##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921327_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2327244
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.04996596832992	31.0	30.0	34.0	16.0	34.0
2	29.847786050796564	33.0	31.0	34.0	25.0	34.0
3	30.111947866231475	33.0	31.0	34.0	26.0	34.0
4	32.248482754709	37.0	35.0	37.0	19.0	37.0
5	31.853421901614098	37.0	33.0	37.0	19.0	37.0
6	32.0088701485534	37.0	35.0	37.0	17.0	37.0
7	32.23332448166157	37.0	35.0	37.0	17.0	37.0
8	32.49760790016002	37.0	35.0	37.0	17.0	37.0
9	34.237171521335966	39.0	37.0	39.0	17.0	39.0
10-11	34.44357531913285	39.0	37.0	39.0	19.5	39.0
12-13	34.46586756695903	39.0	37.0	39.0	22.5	39.0
14-15	35.88299013769076	40.5	38.0	41.0	24.5	41.0
16-17	35.89346024739993	41.0	38.0	41.0	24.5	41.0
18-19	35.915664193354885	41.0	38.0	41.0	24.5	41.0
20-21	35.90143083406811	41.0	38.0	41.0	24.0	41.0
22-23	35.84802968661644	41.0	38.0	41.0	23.5	41.0
24-25	35.79846848890791	41.0	38.0	41.0	23.0	41.0
26-27	35.71951823702199	40.5	38.0	41.0	22.0	41.0
28-29	35.650667484801765	40.0	37.5	41.0	21.5	41.0
30-31	35.5679606435767	40.0	37.0	41.0	19.5	41.0
32-33	35.45730486360691	40.0	37.0	41.0	18.0	41.0
34-35	35.380036429355926	40.0	37.0	41.0	17.5	41.0
36-37	35.30455788048009	40.0	37.0	41.0	16.0	41.0
38-39	35.213175756388246	40.0	36.5	41.0	15.5	41.0
40-41	35.100874682671865	40.0	36.0	41.0	14.5	41.0
42-43	34.978123694808104	40.0	36.0	41.0	9.5	41.0
44-45	34.85060096835571	40.0	35.5	41.0	8.5	41.0
46-47	34.673052331427215	40.0	35.0	41.0	6.0	41.0
48-49	34.493701777725065	40.0	35.0	41.0	2.0	41.0
50-51	34.331140825800816	40.0	35.0	41.0	2.0	41.0
52-53	34.15197998147165	39.5	35.0	41.0	2.0	41.0
54-55	33.955072824336426	39.0	35.0	41.0	2.0	41.0
56-57	33.71659868926507	39.0	35.0	41.0	2.0	41.0
58-59	33.65696764069432	39.0	35.0	41.0	2.0	41.0
60-61	33.42407199245116	38.0	34.5	41.0	2.0	41.0
62-63	33.144177834382646	37.0	34.0	40.5	2.0	41.0
64-65	32.8461650346934	37.0	34.0	40.0	2.0	41.0
66-67	32.52696193437388	36.0	34.0	39.5	2.0	41.0
68-69	32.19059002837692	36.0	34.0	39.0	2.0	41.0
70-71	31.85720319828948	35.0	33.5	39.0	2.0	40.5
72-73	31.512891643506226	35.0	33.0	37.0	2.0	39.5
74-75	31.177868113528277	35.0	33.0	37.0	2.0	39.0
76-77	30.87329454926084	35.0	33.0	36.5	2.0	39.0
78-79	30.610968811177514	35.0	33.0	36.0	2.0	38.0
80-81	30.378301114966888	35.0	33.0	36.0	2.0	37.0
82-83	30.172434433175034	35.0	32.5	35.0	2.0	37.0
84-85	29.999415617786532	35.0	32.0	35.0	2.0	36.0
86-87	29.852526206964114	35.0	32.0	35.0	2.0	36.0
88-89	29.730380656261225	35.0	32.0	35.0	2.0	36.0
90-91	29.628528852153018	35.0	32.0	35.0	2.0	35.5
92-93	29.56358787475658	35.0	32.0	35.0	2.0	35.0
94-95	29.494488330402827	35.0	32.0	35.0	2.0	35.0
96-97	29.433953423018814	35.0	32.0	35.0	2.0	35.0
98-99	29.378642720746086	35.0	32.0	35.0	2.0	35.0
100-101	29.32330172513067	35.0	32.0	35.0	2.0	35.0
102-103	28.648632459681927	34.0	30.0	35.0	2.0	35.0
104-105	29.19987719379661	35.0	31.0	35.0	2.0	35.0
106-107	29.26212893877909	35.0	32.0	35.0	2.0	35.0
108-109	29.32906197201497	35.0	32.0	35.0	2.0	35.0
110-111	29.43761977686912	35.0	32.0	35.0	2.0	35.0
112-113	29.40061871466851	35.0	32.0	35.0	2.0	35.0
114-115	29.340836199384334	35.0	32.0	35.0	2.0	35.0
116-117	29.27712156525057	35.0	32.0	35.0	2.0	35.0
118-119	29.19980844294797	35.0	32.0	35.0	2.0	35.0
120-121	29.109669205291752	35.0	32.0	35.0	2.0	35.0
122-123	28.99797872504989	35.0	32.0	35.0	2.0	35.0
124-125	28.478867707898267	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	167980.0
3	9751.0
