FastQCFastQC Report
Mon 15 Aug 2016
SRR2921326_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921326_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2221796
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22750.062.256611
GTATCAA42200.056.266561
GTACATG145450.044.7603571
TCAACGC54050.043.045914
TACATGG150900.042.666682
CAACGCA55600.041.8458865
ACATGGG152600.041.7234343
AACGCAG58050.040.3863876
CATGGGG132150.039.804844
ATCAACG59450.039.1359333
ATGGGGG81100.039.107245
TATCAAC60500.038.555072
ACGCAGA69200.033.6142737
TGGGGGG61150.031.6248116
CGCAGAG74200.031.428638
GCAGAGT85700.027.0023969
AGAGTAC76100.025.5230210-11
GAGTACA94600.025.2256971
AGTACAT94200.024.7620142
GTAAGTA16950.024.2231272
Produced by FastQC (version 0.11.5)