##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921325_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2102108
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.509138921501656	31.0	31.0	34.0	27.0	34.0
2	30.598978739436795	33.0	31.0	34.0	27.0	34.0
3	30.639817744854213	33.0	31.0	34.0	27.0	34.0
4	34.07363798624999	37.0	35.0	37.0	30.0	37.0
5	34.14121063237474	37.0	35.0	37.0	30.0	37.0
6	34.093934755017344	37.0	35.0	37.0	30.0	37.0
7	34.109334534667106	37.0	35.0	37.0	30.0	37.0
8	34.09290959360794	37.0	35.0	37.0	30.0	37.0
9	35.69314136095767	39.0	37.0	39.0	30.0	39.0
10-11	35.71734040306207	39.0	37.0	39.0	30.0	39.0
12-13	35.65424611865803	39.0	37.0	39.0	30.0	39.0
14-15	36.973282295676526	41.0	38.0	41.0	30.0	41.0
16-17	36.954123194431496	41.0	38.0	41.0	30.0	41.0
18-19	36.93482708785657	41.0	38.0	41.0	30.0	41.0
20-21	36.96561213791109	41.0	38.0	41.0	30.0	41.0
22-23	36.90330254201972	41.0	38.0	41.0	30.0	41.0
24-25	36.87648327298122	41.0	38.0	41.0	30.0	41.0
26-27	36.84209755160059	41.0	38.0	41.0	30.0	41.0
28-29	36.772007670395624	40.0	38.0	41.0	30.0	41.0
30-31	36.6914915884436	40.0	38.0	41.0	30.0	41.0
32-33	36.615404394065386	40.0	38.0	41.0	30.0	41.0
34-35	36.505523740930535	40.0	38.0	41.0	29.5	41.0
36-37	36.394596043590525	40.0	37.0	41.0	29.0	41.0
38-39	36.29593650754386	40.0	37.0	41.0	28.0	41.0
40-41	36.19812136198521	40.0	37.0	41.0	28.0	41.0
42-43	36.00230316425227	40.0	36.5	41.0	27.0	41.0
44-45	35.74424577614471	40.0	36.0	41.0	26.5	41.0
46-47	35.51983175935775	40.0	35.5	41.0	26.0	41.0
48-49	35.31589980153255	40.0	35.0	41.0	25.5	41.0
50-51	35.137133534528196	39.0	35.0	41.0	24.5	41.0
52-53	34.93877288892864	39.0	35.0	41.0	24.0	41.0
54-55	34.7258000064697	39.0	35.0	41.0	24.0	41.0
56-57	34.47955790092612	38.5	34.5	40.0	23.0	41.0
58-59	34.209993254390355	38.0	34.0	40.0	22.5	41.0
60-61	33.9228971584714	37.5	34.0	40.0	22.0	41.0
62-63	33.60385099148093	37.0	34.0	40.0	21.5	41.0
64-65	33.2569006920672	36.5	33.5	39.5	20.5	41.0
66-67	32.917277323524765	36.0	33.0	39.0	20.0	41.0
68-69	32.56899217357053	35.5	33.0	39.0	19.0	40.0
70-71	32.51755594860017	35.0	33.0	38.5	20.0	40.0
72-73	32.22489971970993	35.0	33.0	37.0	18.5	39.5
74-75	31.902202693677012	35.0	33.0	37.0	18.0	39.0
76-77	31.603028483788655	35.0	33.0	36.5	17.0	39.0
78-79	31.313299554542393	35.0	33.0	36.0	14.0	37.5
80-81	31.03739341651333	35.0	32.5	35.5	11.0	37.0
82-83	30.81159245861773	35.0	32.0	35.0	8.5	37.0
84-85	30.59358962527139	35.0	32.0	35.0	7.0	36.0
86-87	30.424877789342887	35.0	32.0	35.0	6.5	36.0
88-89	30.282905064820646	35.0	32.0	35.0	2.0	36.0
90-91	30.156679390402395	35.0	32.0	35.0	2.0	35.0
92-93	30.040094514649105	35.0	32.0	35.0	2.0	35.0
94-95	29.91268621783467	35.0	31.5	35.0	2.0	35.0
96-97	29.814041428889475	35.0	31.0	35.0	2.0	35.0
98-99	29.722943825911894	35.0	31.0	35.0	2.0	35.0
100-101	29.613791251448546	35.0	31.0	35.0	2.0	35.0
102-103	28.751011365733824	33.5	29.5	34.5	2.0	35.0
104-105	29.34621793932567	34.0	31.0	35.0	2.0	35.0