4	7143.0
5	6660.0
6	6722.0
7	7142.0
8	7615.0
9	7747.0
10	7926.0
11	7800.0
12	8245.0
13	9004.0
14	10119.0
15	8785.0
16	6400.0
17	5861.0
18	5335.0
19	4958.0
20	4744.0
21	4944.0
22	5723.0
23	7254.0
24	9327.0
25	12120.0
26	14658.0
27	17195.0
28	20688.0
29	24228.0
30	29439.0
31	36372.0
32	46612.0
33	62832.0
34	95859.0
35	162096.0
36	368216.0
37	811970.0
38	297435.0
39	339.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.53092818176958	17.928773341657887	11.980196995227756	27.560101481344784
2	15.14744478877161	19.918109145409762	38.4764554124965	26.457990653322128
3	18.021788862706273	24.525619144361315	28.836555169977878	28.616036822954534
4	13.560460557728515	16.707272681170227	32.23031668393622	37.501950077165034
5	14.189513775416208	37.42525151477063	32.17348708032804	16.211747629485124
6	32.610351711958344	35.0435147465208	17.29416690884516	15.051966632675699
7	29.227793038029905	31.495713399714354	20.456627049227887	18.819866513027854
8	27.22991823074503	35.53017658514952	18.577366228971105	18.66253895513434
9	27.69456870657092	16.304494493074984	18.259656993558423	37.74127980679567
10-11	26.423762588961996	25.480821807439234	26.20046730226937	21.894948301329396
12-13	26.239987170507987	23.04473855395631	26.515344012251653	24.19993026328405
14-15	23.969875956281335	24.107850315652335	24.069736563935713	27.85253716413062
16-17	23.964225495908465	26.973170840702565	24.521042915998496	24.541560747390477
18-19	24.219957168221296	26.312045492436546	25.817082351485276	23.65091498785688
20-21	25.146783061853416	25.32933804964155	25.468537033504006	24.05534185500102
22-23	24.93122766671651	24.90970005723508	25.3157812416747	24.843291034373706
24-25	24.430721488593374	25.322355541576215	25.35527001036419	24.891652959466217
26-27	24.16085292302827	25.39291109999639	25.54285240395936	24.90338357301598
28-29	24.342462586647553	25.5791829305393	25.451027051740173	24.627327431072978
30-31	24.808657794369648	25.525149060433716	25.276571773307825	24.389621371888808
32-33	24.517261619323115	25.676336473528345	25.319412145868675	24.486989761279865
34-35	24.592973491391533	25.71180761450024	25.457601351641685	24.237617542466538
36-37	24.765838906449	25.47556251084974	25.517629436363354	24.2409691463379
38-39	24.461788278324057	25.85003978955365	25.420948555458732	24.267223376663555
40-41	24.462905479614513	25.837277913274242	25.369192057214455	24.330624549896786
42-43	24.333890215207344	25.785886653913387	25.224600428661542	24.65562270221773
44-45	24.201931555092635	25.742444711426906	25.54287388859956	24.5127498448809
46-47	24.17110109640416	25.702698127055008	25.23905959151683	24.887141185024003
48-49	24.2493481560163	25.871932637918498	25.16205864103635	24.71666056502885
50-51	24.141946439651367	25.9472577864633	25.37076043594913	24.540035337936203
52-53	24.27353986088266	25.814418256100346	25.292491891696788	24.619549991320206
54-55	24.00599163645926	25.569149603565418	25.6209490710901	24.803909688885224
56-57	24.096828695229206	25.734495394552525	25.260630170278663	24.908045739939602
58-59	24.180833638415226	25.70647942373039	25.411430859849677	24.701256078004715
60-61	24.472380205943168	25.634140640173523	25.452982153998466	24.440496999884843
62-63	24.263033871824355	25.72753437112739	25.444216420796444	24.565215336251807
64-65	24.12630562158502	25.4662166923623	25.42722207039743	24.980255615655256
66-67	24.115155093320684	25.665851969110243	25.365582637660683	24.85341029990839
