FastQCFastQC Report
Mon 15 Aug 2016
SRR2921325_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921325_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2102108
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17900.055.1842081
GTATCAA32850.052.531821
TCAACGC40100.042.4385724
CAACGCA40650.041.8643765
GTACATG106200.041.4635961
TACATGG109200.040.1046072
AACGCAG42800.039.760436
ACATGGG110250.039.0210383
ATCAACG43950.038.856363
TATCAAC45500.037.7942352
CATGGGG96400.037.3437084
ATGGGGG58650.033.2770585
ACGCAGA52400.032.130837
TAGTACG2307.2759576E-1231.0450632
CGCAGAG55550.030.0939168
AGTACAT69250.024.2308372
GCAGAGT66700.024.0803839
TGGGGGG41100.024.0322866
GAGTACA71400.023.5856571
ATGGGGA34500.023.4562685
Produced by FastQC (version 0.11.5)