106-107	29.420482915245078	34.5	31.0	35.0	2.0	35.0
108-109	29.343127232283024	34.5	31.0	35.0	2.0	35.0
110-111	29.248832124705295	34.0	31.0	35.0	2.0	35.0
112-113	29.153731872958	34.0	31.0	35.0	2.0	35.0
114-115	29.0187497502507	34.0	30.5	35.0	2.0	35.0
116-117	28.87831762212027	34.0	30.0	35.0	2.0	35.0
118-119	28.866336791449346	34.5	30.5	35.0	2.0	35.0
120-121	29.026141853796283	35.0	31.0	35.0	2.0	35.0
122-123	28.884421494994548	35.0	31.0	35.0	2.0	35.0
124-125	28.31264069210526	34.5	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	80122.0
3	11222.0
4	6932.0
5	5637.0
6	5973.0
7	6599.0
8	6913.0
9	7384.0
10	8122.0
11	8342.0
12	8382.0
13	8285.0
14	8453.0
15	7780.0
16	6545.0
17	5913.0
18	5709.0
19	5549.0
20	5617.0
21	5979.0
22	6951.0
23	8719.0
24	11783.0
25	14998.0
26	16913.0
27	19084.0
28	23044.0
29	27974.0
30	33874.0
31	42552.0
32	55179.0
33	74513.0
34	112855.0
35	186679.0
36	395328.0
37	670082.0
38	186034.0
39	88.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.24939430907461	17.318811342264382	11.720904473072686	25.71088987558832
2	16.211386507924423	19.687362026704015	39.11366906200996	24.9875824033616
3	19.189159743066767	25.0445674778019	28.30699724863919	27.45927553049215
4	13.641378791908501	15.161353708777812	35.37230512163082	35.824962377682866
5	14.427502815279153	37.68358783837467	33.27971576503992	14.609193581306263
6	33.25909799302854	34.89127615784527	17.54214402131887	14.307481827807322
7	29.739071768823354	31.602661332835986	20.60634578676223	18.051921111578434
8	27.8275813178521	34.65477915743258	19.45371950134503	18.06392002337029
9	28.435749060258136	15.485556687332029	18.32202851981558	37.75666573259426
10-11	26.58516702375886	25.21314324467757	26.92502110558788	21.27666862597568
12-13	26.498867244478237	22.675329717015693	27.06752859482177	23.758274443684304
14-15	24.113677115431184	23.810223832774096	24.120932210767894	27.95516684102683
16-17	24.54751620949292	26.705761012440952	24.688595529324566	24.058127248741563
18-19	24.61373122058413	26.27146261433922	25.73437096360603	23.380435201470622
20-21	25.456377920690244	25.397909405676344	25.399431775473293	23.746280898160123
22-23	25.37722676422525	24.95579178728678	25.32760700004995	24.339374448438026
24-25	24.810273941972753	25.16791327026258	25.381188575712972	24.640624212051694
26-27	24.55474112247459	25.434067195288314	25.436208005918154	24.574983676318947
28-29	24.593013326051285	25.71798020240983	25.34323704443397	24.345769427104916
30-31	25.113862529564297	25.428374539988717	25.279824584981874	24.177938345465112
32-33	24.78202693010803	25.492685862585322	25.266107575326874	24.459179631979772
34-35	24.76810630976344	25.640684075087073	25.431448100391403	24.15976151475808
36-37	24.846219700957707	25.508511527545817	25.397828248000003	24.24744052349647
38-39	24.83685395140039	25.732117447076547	25.212814190245158	24.218214411277902
40-41	24.825007481159133	25.505210169428626	25.276208201253407	24.393574148158827
