0.0 39.34042 3 ACATGGG 12580 0.0 38.501545 3 CATGGGG 11165 0.0 37.62533 4 ATGGGGG 6785 0.0 34.37647 5 ACGCAGA 5925 0.0 32.230503 7 TGGGGGG 4605 0.0 31.655716 6 GTATACG 210 9.276846E-11 31.169256 1 CGCAGAG 6335 0.0 30.332367 8 ATGGGGA 4055 0.0 25.678652 5 GCAGAGT 7685 0.0 24.538975 9 AGAGTAC 6430 0.0 23.777336 10-11 GTATAGG 665 0.0 22.37028 1 >>END_MODULE