FastQCFastQC Report
Mon 15 Aug 2016
SRR2921325_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921325_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2102108
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20600.056.8529471
GTATCAA37150.050.826761
GTACATG101050.042.1024931
ACATGGG104400.040.927193
TACATGG105600.040.0670742
CATGGGG94850.038.2624864
ATGGGGG58050.035.6221775
TCAACGC50000.035.2788934
CAACGCA52050.033.0879865
TATCAAC55750.032.709192
ATCAACG54900.032.2386863
AACGCAG55150.031.011995
ATGGGGA35850.025.931565
TGGGGGG41900.025.6037436
ACGCAGA67400.025.2804937
CGCAGAG71700.023.5981857
AGAGTAC74550.022.3324310-11
CAGAGTA80350.022.0926469
GTAAGTA9150.021.4924562
GCAGAGT83550.020.96188
Produced by FastQC (version 0.11.5)