42-43	24.577236395087784	25.512918285605267	25.221188205389012	24.688657113917934
44-45	24.39954946436451	25.573511168835495	25.483546766859703	24.543392599940294
46-47	24.44641779037167	25.30269027894273	25.525322077490802	24.7255698531948
48-49	24.40719534329409	25.522773280556066	25.49734408860492	24.572687287544927
50-51	24.425060080243373	25.62414351607924	25.467553100516394	24.483243303160997
52-53	24.583680622951942	25.39645726529882	25.413155711344288	24.60670640040495
54-55	24.257820351646732	25.347204464388724	25.693689364845078	24.701285819119466
56-57	24.385917462302025	25.511284475054314	25.32947593802192	24.773322124621746
58-59	24.483455347135134	25.43723726752517	25.5011303800094	24.578177005330293
60-61	24.786065976004185	25.52515079152318	25.467300890607785	24.221482341864846
62-63	24.547349942256893	25.597287322801215	25.507943096576685	24.34741963836521
64-65	24.494363901950994	25.279509502835474	25.510595588408446	24.715531006805087
66-67	24.493582845469746	25.463886676733168	25.417740632590906	24.624789845206188
68-69	24.40658404066868	25.452131259302323	25.51393067855905	24.627354021469948
70-71	24.348014726905717	25.24035408132148	25.682765438488907	24.72886575328389
72-73	24.39943891892226	25.709754004910053	25.29593649136887	24.594870584798816
74-75	24.310603937829356	25.66406329908217	25.4169677657835	24.608364997304975
76-77	24.29363425807543	25.219367467178433	25.534351771483433	24.952646503262713
78-79	24.221922513367964	25.3358166352686	25.695919202298718	24.746341649064718
80-81	24.495852718276705	25.524877149073554	25.308634780649935	24.67063535199981
82-83	24.517612111564702	25.492449777377736	25.5033442103225	24.486593900735066
84-85	24.458733120906228	25.63885727063284	25.259961534756098	24.64244807370483
86-87	24.18897314136234	25.708086707125933	25.47344738777026	24.62949276374147
88-89	24.142799136917837	25.415062739013266	25.548031245458198	24.894106878610696
90-91	24.431694111255368	25.30412803467947	25.590070946953674	24.67410690711149
92-93	24.512994272871644	25.39244959146992	25.531417376253966	24.56313875940447
94-95	24.47335832281533	25.31069944066251	25.501997905755175	24.71394433076699
96-97	24.28772539801027	25.23215977216728	25.635462889880696	24.844651939941755
98-99	24.135138066770253	25.315063983322183	25.462379290888382	25.08741865901918
100-101	24.218898859400248	25.352195765729483	25.6462363823941	24.782668992476168
102-103	24.069362924375145	25.294203700069577	25.73374009122319	24.902693284332088
104-105	24.12509431502789	25.42916311052627	25.504710280409398	24.941032294036436
106-107	24.157367917883498	25.24895447801686	25.682839262100902	24.91083834199874
108-109	24.103702604197146	25.242714751471606	25.689632451344057	24.963950192987188
110-111	23.971624187473328	25.657288464749794	25.6507468608627	24.720340486914175
112-113	23.935968403327603	25.63487946085466	25.67457986906239	24.754572266755346
114-115	24.046624783902253	25.784041163469322	25.520204485318676	24.64912956730975