68-69	23.895904340069198	25.625331937691104	25.4600506006246	25.018713121615093
70-71	23.99065160335573	25.458116983006505	25.57445630969507	24.97677510394269
72-73	23.993122336978846	25.763478174183714	25.2153405487349	25.028058940102543
74-75	23.886408129100346	25.7548413488229	25.367301408876763	24.99144911319999
76-77	23.89053318001894	25.35522704108379	25.39145014446272	25.362789634434552
78-79	23.75627566340272	25.46451940578642	25.516318873311093	25.262886057499774
80-81	23.939990821761707	25.692106199435898	25.21074283573188	25.157160143070517
82-83	23.968866178191885	25.614482194389588	25.381073063245623	25.0355785641729
84-85	23.897257872401862	25.656140911739378	25.193404731089647	25.25319648476911
86-87	23.6033050251714	25.870428713104427	25.339242468774227	25.18702379294994
88-89	23.58540831988395	25.55982526971817	25.432356899405477	25.4224095109924
90-91	23.860495694470703	25.365927020848734	25.507102834258603	25.26647445042196
92-93	23.842870153394514	25.473195760891308	25.435382639781253	25.248551445932932
94-95	23.757504324325602	25.44166559422389	25.328398449408883	25.472431632041626
96-97	23.60124783272854	25.342154960715185	25.4432405837829	25.613356622773374
98-99	23.454180137536074	25.389022380120004	25.34121905567272	25.815578426671205
100-101	23.447949591877776	25.50037727028193	25.45558179546279	25.59609134237751
102-103	23.27132436478513	25.366012330464706	25.519627507902047	25.843035796848117
104-105	23.369401747302817	25.462091641443703	25.364916613814454	25.80358999743903
106-107	23.377961285487835	25.27172733722034	25.47871062773473	25.871600749557093
108-109	23.284895287710647	25.210550378689895	25.542382072451673	25.96217226114778
110-111	23.16929747103322	25.616385968756916	25.492462084834578	25.721854475375284
112-113	23.158583608321244	25.672781320745514	25.43511613731986	25.733518933613382
114-115	23.13312537584781	25.67081113517158	25.364632804072297	25.831430684908312
116-117	22.973637549009435	25.520570480663608	25.497431388940633	26.00836058138633
118-119	23.005415430043925	25.319982176304194	25.534592708355262	26.14000968529661
120-121	22.903827792726325	25.39205205131224	25.635065025467913	26.069055130493517
122-123	22.86441829052054	25.542522995927946	25.42343166514517	26.16962704840634
124-125	22.634735273271186	25.496602305005567	25.32122284792777	26.54743957379548
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	58.0
1	62.0
2	68.5
3	73.0
4	79.0
5	96.5
6	179.5
7	389.5
8	553.5
9	603.5
10	577.5
11	520.0
12	561.0
13	607.5
14	625.0
15	733.0
16	856.5
17	887.5
18	906.5
19	1006.0
20	1124.5
21	1196.5
22	1317.5
23	1462.5
24	1663.5
25	1978.5
26	2490.5
27	3133.5
28	4024.5
29	5414.5
30	7351.0
31	9786.0
32	13276.0
33	17262.0
34	21400.0
35	26187.5
36	31512.0
37	37523.5
38	43204.5
39	48981.5
40	55061.0
41	60273.5
42	65165.5
43	71340.0
44	77848.0
45	83681.0
46	89549.5
47	94908.5
48	100312.0
49	105134.5
50	107488.0
51	109311.0
52	109259.5
53	106997.5
54	105226.0
55	101472.0
56	94952.0
57	86484.5
58	77990.5
59	68765.0
60	58034.5
61	48469.0
62	39597.5
63	30340.0
64	22110.0
65	16357.0
66	12376.0
67	9394.5
68	7110.0
69	5330.5
70	3954.5
71	2931.0
72	2153.0
73	1583.5
74	1214.5
75	899.5
76	656.0
77	495.0
78	390.0
79	308.5
80	252.0
81	226.5
82	210.5
83	186.0
84	179.0
85	180.5
86	164.5
87	156.0
88	146.5
89	134.5
90	132.5
91	122.5
92	105.0
93	99.0
94	89.0
95	76.0
96	62.0
97	47.5
98	31.5
99	13.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3437542432164397
2	0.0
3	0.0
4	0.017746312806048697
5	0.17703343525646645
