FastQCFastQC Report
Mon 15 Aug 2016
SRR2921327_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921327_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2327244
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21350.062.7056271
GTATCAA39300.054.139451
GTACATG109800.042.837961
TACATGG115950.040.6021542
ACATGGG118950.039.127253
TCAACGC52500.038.1392944
CATGGGG106400.037.469444
CAACGCA55800.036.3109365
TATCAAC59150.035.463422
ATCAACG56800.035.4618233
AACGCAG58550.034.2920076
ATGGGGG63350.033.3944635
TGGGGGG46300.028.8241796
ACGCAGA70550.028.1997957
CGCAGAG76500.026.0804128
ATGGGGA38750.024.9135725
AGAGTAC77750.023.36134310-11
GCAGAGT87500.021.8478859
CAGAGTA84450.020.309789
ATTACGC1550.005638955619.2234363
Produced by FastQC (version 0.11.5)