FastQCFastQC Report
Mon 15 Aug 2016
SRR2921326_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921326_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2221796
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26990.12147829953785136No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24690.11112631402703038No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27450.073.596161
GTATCAA52550.053.457171
GTACATG129200.045.474911
TACATGG133100.044.376262
ACATGGG138650.042.2990233
CATGGGG123500.039.6697964
ATGGGGG75150.038.3065645
TCAACGC71750.037.297484
TATCAAC77950.035.7072032
CAACGCA74600.035.2334255
ATCAACG76700.034.8904083
AACGCAG77850.033.51856
TGGGGGG56400.032.111726
ACGCAGA94850.027.1901767
CGCAGAG99500.025.6754118
GTAAGTA12100.024.6286012
ATGGGGA40750.023.8404855
AGAGTAC100600.023.736410-11
GCAGAGT108100.022.751379
CAGAGTA106800.022.3034449
Produced by FastQC (version 0.11.5)