116-117	23.822366187949964	25.71742687532394	25.66545364070725	24.79475329601885
118-119	23.93619935913471	25.421148554479505	25.73757876806156	24.90507331832422
120-121	23.78746623822055	25.556995022025742	25.87216839949778	24.783370340255935
122-123	23.766206983658208	25.70515795305044	25.759580640841424	24.76905442244993
124-125	23.778848889239864	25.674143726624276	25.67873443085258	24.86827295328328
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	454.0
1	382.5
2	274.5
3	216.0
4	190.5
5	177.5
6	171.5
7	211.0
8	287.0
9	307.5
10	304.0
11	310.0
12	319.5
13	342.5
14	376.5
15	419.0
16	463.0
17	525.5
18	557.5
19	592.0
20	666.0
21	741.5
22	857.5
23	1043.5
24	1244.5
25	1545.0
26	2067.5
27	2864.0
28	3916.5
29	5471.0
30	7760.5
31	10384.5
32	13802.0
33	17380.5
34	21289.0
35	25586.0
36	30571.0
37	35792.5
38	39992.0
39	44561.0
40	49281.5
41	53284.0
42	57033.0
43	62337.0
44	68560.5
45	73886.5
46	78736.0
47	83716.5
48	88811.0
49	93292.5
50	95543.0
51	96646.5
52	96405.5
53	95018.5
54	93781.0
55	90134.5
56	84319.0
57	77427.5
58	70359.5
59	62836.0
60	54219.0
61	45986.5
62	37790.0
63	29243.0
64	21676.5
65	16219.0
66	12312.0
67	9389.5
68	7252.0
69	5592.5
70	4374.0
71	3330.0
72	2459.0
73	1900.5
74	1427.0
75	1029.5
76	735.0
77	452.0
78	297.5
79	216.5
80	132.0
81	100.0
82	82.5
83	58.5
84	45.5
85	36.0
86	27.5
87	20.0
88	19.5
89	15.5
90	9.5
91	9.5
92	10.5
93	8.5
94	5.0
95	4.0
96	3.5
97	5.5
98	4.5
99	1.5
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017886806957587334
2	0.011987966365191513
3	0.01136953952889195
4	0.014128674644689997
5	0.008800689593493768
6	0.032491194553276995
7	0.01446167371038976
8	0.0139859607593901
9	0.00956183031509323
10-11	0.008396333585144055
12-13	0.007325979445394813
14-15	0.006517267428695386
16-17	0.0047095582148966655
18-19	0.0066124100188953185
20-21	0.0058036980021958905
22-23	0.005851269297295857
24-25	0.006184268362995621
26-27	0.004780915157546615
28-29	0.006422124838495452
30-31	0.005779912354645908
32-33	0.008824475241043753
34-35	0.008324976642494106
36-37	0.00782547804394446
38-39	0.007468693330694711
40-41	0.0076589785110945776
42-43	0.007849263691494444
44-45	0.007873049339044426
46-47	0.009847258085693028
48-49	0.0091812599542935
50-51	0.006065340125245706
52-53	0.005518270231596093
54-55	0.006683766961545269
56-57	0.008158477109644225
58-59	0.007611407215994611
60-61	0.005542055879146076
62-63	0.005970197535045773
64-65	0.00368677537024739
66-67	0.004091131378597104
68-69	0.0069691947321450665
70-71	0.0049949859854964635
72-73	0.004495487386946817
74-75	0.00413870267369707
76-77	0.004685772567346682
78-79	0.0032348480667977095
80-81	0.003258633714347693
82-83	0.005565841526696059
84-85	0.01001375761854291
86-87	0.0075400502733446615
88-89	0.005066342928146413
90-91	0.005470698936496127
92-93	0.00865797570819387
94-95	0.007468693330694711
96-97	0.010227828446492759
98-99	0.007325979445394813
100-101	0.008919617831243686
102-103	0.005732341059545941
104-105	0.0052328424609962955