6	0.04881310253673444
7	0.0247073362311816
8	0.008937610323627432
9	0.007476654789957564
10-11	0.0024277643427161053
12-13	0.05813743638398036
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	1.7187712160821985E-4
92-93	4.0820816381952215E-4
94-95	1.0742320100513741E-4
96-97	3.8672352361849465E-4
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.0
106-107	1.2890784120616488E-4
108-109	4.2969280402054965E-4
110-111	3.8672352361849465E-4
112-113	8.808702482421267E-4
114-115	2.3633104221130227E-4
116-117	5.80085285427742E-4
118-119	2.1484640201027482E-4
120-121	8.593856080410993E-5
122-123	0.0016543172954791162
124-125	1.9336176180924733E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2327244.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.8572838890992
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.01147318659017	53.00887209328435
2	8.935776479029103	11.412288307817937
3	3.0408892325464696	5.825487809940748
4	1.4612708089198547	3.732511395361952
5	0.8949482947473473	2.8574483661873296
6	0.5307145920845627	2.0333995422498843
7	0.38627120844252866	1.7266361150989975
8	0.29641969419914116	1.5142845250235626
9	0.22234332555543707	1.2778416774755956
>10	1.1575602163855476	12.997119224020235
>50	0.04923198694406621	2.094238123820463
>100	0.012696799119463333	1.2112766864570401
>500	6.736257271272974E-5	0.02821843649566267
>1k	3.3681286356364385E-4	0.28037769676635155
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.296928040205496E-5
2	0.0	0.0	0.0	0.0	8.593856080410993E-5
3	0.0	0.0	0.0	0.0	8.593856080410993E-5
4	0.0	0.0	0.0	0.0	8.593856080410993E-5
5	0.0	0.0	0.0	0.0	8.593856080410993E-5
6	4.296928040205496E-5	0.0	0.0	0.0	8.593856080410993E-5
7	4.296928040205496E-5	0.0	0.0	0.0	8.593856080410993E-5
8	4.296928040205496E-5	0.0	0.0	0.0	8.593856080410993E-5
9	4.296928040205496E-5	0.0	0.0	0.0	8.593856080410993E-5
10-11	4.296928040205496E-5	0.0	0.0	0.0	8.593856080410993E-5
12-13	4.296928040205496E-5	0.0	0.0	0.0	8.593856080410993E-5
14-15	4.296928040205496E-5	0.0	0.0	0.0	1.7187712160821985E-4
16-17	4.296928040205496E-5	0.0	0.0	0.0	3.2226960301541226E-4
18-19	4.296928040205496E-5	0.0	0.0	4.296928040205496E-5	3.437542432164397E-4
20-21	4.296928040205496E-5	0.0	0.0	8.593856080410993E-5	3.437542432164397E-4
22-23	6.445392060308244E-5	0.0	0.0	1.0742320100513741E-4	3.8672352361849465E-4
24-25	8.593856080410993E-5	0.0	0.0	1.7187712160821985E-4	4.5117744422157715E-4
26-27	8.593856080410993E-5	0.0	0.0	6.230545658297969E-4	4.726620844226046E-4
28-29	8.593856080410993E-5	0.0	0.0	0.001611348015077061	5.156313648246595E-4
30-31	8.593856080410993E-5	0.0	0.0	0.008636825360813047	5.156313648246595E-4
32-33	8.593856080410993E-5	0.0	0.0	0.02591047608243914	5.586006452267145E-4
34-35	8.593856080410993E-5	0.0	0.0	0.044816959459343324	6.875084864328794E-4
36-37	8.593856080410993E-5	0.0	0.0	0.0730262920432924	6.875084864328794E-4
38-39	8.593856080410993E-5	0.0	0.0	0.12512654453078406	6.875084864328794E-4
40-41	8.593856080410993E-5	0.0	0.0	0.18708824687054731	6.875084864328794E-4
42-43	8.593856080410993E-5	0.0	0.0	0.246278430624378	6.875084864328794E-4
44-45	8.593856080410993E-5	0.0	0.0	0.308820218249569	6.875084864328794E-4
46-47	8.593856080410993E-5	0.0	0.0	0.3739401626988833	7.089931266339069E-4
48-49	8.593856080410993E-5	0.0	0.0	0.44630043089594384	8.164163276390443E-4
50-51	8.593856080410993E-5	2.148464020102748E-5	0.0	0.5246978829894932	8.164163276390443E-4
52-53	8.593856080410993E-5	4.296928040205496E-5	0.0	0.6064254543142017	9.238395286441817E-4
54-55	8.593856080410993E-5	8.593856080410993E-5	0.0	0.6956081957886668	9.88293449247264E-4
56-57	8.593856080410993E-5	8.593856080410993E-5	0.0	0.788056602573688	9.88293449247264E-4
58-59	8.593856080410993E-5	8.593856080410993E-5	0.0	0.8914836605014342	0.0010097780894482915
60-61	8.593856080410993E-5	1.0742320100513741E-4	0.0	0.9999381242362211	0.001031262729649319
62-63	8.593856080410993E-5	1.2890784120616488E-4	0.0	1.1241193445981599	0.001031262729649319
64-65	8.593856080410993E-5	1.2890784120616488E-4	0.0	1.2410387565721515	0.001031262729649319
66-67	8.593856080410993E-5	1.2890784120616488E-4	0.0	1.3640598063632348	0.0010527473698503467
68-69	8.593856080410993E-5	1.2890784120616488E-4	0.0	1.5074697797050933	0.001074232010051374
70-71	8.593856080410993E-5	1.2890784120616488E-4	0.0	1.667637772403753	0.001117201290453429
72-73	8.593856080410993E-5	1.2890784120616488E-4	0.0	1.8204150488732596	0.0012461091316595938
74-75	8.593856080410993E-5	1.2890784120616488E-4	0.0	1.9808408572543317	0.0012461091316595938
76-77	8.593856080410993E-5	1.2890784120616488E-4	0.0	2.1580891389128087	0.0012675937718606214
78-79	8.593856080410993E-5	1.2890784120616488E-4	0.0	2.35613455228588	0.0012890784120616488
80-81	8.593856080410993E-5	1.2890784120616488E-4	0.0	2.5572092999272957	0.0013105630522626762
82-83	8.593856080410993E-5	1.2890784120616488E-4	0.0	2.774010804195864	0.0013320476924637038
84-85	8.593856080410993E-5	1.2890784120616488E-4	0.0	2.9952596289860454	0.0013320476924637038
86-87	8.593856080410993E-5	1.2890784120616488E-4	0.0	3.231182463033528	0.0013320476924637038
88-89	8.593856080410993E-5	1.7187712160821985E-4	0.0	3.4980431789704904	0.0013535323326647314
90-91	8.593856080410993E-5	1.7187712160821985E-4	0.0	3.7779665561496776	0.0013750169728657588
92-93	8.593856080410993E-5	1.7187712160821985E-4	0.0	4.062509130972085	0.0014179862532678138
94-95	8.593856080410993E-5	1.7187712160821985E-4	0.0	4.362520646739233	0.0014609555336698688
96-97	8.593856080410993E-5	1.7187712160821985E-4	0.0	4.684768765114445	0.0014824401738708962
98-99	8.593856080410993E-5	1.7187712160821985E-4	0.0	5.035355123914811	0.001525409454272951
100-101	8.593856080410993E-5	1.7187712160821985E-4	0.0	5.409682010137312	0.0015468940944739786
102-103	8.593856080410993E-5	2.1484640201027482E-4	0.0	5.803452495741745	0.0015683787346750062
104-105	8.593856080410993E-5	2.1484640201027482E-4	0.0	6.217418543135142	0.0015898633748760336
106-107	8.593856080410993E-5	2.1484640201027482E-4	0.0	6.656951312367762	0.0015898633748760336
108-109	8.593856080410993E-5	2.1484640201027482E-4	0.0	7.11904295381146	0.0016328326552780886
110-111	8.593856080410993E-5	2.1484640201027482E-4	0.0	7.604423945233075	0.001740255856283226
112-113	8.593856080410993E-5	2.1484640201027482E-4	0.0	8.110602068369282	0.0018047097768863084
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2135	0.0	62.705627	1
GTATCAA	3930	0.0	54.13945	1
GTACATG	10980	0.0	42.83796	1
TACATGG	11595	0.0	40.602154	2
ACATGGG	11895	0.0	39.12725	3
TCAACGC	5250	0.0	38.139294	4
CATGGGG	10640	0.0	37.46944	4
CAACGCA	5580	0.0	36.310936	5
TATCAAC	5915	0.0	35.46342	2
ATCAACG	5680	0.0	35.461823	3
AACGCAG	5855	0.0	34.292007	6
ATGGGGG	6335	0.0	33.394463	5
TGGGGGG	4630	0.0	28.824179	6
ACGCAGA	7055	0.0	28.199795	7
CGCAGAG	7650	0.0	26.080412	8
ATGGGGA	3875	0.0	24.913572	5
AGAGTAC	7775	0.0	23.361343	10-11
GCAGAGT	8750	0.0	21.847885	9
CAGAGTA	8445	0.0	20.30978	9
ATTACGC	155	0.0056389556	19.223436	3
>>END_MODULE