106-107	0.007968191929244358
108-109	0.00737355074049478
110-111	0.008420119232694038
112-113	0.005446913288946143
114-115	0.0023785647549983157
116-117	0.003068348533947827
118-119	0.16093369132318605
120-121	0.011845252479891613
122-123	0.002069351336848535
124-125	0.0016174240333988548
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2102108.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.130507907778345
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.42424363430949	49.163748614642486
2	10.453902539742728	12.78104743745774
3	3.4495491055450875	6.326180666243812
4	1.6878015202397216	4.127046567190985
5	0.9790438362647553	2.9924723487422136
6	0.632747429564629	2.320810304797618
7	0.4464212984420018	1.9102972500226627
8	0.3265536488057629	1.5969912328507652
9	0.24454149549838575	1.3454051241909613
>10	1.2860339088297656	13.734085874746182
>50	0.05540017085614964	2.28031843558633
>100	0.013450743424883919	1.2797784482022032
>500	2.330013575193476E-4	0.09414947768045397
>1k	7.766711917310482E-5	0.0476682176455699
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.514259019993263E-5
2	0.0	0.0	0.0	0.0	9.514259019993263E-5
3	0.0	0.0	0.0	0.0	9.514259019993263E-5
4	0.0	0.0	0.0	0.0	9.514259019993263E-5
5	0.0	0.0	0.0	0.0	9.514259019993263E-5
6	0.0	0.0	0.0	0.0	1.9028518039986526E-4
7	0.0	0.0	0.0	0.0	1.9028518039986526E-4
8	0.0	0.0	0.0	0.0	2.378564754998316E-4
9	0.0	0.0	0.0	0.0	2.8542777059979794E-4
10-11	0.0	0.0	0.0	0.0	2.8542777059979794E-4
12-13	0.0	0.0	0.0	0.0	5.470698936496127E-4
14-15	0.0	0.0	0.0	0.0	7.37355074049478E-4
16-17	4.7571295099966316E-5	0.0	0.0	0.0	7.611407215994611E-4
18-19	4.7571295099966316E-5	0.0	0.0	0.0	8.087120166994274E-4
20-21	4.7571295099966316E-5	0.0	0.0	1.1892823774991581E-4	8.562833117993937E-4
22-23	4.7571295099966316E-5	0.0	0.0	1.9028518039986526E-4	9.0385460689936E-4
24-25	4.7571295099966316E-5	0.0	0.0	4.757129509996632E-4	9.514259019993264E-4
26-27	4.7571295099966316E-5	0.0	0.0	7.849263691494443E-4	0.0010703541397492423
28-29	4.7571295099966316E-5	0.0	0.0	0.003567847132497474	0.0011417110823991918
30-31	4.7571295099966316E-5	0.0	0.0	0.012772892734340957	0.001189282377499158
32-33	4.7571295099966316E-5	0.0	0.0	0.03115919829047794	0.0012368536725991244
34-35	4.7571295099966316E-5	0.0	0.0	0.05332742180706224	0.0012844249676990907
36-37	4.7571295099966316E-5	0.0	0.0	0.08734089780353815	0.0012844249676990907
38-39	4.7571295099966316E-5	0.0	0.0	0.15049179204874347	0.0013082106152490739
40-41	4.7571295099966316E-5	0.0	0.0	0.22767621834843882	0.001331996262799057
42-43	4.7571295099966316E-5	0.0	0.0	0.29686866707133985	0.001331996262799057
44-45	4.7571295099966316E-5	0.0	0.0	0.36872510831983896	0.001331996262799057
46-47	4.7571295099966316E-5	0.0	0.0	0.44267468655273656	0.001331996262799057
48-49	4.7571295099966316E-5	0.0	0.0	0.5297301565856749	0.001331996262799057
50-51	4.7571295099966316E-5	0.0	0.0	0.6228271810963091	0.001331996262799057
52-53	4.7571295099966316E-5	0.0	0.0	0.7198964087477904	0.0013795675578990234
54-55	4.7571295099966316E-5	0.0	0.0	0.8223887640406677	0.0014271388529989897
56-57	4.7571295099966316E-5	0.0	0.0	0.9275926831542433	0.0014509245005489729
58-59	4.7571295099966316E-5	0.0	0.0	1.0472107046831085	0.001474710148098956
60-61	4.7571295099966316E-5	0.0	0.0	1.17208535432052	0.001474710148098956
62-63	4.7571295099966316E-5	0.0	0.0	1.3075446171176743	0.001474710148098956
64-65	4.7571295099966316E-5	0.0	0.0	1.436415255543483	0.0015698527382988884
66-67	4.7571295099966316E-5	0.0	0.0	1.5799140672125316	0.0015698527382988884
68-69	4.7571295099966316E-5	0.0	0.0	1.738516765075819	0.0015698527382988884
70-71	4.7571295099966316E-5	0.0	0.0	1.913721844928995	0.001641209680948838
72-73	4.7571295099966316E-5	0.0	0.0	2.0857158623629233	0.0018552805088986864
74-75	4.7571295099966316E-5	0.0	0.0	2.2576385228542017	0.0019028518039986527
76-77	4.7571295099966316E-5	0.0	0.0	2.4496838411727655	0.0019028518039986527
78-79	4.7571295099966316E-5	0.0	0.0	2.66955836712481	0.0019028518039986527
80-81	4.7571295099966316E-5	0.0	0.0	2.8910503171102535	0.0019504230990986192
82-83	4.7571295099966316E-5	0.0	0.0	3.123911806624588	0.002093136984398518
84-85	4.7571295099966316E-5	0.0	0.0	3.3616017825915696	0.0021407082794984845
86-87	4.7571295099966316E-5	0.0	0.0	3.6210794117143363	0.0021644939270484675
88-89	4.7571295099966316E-5	0.0	0.0	3.9103842428647813	0.002188279574598451
90-91	7.135694264994947E-5	0.0	0.0	4.213770177364816	0.002188279574598451
92-93	9.514259019993263E-5	0.0	0.0	4.517346397045252	0.002188279574598451
94-95	9.514259019993263E-5	0.0	0.0	4.831079088229529	0.00233099345989835
96-97	9.514259019993263E-5	0.0	0.0	5.167455715881392	0.0024023504025482995
98-99	9.514259019993263E-5	0.0	0.0	5.539225387087628	0.002497492992748232
100-101	9.514259019993263E-5	0.0	0.0	5.945650746774191	0.002521278640298215
102-103	9.514259019993263E-5	0.0	0.0	6.365705282506893	0.0026877781731480975
104-105	9.514259019993263E-5	0.0	0.0	6.802647628000084	0.0027115638206980804
106-107	1.4271388529989897E-4	0.0	0.0	7.2673002528890045	0.0027591351157980467
108-109	1.6649953284988213E-4	0.0	0.0	7.747318406095215	0.002830492058447996
110-111	1.9028518039986526E-4	0.0	0.0	8.256997261796254	0.0028780633535479628
112-113	1.9028518039986526E-4	0.0	0.0	8.78682256097213	0.0029018490010979457
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1790	0.0	55.184208	1
GTATCAA	3285	0.0	52.53182	1
TCAACGC	4010	0.0	42.438572	4
CAACGCA	4065	0.0	41.864376	5
GTACATG	10620	0.0	41.463596	1
TACATGG	10920	0.0	40.104607	2
AACGCAG	4280	0.0	39.76043	6
ACATGGG	11025	0.0	39.021038	3
ATCAACG	4395	0.0	38.85636	3
TATCAAC	4550	0.0	37.794235	2
CATGGGG	9640	0.0	37.343708	4
ATGGGGG	5865	0.0	33.277058	5
ACGCAGA	5240	0.0	32.13083	7
TAGTACG	230	7.2759576E-12	31.045063	2
CGCAGAG	5555	0.0	30.093916	8
AGTACAT	6925	0.0	24.230837	2
GCAGAGT	6670	0.0	24.080383	9
TGGGGGG	4110	0.0	24.032286	6
GAGTACA	7140	0.0	23.585657	1
ATGGGGA	3450	0.0	23.456268	5
>>END_MODULE